Taf15 (NM_001105824) Rat Tagged ORF Clone

CAT#: RR207757

Taf15 (Myc-DDK-tagged ORF) - Rat TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor (Taf15), (10 ug)

Product Images

Circular map for RR207757

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Taf15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207757 representing NM_001105824
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAATCAAGGCAGTCAAGGCTATGGACAGACACAACAAAGCTATTCTGGCTATGGGCAAACGACTGA
  TTCTTCATATGGACAAAACTATGGTGGTTACTCCGGTTATGGACAAAATCAATCAGGTTATTCACAGTCC
  TATGGTAGTTATGAGAATCAAAAGCAGAGTTCCTATGGCCAGCAGTCATATAATAACCAGGGACAACAAA
  ACACGGAATCATCAGGAGGTCAAGGTGGAAGAGCACCTTCATATGGCCAGTCAGACTATGGTCAGCAAGA
  TTCTTATGACCAGCAGTCAGGTTATGATCAGCACCAAGGCTCATATGATGAGCAGTCCAATTATCAGCAG
  CATGATTCCTATAATCAAAACCAGCAGTCTTACCATCCACAAAGGGAAAACTATAGCCACCACACACAAG
  TGCAGTGGTGGTGACCGCGGTGGCTTCAAAAATTTTGGTGGTCACAGGGATTATGGACCCAGACCAGATG
  CTGATTCAGAGTCTGATAATTCAGATAACAACACAATCTTTGTGCAAGGACTTGGGGAGGGTGTGTCTAC
  AGATCAAGTGGGAGAGTTCTTTAAGCAAATAGGAATTATCAAGACTAACAAAAAGACTGGAAAACCAATG
  ATAAATCTTTACACAGACAAAGACACAGGAAAGCCAAAAGGAGAAGCAACAGTGTCATTTGATGACCCTC
  CATCAGCTAAGGCAGCCATTGATTGGTTTGATGGAAAAGAATTCCATGGAAACATCATTAAAGTGTCATT
  TGCCACGAGAAGGCCTGAATTCATGAGAGGCGGTGGAAGTGGAGGTGGACGGCGAGGTCGTGGAGGATAT
  AGAGGCCGTGGAGGTTTTCAGGGGCGAGGTGGAGACCCCAAAAATGGGGACTGGGTTTGCCCCAATCCGT
  CATGTGGGAATATGAATTTTGCTCGAAGGAATTCCTGTAATCAGTGCAATGAGCCAAGACCAGAGGACTC
  TCGTCCCTCAGGGGGAGAAATGACTACAGAAATGATCAGCGCAACCGACCGTACTGATGACTGTTTCCAA
  TACTCCCTTGTCTCTGACATGATCCATGGGGAATTGTCAGAGTGTGCCTGCTGCTTTCCTCATGGCCTCT
  TGAGTAGTGAAATTGACATTTGGGTTTTTATTTGGGTGGGAGGGCTGGGGACAGTTTTCCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207757 representing NM_001105824
  Red=Cloning site Green=Tags(s)
  
  MEIKAVKAMDRHNKAILAMGKRLILHMDKTMVVTPVMDKINQVIHSPMVVMRIKSRVPMASSHIITRDNK
  TRNHQEVKVEEHLHMASQTMVSKILMTSSQVMISTKAHMMSSPIISSMIPIIKTSSLTIHKGKTIATTHK
  CSGGDRGGFKNFGGHRDYGPRPDADSESDNSDNNTIFVQGLGEGVSTDQVGEFFKQIGIIKTNKKTGKPM
  INLYTDKDTGKPKGEATVSFDDPPSAKAAIDWFDGKEFHGNIIKVSFATRRPEFMRGGGSGGGRRGRGGY
  RGRGGFQGRGGDPKNGDWVCPNPSCGNMNFARRNSCNQCNEPRPEDSRPSGGEMTTEMISATDRTDDCFQ
  YSLVSDMIHGELSECACCFPHGLLSSEIDIWVFIWVGGLGTVFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001105824
• ORF Size: 1182 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001105824.1, NP_001099294.1
• RefSeq Size: 1494 bp
• RefSeq ORF: 1185 bp
• Locus ID: 287571
• Cytogenetics: 10q26
• MW: 43.2 kDa