Cdca5 (NM_001115042) Rat Tagged ORF Clone

CAT#: RR207724

Cdca5 (Myc-DDK-tagged ORF) - Rat similar to cell division cycle associated 5 (LOC684771), (10 ug)

Product Images

Circular map for RR207724

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cdca5
• Synonyms: RGD1560863
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207724 representing NM_001115042
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGAGCGGCGAACACGATCCGGAGGGGCCGTCCAGCGGTCTGGGCCCAGAACTTCACTTACTAAGC
  CTTCAAAGTCGTCCAAGCGGAAATCTGGCTCAGATCTCCCGAACACGTTCCCTGAAGTTTGGCCGAAGAC
  AACTCCAGCAGCTCCAGTCAGAAAAGCCATCATCTTGAAGAAGATTGTACCTCGTGCAGTAGAGGTCCCA
  GATGTCCACACACCAGTTCGCAGGAGTCCTAGGATCTCTTTTATCTTGGAAAAAGAGAACAACCCTCCAC
  TCAAGGTGCCCACTAAGGAGGACCTCTTCAAGACGTGTAGCGTCCCTGGCACTCCCTCCAGCACCCCCGT
  GCTGTGCGCTCAGAATGTTAAGTCTAACTCTAGAGAAGTGGAGCCGGACACCAGAGATTTGGAAATGTCT
  CAGAAAGTCAGGCGGTCCTACAGTCGGCTACAGAGCCTCCATTGTGCCTCAACCTCCACCCCCGGCCGCC
  GATCCTTCTTTGGCTTTGAGAGACCTGAAGACTTGCCTGGAGTCTCACCTGTCATTCGTTCAAAGTTAAT
  GGAGACCCCAAAGGTGCCTGTGAAACACTTGGTTCCAGATAGAACGCTCCCTGGAATCTCTCCCCCAATT
  GTGAAAGAGAAACGAAAGAAGAAGGTTCCTGAGATCACGTCTTCCACAGCCAAGAAGATCGCCCTCTACA
  AGCAGATTTTGAAAACAGCCATGCAGCCTACTGAGCCACAACTAGAAGAACCTAAGGACTCCGACGTGGA
  GCCATCTTCAGAGCAAATGTTGAACGAGCAGCCGGAACCCACTGTCACATTCCTGTTCACACTCCTGAAC
  ACTCCTGAGCCCAGAGATGAAGAAACGGACACAGAGATATTGGAGAATCAGTTCGTGGATCAGGATGACT
  GGGGCCCTAGGCGGACCTCAGAAGAAATACAGCTTTTGCAGATGGACTGCAATAGGCTTCAGGAAGCTCT
  GTACGCTAACCAGAGGGAAAACCTGATCCTTGAAGAGAAGCTAGAAAACCTGCCTACCTTGTTGTACGGG
  GACCTGGAGAACATTTTGATCAGTCCAGAGGAATCTAGGGTCACCCAGGACACAGATAAAAACACCCAAG
  GCCAAGCAGAGGACATCTATGAGGAAGCAGAGGACATCCATGAGGAAGCAGAGGACATCCAGGAGGAAGC
  AGGGGTTACCCAGGAGGAAGTCCAACCTCTTGCACAACCAAAGAGTGTTTCTGGCCCGCGCTACTCCTTT
  GATGTTTCCATACCTTTGCCAGCATCCAGCGTATTCCCAGAAGATTCATACAGGAATGCCCCACCAGTCA
  GCCCCTCTTTAAGCCCCAGCAATAAAGATCCCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207724 representing NM_001115042
  Red=Cloning site Green=Tags(s)
  
  MSERRTRSGGAVQRSGPRTSLTKPSKSSKRKSGSDLPNTFPEVWPKTTPAAPVRKAIILKKIVPRAVEVP
  DVHTPVRRSPRISFILEKENNPPLKVPTKEDLFKTCSVPGTPSSTPVLCAQNVKSNSREVEPDTRDLEMS
  QKVRRSYSRLQSLHCASTSTPGRRSFFGFERPEDLPGVSPVIRSKLMETPKVPVKHLVPDRTLPGISPPI
  VKEKRKKKVPEITSSTAKKIALYKQILKTAMQPTEPQLEEPKDSDVEPSSEQMLNEQPEPTVTFLFTLLN
  TPEPRDEETDTEILENQFVDQDDWGPRRTSEEIQLLQMDCNRLQEALYANQRENLILEEKLENLPTLLYG
  DLENILISPEESRVTQDTDKNTQGQAEDIYEEAEDIHEEAEDIQEEAGVTQEEVQPLAQPKSVSGPRYSF
  DVSIPLPASSVFPEDSYRNAPPVSPSLSPSNKDPH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001115042
• ORF Size: 1365 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001115042.2, NP_001108514.2
• RefSeq Size: 1508 bp
• RefSeq ORF: 1368 bp
• Locus ID: 684771
• Cytogenetics: 1q43
• MW: 51.1 kDa