Hnrnpa1 (NM_017248) Rat Tagged ORF Clone

CAT#: RR207899

Hnrnpa1 (Myc-DDK-tagged ORF) - Rat heterogeneous nuclear ribonucleoprotein A1 (Hnrnpa1), (10 ug)

Product Images

Circular map for RR207899

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hnrnpa1
• Synonyms: hnRNP A1; Hnrpa1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207899 representing NM_017248
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTAAGTCAGAGTCCCCCAAGGAACCGGAACAGCTGCGGAAGCTCTTCATTGGAGGGCTGAGCTTCG
  AAACAACCGACGAGAGTCTGAGGAGCCATTTTGAGCAATGGGGAACACTCACCGACTGTGTGGTAATGAG
  AGATCCAAACACCAAAAGATCCAGAGGCTTTGGGTTTGTCACATATGCCACTGTGGAGGAAGTGGATGCT
  GCCATGAATGCAAGACCACACAAAGTGGATGGAAGAGTTGTGGAACCTAAGAGAGCTGTGTCAAGAGAAG
  ATTCTCAGAGACCAGGTGCCCACTTAACTGTGAAGAAGATCTTTGTTGGCGGTATTAAAGAAGACACTGA
  AGAACATCACCTACGAGATTATTTTGAGCAGTATGGGAAAATTGAAGTGATTGAAATTATGACTGACAGA
  GGCAGTGGAAAAAAGAGGGGATTTGCGTTTGTCACCTTTGATGACCATGACTCTGTGGATAAGATTGTTA
  TTCAGAAATACCATACTGTGAATGGCCACAACTGTGAAGTAAGAAAGGCTCTGTGCAAACAAGAGATGGC
  TAGTGCTTCATCCAGCCAGAGAGGTCGAAGTGGTTCCGGAAACTTTGGTGGTGGTCGTGGAGGTGGTTTC
  GGTGGCAATGACAATTTTGGTCGAGGAGGGAACTTCAGTGGTCGTGGTGGCTTTGGTGGCAGCCGTGGTG
  GTGGTGGATATGGTGGCAGTGGGGATGGCTATAATGGATTTGGCAATGATGGAAGCAATTTTGGAGGTGG
  TGGAAGCTACAATGATTTTGGCAATTACAACAACCAGTCATCAAATTTTGGACCGATGAAAGGAGGAAAC
  TTTGGAGGCAGGAGCTCTGGCCCTTATGGTGGTGGAGGCCAGTACTTTGCTAAACCACGAAACCAAGGTG
  GCTATGGAGGTTCCAGCAGCAGCAGTAGCTATGGCAGTGGCAGGAGGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207899 representing NM_017248
  Red=Cloning site Green=Tags(s)
  
  MSKSESPKEPEQLRKLFIGGLSFETTDESLRSHFEQWGTLTDCVVMRDPNTKRSRGFGFVTYATVEEVDA
  AMNARPHKVDGRVVEPKRAVSREDSQRPGAHLTVKKIFVGGIKEDTEEHHLRDYFEQYGKIEVIEIMTDR
  GSGKKRGFAFVTFDDHDSVDKIVIQKYHTVNGHNCEVRKALCKQEMASASSSQRGRSGSGNFGGGRGGGF
  GGNDNFGRGGNFSGRGGFGGSRGGGGYGGSGDGYNGFGNDGSNFGGGGSYNDFGNYNNQSSNFGPMKGGN
  FGGRSSGPYGGGGQYFAKPRNQGGYGGSSSSSSYGSGRRF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017248
• ORF Size: 960 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017248.1, NP_058944.1
• RefSeq Size: 1696 bp
• RefSeq ORF: 963 bp
• Locus ID: 29578
• UniProt ID: P04256
• Cytogenetics: 7q36
• MW: 34.2 kDa
• Gene Summary: involved in the packaging of pre-mRNA into heterologous nuclear ribonucleoprotein particles and in the transport of poly-A mRNA from the nucleus to the cytoplasm; may modulate splice site selection [RGD, Feb 2006]