Add3 (NM_031552) Rat Tagged ORF Clone

CAT#: RR207822

Add3 (Myc-DDK-tagged ORF) - Rat adducin 3 (gamma) (Add3), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Add3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207822 representing NM_031552
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCAGACACCAGCCAAGCGGTGATCACCACCCCTCCTCCTCCCAGCATGCCTCACAAAGAGAGGT
  ATTTTGACCGAATCAATGAAAGTGACCCAGAATACCTTCGAGAGAGGAACATGTCTCCTGATCTACGGCA
  GGACTTCAATATGATGGAGCAGAGGAAGCGAGTCACTCAGATCCTGCAGAGTCCTGCCTTTCGGGAAGAC
  CTAGAGTGCCTCATCCAAGAACAGATGAAGAAAGGCCACGACCCGACCGGATTGCTAGCGTTACAGCAGA
  TCGCCGACTACATCATGGCCAACTCCTTCACCGGTTTTTCTTCACCTCCCCTGAGTCTTGGCATGGTCAC
  TCCTATTAATGACCTTCCTGGTGCAGACACATCCTCGTATGTGAAGGGAGAGAAGCTCACTCGTTGCAAA
  CTCGCCAGCCTGTACAGACTCGCAGATCTGTTTGGGTGGGCACATCTGGCAAATACATACATCTCGGTAC
  GAGTAAGTAAGGAGCAGGACCACATCATAATCATCCCCAGAGGCCTCTCTTTCTCTGAAGCCACAGCCTC
  TGCTCTGGTGAAGGTCAACATAATAGGAGAAGTGGTGGACCAGGGGAGTACTAATTTGAAAATTGACCAC
  TCAGGATTCAGTCCCCATGCTGCAATCTATTCAACCCGTCCCGATGTGAAGTGTGTGATACACATCCACA
  CCCTCGCTACAGCAGCTGTGTCTTCCATGAAGTGTGGGATCCTTCCAATTTCCCAAGAGTCTCTCATCCT
  GGGAGATGTAGCCTATTATGACTACCAGGGGTCGCTTGATGAGGAGGAGGAAAGAATCGAACTTCAGAAA
  GTTCTGGGTCCAAGCTGTAAGGTGCTGGTCCTCAGGAACCATGGCGTGGTAGCTCTTGGAGAAACACTTG
  AGGAGGCTTTCCACTACATCTTCAATGTACAGATGGCCTGTGAGATTCAGGTGCAGGCTGTAGCAGGGGC
  AGGGGGAGTGGACAATCTTCTCATACTGGATCTTCAGAAATACAAAGCTTTCACCCACGGTGTTGCCATG
  ACTGGAGGAGGCGGTGTGAATATGGGTTCCCATCAGAAGTGGAAAGTTGGCGAGATTGAGTTTGAAGGGC
  TCATGAGAACCCTGGACAACTTGGGCTACAGAACAGGCTACGCATACAGGCATCCTCTAGTTCGAGAGAA
  GCCTCGGCACAAGAGTGACGTGGAGATCCCAGCAACTGTGACTGCATTCTCCTTTGAAGATGATAGTGTG
  CCACTCTCTCCCCTCAAGTACATGGCACAGAGACAGCAGCGTGAAAAAACAAGATGGCTGAATTCTCCAA
  ATACATACATGAAAGTGAATGTGCCGGAGGAGTCCCGGAACGGGGAAACTAGTCCCAGGACCAAAATAAC
  GTGGATGAAAGCAGAGGACCCCTCTAAAGTTAGTAGTGGAACACCTATCAAAATTGAAGATCCCAATCAG
  TTTGTTCCTCTAAACACGAACCCGACTGAGGTGCTGGAGAAGAGAAATAAGATTCGGGAACAGAATCGCT
  ATGACCTGAAGACAGCAGGGCCACAGTCTCAGCTGCTGGCGGGGATCGTTGTGGACAAACCGCCTTCTAC
  CATGCGGTTTGAAGACGATGATCAGGGCCCACCAGCCCCTCCTAACCCATTCAGTCATCTCATGGAAGGA
  GAACTTGAAGAATATACGAAGACAATCGAGAGAAAGCAGCAAGGCCTGGACGAAAACCACGAGCTGTTTT
  CCAAGAGCTTCACCTCTGTGGATGTGCCTGTCATTGTGAATGGCAAGGACGAGATGCACGATGTTGAAGA
  CGAGCTCGCTCAGCGCGTCAGCAGGCTCACGACGAGCACCACCATAGAGAACATCGAGATCACCATCAAG
  TCCCCAGACAGAACTGAAGAGGTCCTGTCACCCGATGGCTCGCCTTCAAAGTCACCATCCAAGAAAAAGA
  AGAAATTCCGCACACCTTCTTTTCTCAAAAAGAACAAAAAAAAGGAGAAAGTTGAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207822 representing NM_031552
  Red=Cloning site Green=Tags(s)
  
  MSSDTSQAVITTPPPPSMPHKERYFDRINESDPEYLRERNMSPDLRQDFNMMEQRKRVTQILQSPAFRED
  LECLIQEQMKKGHDPTGLLALQQIADYIMANSFTGFSSPPLSLGMVTPINDLPGADTSSYVKGEKLTRCK
  LASLYRLADLFGWAHLANTYISVRVSKEQDHIIIIPRGLSFSEATASALVKVNIIGEVVDQGSTNLKIDH
  SGFSPHAAIYSTRPDVKCVIHIHTLATAAVSSMKCGILPISQESLILGDVAYYDYQGSLDEEEERIELQK
  VLGPSCKVLVLRNHGVVALGETLEEAFHYIFNVQMACEIQVQAVAGAGGVDNLLILDLQKYKAFTHGVAM
  TGGGGVNMGSHQKWKVGEIEFEGLMRTLDNLGYRTGYAYRHPLVREKPRHKSDVEIPATVTAFSFEDDSV
  PLSPLKYMAQRQQREKTRWLNSPNTYMKVNVPEESRNGETSPRTKITWMKAEDPSKVSSGTPIKIEDPNQ
  FVPLNTNPTEVLEKRNKIREQNRYDLKTAGPQSQLLAGIVVDKPPSTMRFEDDDQGPPAPPNPFSHLMEG
  ELEEYTKTIERKQQGLDENHELFSKSFTSVDVPVIVNGKDEMHDVEDELAQRVSRLTTSTTIENIEITIK
  SPDRTEEVLSPDGSPSKSPSKKKKKFRTPSFLKKNKKKEKVEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031552
• ORF Size: 2019 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031552.2, NP_113740.2
• RefSeq Size: 4098 bp
• RefSeq ORF: 2022 bp
• Locus ID: 25230
• UniProt ID: Q62847
• Cytogenetics: 1q55
• MW: 75.4 kDa
• Gene Summary: binds phosphatidylserine, binds and is phosphorylated by protein kinase C [RGD, Feb 2006]