Cyb5r4 (NM_133427) Rat Tagged ORF Clone

CAT#: RR207642

Cyb5r4 (Myc-DDK-tagged ORF) - Rat cytochrome b5 reductase 4 (Cyb5r4), (10 ug)

Product Images

Circular map for RR207642

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyb5r4
• Synonyms: b5&b5R; b5+b5R; cb5/cb5R; Ncb5or
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207642 representing NM_133427
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAACGTCCCTTCCCAGGCTTTCCCGGCCCCCGGCTCCCAGCAGCGCGTTGCCTCCCAGGGGCGTA
  GCAAGGTACCTTTAAAACAAGGCAGGAGTCTTATGGATTGGATCCGATTGACCAAAAGTGGAAAGGACTT
  CACGGGATTGAAGGGCGGGTTAATTGAAGTAACAGAAGAAGAACTTAAGAAGCACAACAAGAAAGACGAC
  TGTTGGATATGCATAAGAGGGTTTGTTTACAATGTCAGCCCATATATGGAGTATCATCCTGGTGGGGAGG
  ATGAGCTTATGAGAGCCGCAGGAGCAGATGGAACGGATCTTTTTAATGAGGTGCATCGTTGGGTCAATTA
  TGAATCCATGCTGAAAGAGTGCCTGGTTGGCAGGATGGCTGTGAAGCCCGCTGTTCCGAAAGACTGTCAT
  GAAGGAAAGAGAGTCTTAAATGGCATGCTTCCCAAGAGCCAAGTGACAGACACACTGCCCAGGGAAGGCC
  CCAGTTCTCCAAGCTATGATTGGTTCCAAACAGAGTCTTCAGTGACCATTGTTATATACACCAAGCAGAA
  GAATATCAATTTAGACTCAGTAATCGTTGACCTCCAGGATGACTCCTTGAGAGCAGAAGCAGTCATCAAG
  GATCATTCTTATCTTATACATATTGGGTTAAGCCATGAAGTTCAAGAAAATTTTTCTGTACGGGTTATTG
  AGAATGTTGGAAAAATAGAGATTGTCCTACAAAAGAAAGAAACTGTTTCTTGGAAATGTCTTGGTGATCC
  CCTGGAGAAACATGATTCTTTTATTCCAAAGAAAGATACAGGCTTGTACTATAGACAGTGCCAGTTAATT
  TCCAAGGAAGACGTTACTCATGACACAAGGCTCTTCTGTCTGATGCTGCCTCCCAGTACTCACCTTCAGG
  TGCCAGTTGGGCAACATGTTTACCTCAAGCTCAGTGTCACAGGTGCAGAAATAGTAAAGCCATATACACC
  CGTGTCTGAGTCCTTACTCTCAGATTTTAAAGAACCAGTTCTTTCCCCAAATAAGTACATCTACTTTTTG
  ATCAAAATCTATCCTGCTGGACTCTTCACGCCAGAACTTGATCGTCTTCAGATTGGAGATTTTGTTTCTG
  TAAGTGGTCCTGAGGGCAATTTTAAAGTTTCCAAACTCCAGGAAGTGGAAGATCTCTTTTTATTGGCAGC
  CGGAACAGGCTTCACACCAATGGTTACTGTACTGAATCACGCTTTAACTCACATGTCCAGTCTCAGGAAG
  GTGAAGTTAATGTTCTTCAATAAAACAGAGGATGACATAATTTGGAGATGCCAGCTAGAGAAATTGGCAT
  TAAAAGATAAAAGATTTCATGTTGAATATGTTCTCTCGGCACCTTCCCCAGAATGGAATGGCAAACAGGG
  ACACGTGTCACGAGCTCTTCTCTCTGAATTTTTGCAACGAAGTTTAGAAAATTCTAAAGTCTTCCTTTGC
  ATCTGTGGACCAACCCCATTTACAGATGAGGGGATAAGGTTGCTGCATGATCTTAACTTTTCCGATGATG
  AGATTCATGGTTTTACAGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207642 representing NM_133427
  Red=Cloning site Green=Tags(s)
  
  MLNVPSQAFPAPGSQQRVASQGRSKVPLKQGRSLMDWIRLTKSGKDFTGLKGGLIEVTEEELKKHNKKDD
  CWICIRGFVYNVSPYMEYHPGGEDELMRAAGADGTDLFNEVHRWVNYESMLKECLVGRMAVKPAVPKDCH
  EGKRVLNGMLPKSQVTDTLPREGPSSPSYDWFQTESSVTIVIYTKQKNINLDSVIVDLQDDSLRAEAVIK
  DHSYLIHIGLSHEVQENFSVRVIENVGKIEIVLQKKETVSWKCLGDPLEKHDSFIPKKDTGLYYRQCQLI
  SKEDVTHDTRLFCLMLPPSTHLQVPVGQHVYLKLSVTGAEIVKPYTPVSESLLSDFKEPVLSPNKYIYFL
  IKIYPAGLFTPELDRLQIGDFVSVSGPEGNFKVSKLQEVEDLFLLAAGTGFTPMVTVLNHALTHMSSLRK
  VKLMFFNKTEDDIIWRCQLEKLALKDKRFHVEYVLSAPSPEWNGKQGHVSRALLSEFLQRSLENSKVFLC
  ICGPTPFTDEGIRLLHDLNFSDDEIHGFTA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133427
• ORF Size: 1560 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133427.3, NP_596918.3
• RefSeq Size: 2252 bp
• RefSeq ORF: 1563 bp
• Locus ID: 171015
• UniProt ID: Q68EJ0
• Cytogenetics: 8q31
• MW: 58.8 kDa
• Gene Summary: NADH-cytochrome b5 reductase involved in endoplasmic reticulum stress response pathway. Plays a critical role in protecting pancreatic beta-cells against oxidant stress, possibly by protecting the cell from excess buildup of reactive oxygen species (ROS).[UniProtKB/Swiss-Prot Function]