Slc38a9 (NM_001035251) Rat Tagged ORF Clone

SKU RR207681

Slc38a9 (Myc-DDK-tagged ORF) - Rat solute carrier family 38, member 9 (Slc38a9), (10 ug)

Product Data

• Type: Rat Tagged ORF Clone
• Target Symbol: Slc38a9
• Synonyms: RGD1311881
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207681 representing NM_001035251; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCAAACGTGGATAGTGACTCTAGGCATCTTATCTCTGAGGTAGAACATGAAGTGAATCCCGGACCTA; TGAATATCCAGTTTGACTCCTCAGACCTAAGATCTAAAAGGCCTTTCTATATAGAGCCTACCAACATCGT; CAATGTGAATGATGTCATTCAGAAGGTCAGCGACCATGCTGCTGCTATGAACAAGCGGATTCATTACTAC; AGCCGGCTCACCACTCCTGCAGACAAGGCACTGATTGCTCCAGACCACGTAGTTCCTGCTCCAGAGGAAT; GCTATGTGTACAGTCCATTGGGCTCTGCCTATAAACTTAAGAGTTACACTGAAGGCTATCGTAAAAACAC; CAGCTTAGTTACTATTTTTATGATCTGGAATACCATGATGGGGACATCTATACTAAGTATTCCTTGGGGC; ATAAAACAGGCTGGATTTACTACTGGGATGTGTGTCATCGTGCTGATGGGCCTTCTAACACTTTACTGCT; GCTATAGAGTGGTGAAGTCCCGGAGTATGATAGTTACATCGGACACAACTACCTGGGAATATCCAGATGT; CTGCAAACATTACTTTGGCTCTTTTGGGCAATGGTCAAGTCTCCTTTTCTCCTTGGTGTCTCTCATTGGA; GCAATGATAGTTTATTGGGTGCTTATGTCTAATTTTCTTTTTAATACTGGAAAGTTTATTTTTAATTTTA; TTCATCACATTAATGACACAGACACTGTACTGAGCACCAATAATAGCAGCCCTGTGATCTGTCCAAGCGC; CGGGAGTGGCCATCCTGACAACAGCTCCATGATCTTCTACAACAGTGACACGGAGGTGCGGCTGTTTGAG; AGGTGGTGGGACAAGTCCAAGACAGTGCCCTTCTACCTTATAGGGCTCCTCTTGCCGCTGCTCAACTTCA; AGTCTCCTTCGTTCTTCTCCAAATTTAATATCCTGGGTACGGTATCTGTCCTCTATTTGATTTTCATTGT; TACCCTGAAGGCTATTCGCTTAGGATTCCATCTGGAATTTCACTGGTTTGCGCCAACAGAATTTTTTGTA; CCAGAGATAAGAGCTCAGTTTCCACAGCTGACAGGGGTGCTTACTCTGGCCTTCTTTATTCACAATTGTA; TCATCACACTCTTGAAAAACAACAAGAACCAAGAAAATAATGTGAGAGACCTGTGCATTGCTTATATGCT; GGTGACATTGACCTATCTCTATATTGGTGTCCTGGTTTTTGCCTCATTCCCTTCACCTCCATTACCCAAA; GATTGTATTGAACAGAACTTCTTGGACAACTTCCCCAGCAGTGACACCCTGTCCTTCATTGCACGGATAT; GCCTGCTCTTCCAGATGATGACTGTGTACCCACTCTTAGGCTACTTGGCTCGCGTTCAGCTCCTGGGCCA; TATCTTCGGTGACATTTATCCTAGCATTTTCCATGTGCTGATTCTTAACTTAATTATCGTGGGTGCTGGA; GTGACCATGGCCTGTTTCTACCCAAACATAGGAGGGATCATAAGGTACTCGGGAGCAGCGTGCGGCCTGG; CTTTCGTGTTCATATACCCTTCTCTCATCTATATACTCTCCCAGCACCAAGAGGAGCGTCTGACATGGCC; TAAGTTAGTGTTTCACATCATCATCATCATTTTGGGCCTGGCGAACCTGATTGCTCAGTTTTTCATG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207681 representing NM_001035251; Red=Cloning site Green=Tags(s); ; MANVDSDSRHLISEVEHEVNPGPMNIQFDSSDLRSKRPFYIEPTNIVNVNDVIQKVSDHAAAMNKRIHYY; SRLTTPADKALIAPDHVVPAPEECYVYSPLGSAYKLKSYTEGYRKNTSLVTIFMIWNTMMGTSILSIPWG; IKQAGFTTGMCVIVLMGLLTLYCCYRVVKSRSMIVTSDTTTWEYPDVCKHYFGSFGQWSSLLFSLVSLIG; AMIVYWVLMSNFLFNTGKFIFNFIHHINDTDTVLSTNNSSPVICPSAGSGHPDNSSMIFYNSDTEVRLFE; RWWDKSKTVPFYLIGLLLPLLNFKSPSFFSKFNILGTVSVLYLIFIVTLKAIRLGFHLEFHWFAPTEFFV; PEIRAQFPQLTGVLTLAFFIHNCIITLLKNNKNQENNVRDLCIAYMLVTLTYLYIGVLVFASFPSPPLPK; DCIEQNFLDNFPSSDTLSFIARICLLFQMMTVYPLLGYLARVQLLGHIFGDIYPSIFHVLILNLIIVGAG; VTMACFYPNIGGIIRYSGAACGLAFVFIYPSLIYILSQHQEERLTWPKLVFHIIIIILGLANLIAQFFM; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001035251
• ORF Size: 1677 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001035251.1, NP_001030328.1
• RefSeq Size: 1807 bp
• RefSeq ORF: 1680 bp
• Locus ID: 310091
• UniProt ID: Q3B8Q3
• Cytogenetics: 2q14
• MW: 63.4 kDa
• Summary: Lysosomal amino acid transporter involved in the activation of mTORC1 in response to amino acid levels. Probably acts as an amino acid sensor of the Rag GTPases and Ragulator complexes, 2 complexes involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Following activation by amino acids, the Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. SLC38A9 mediates transport of amino acids with low capacity and specificity with a slight preference for polar amino acids. Acts as an arginine sensor. Following activation by arginine binding, mediates transport of leucine, tyrosine and phenylalanine with high efficiency, and is required for the efficient utilization of these amino acids after lysosomal protein degradation.[UniProtKB/Swiss-Prot Function]