Foxe1 (NM_138909) Rat Tagged ORF Clone

CAT#: RR207960

Foxe1 (Myc-DDK-tagged ORF) - Rat forkhead box E1 (thyroid transcription factor 2) (Foxe1), (10 ug)

Product Images

Circular map for RR207960

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Foxe1
• Synonyms: Ttf-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207960 representing NM_138909
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGCCGAGAGCGCGCCGCCGCCCCCGCCGCAGCCCGAGGCGCTGGCGGCCGTGAAGGAAGAGCGCG
  GTGAGGCGGCGGCGGGCGCGGGGGTCCCGGCGGAGGTGGCGGGTCGCGGCGCCGGAGGACGGCGACGCAA
  GCGCCCCCTGCAGCGGGGGAAGCCGCCGTACAGCTACATCGCACTCATCGCGATGGCTATCGCGCACGCG
  CCCGAGCGCCGCCTGACTCTGGGCGGCATCTACAAGTTCATCACCGAGCGCTTCCCGTTCTACCGCGACA
  ACCCCAAGAAGTGGCAGAACAGCATCCGCCACAACCTCACCCTCAACGACTGCTTCCTCAAGATCCCGCG
  CGAGGCGGGCCGCCCGGGCAAAGGCAACTACTGGGCGCTCGACCCCAACGCCGAGGACATGTTCGAGAGC
  GGCAGCTTCCTGCGCCGGCGCAAGCGCTTCAAGCGCTCGGACCTGTCCACCTACCCGGCGTACATGCATG
  ATGCAGCCGCTGCTGCCGCCGCCGCCGCCGCCGCCATCTTCCCGGGTGCCGTGCCTGCCGCGAGGCCGGC
  CTACCCGGGAGCCGTCTATGCGGGCTACGCGCCGCCGCTCGCCGCACCCCCGCCGGTCTACTACCCCGCA
  GCGTCGCCCGGGCCCTGCCGGGTCTTCGGCTTGGTTCCCGAGCGGCCGCTCAGCCCGGATCTGGGCCCCG
  CACCATCAGCAGCCGGAGGTTCGTGCGCCTTCGCGGCTGCGGCGGGCGCGCCGGGCACAGGGAGCTTCCA
  GCCCGCGGTATGCACGGGCGCGCGGCCGGTCAACCCGGCTGCCTATGCCGCTGCCTACGCGGGCCCCGAC
  GGCGCGTACCCGCAAGGGGCGAGCAGCGCGCTCTTCGCCGCTGCTGCGGGCCGTCTGGCCGGACCCACTT
  CGCCCACCGCGGGAGGAGGCAGCGGCGGCGTGGAGGCCACGGTGGACTTCTACGGGCGCACGTCTCCTGG
  CCAGTTCGGAGCGGCGCTGGGGCCCTGCTATAATCCCAGTGGGCAACTCGGAGCCGGTGGTGGAGGTGCC
  TATCACTCCCGCCACGCCACCGCCTACCCGGGCGCGGTGGATCGGTTCGTGTCTGCCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207960 representing NM_138909
  Red=Cloning site Green=Tags(s)
  
  MTAESAPPPPPQPEALAAVKEERGEAAAGAGVPAEVAGRGAGGRRRKRPLQRGKPPYSYIALIAMAIAHA
  PERRLTLGGIYKFITERFPFYRDNPKKWQNSIRHNLTLNDCFLKIPREAGRPGKGNYWALDPNAEDMFES
  GSFLRRRKRFKRSDLSTYPAYMHDAAAAAAAAAAAIFPGAVPAARPAYPGAVYAGYAPPLAAPPPVYYPA
  ASPGPCRVFGLVPERPLSPDLGPAPSAAGGSCAFAAAAGAPGTGSFQPAVCTGARPVNPAAYAAAYAGPD
  GAYPQGASSALFAAAAGRLAGPTSPTAGGGSGGVEATVDFYGRTSPGQFGAALGPCYNPSGQLGAGGGGA
  YHSRHATAYPGAVDRFVSAM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138909
• ORF Size: 1110 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138909.1, NP_620264.1
• RefSeq Size: 2807 bp
• RefSeq ORF: 1113 bp
• Locus ID: 192274
• Cytogenetics: 5q22
• MW: 37.8 kDa
• Gene Summary: transcription factor responsible for the development of a negative controller of thyroid-specific gene expression [RGD, Feb 2006]