Asic1 (NM_024154) Rat Tagged ORF Clone

CAT#: RR207637

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  • TrueORF®

Asic1 (Myc-DDK-tagged ORF) - Rat amiloride-sensitive cation channel 2, neuronal (Accn2), (10 ug)



  "NM_024154" in other vectors (3)

USD 710.00


Availability*
5 Weeks

Size
    • 10 ug


Product images

Specifications

Product Data
Product Name Asic1 (NM_024154) Rat Tagged ORF Clone
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Asic1
Synonyms Accn2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RR207637 representing NM_024154
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAATTGAAGACCGAGGAGGAGGAGGTGGGTGGTGTCCAGCCGGTGAGCATCCAGGCTTTCGCCAGCA
GCTCCACGCTGCATGGTCTTGCCCACATCTTCTCCTATGAGCGGCTGTCTCTGAAGCGGGCACTGTGGGC
CCTGTGCTTCCTGGGTTCGCTGGCCGTCCTGCTGTGTGTGTGCACTGAGCGTGTGCAGTACTACTTCTGC
TATCACCACGTCACCAAGCTTGACGAAGTGGCTGCCTCCCAGCTCACCTTCCCTGCTGTCACACTGTGCA
ATCTCAATGAGTTCCGCTTTAGCCAAGTCTCCAAGAATGACCTGTACCATGCTGGGGAGCTGCTGGCCCT
GCTCAACAACAGGTATGAGATCCCGGACACACAGATGGCTGATGAAAAGCAGCTAGAGATATTGCAGGAC
AAGGCCAACTTCCGGAGCTTCAAGCCCAAGCCCTTCAACATGCGTGAATTCTACGACAGAGCGGGGCACG
ATATTCGAGACATGCTGCTCTCGTGCCACTTCCGTGGGGAGGCCTGCAGCGCTGAAGATTTCAAAGTGGT
CTTCACTCGGTATGGGAAGTGTTACACATTCAACTCGGGCCAAGATGGGCGGCCACGGCTGAAGACCATG
AAAGGTGGGACTGGCAATGGCCTGGAGATCATGCTGGACATTCAGCAAGATGAATATTTGCCTGTGTGGG
GAGAGACCGACGAGACATCCTTCGAAGCAGGCATCAAAGTGCAGATCCACAGTCAGGATGAACCCCCTTT
CATCGACCAGCTGGGCTTTGGTGTGGCTCCAGGTTTCCAGACGTTTGTGTCTTGCCAGGAGCAGAGGCTC
ATCTACCTGCCCTCACCCTGGGGCACCTGCAATGCTGTTACCATGGACTCGGATTTCTTCGACTCCTACA
GCATCACTGCCTGCCGGATTGATTGCGAGACGCGTTACCTGGTGGAGAACTGCAACTGCCGTATGGTGCA
CATGCCAGGGGACGCCCCATACTGCACTCCAGAGCAGTACAAGGAGTGTGCAGATCCTGCCCTGGACTTC
CTAGTGGAGAAAGACCAGGAATACTGCGTGTGTGAGATGCCTTGCAACCTGACCCGCTACGGCAAGGAGC
TGTCCATGGTCAAGATCCCAAGCAAAGCCTCCGCCAAGTACCTGGCCAAGAAGTTCAACAAATCGGAGCA
GTACATAGGGGAGAACATTCTGGTGCTGGACATTTTCTTTGAAGTCCTCAACTATGAGACCATCGAGCAG
AAAAAGGCCTATGAGATCGCAGGGCTGTTGGGTGACATCGGGGGCCAGATGGGGTTGTTCATCGGTGCCA
GCATCCTCACCGTGCTGGAACTCTTTGACTATGCCTACGAGGTCATTAAGCACAGGCTGTGCAGACGTGG
AAAGTGCCAGAAGGAGGCTAAGAGGAGCAGCGCAGACAAGGGCGTGGCGCTCAGCCTGGATGACGTCAAA
AGACACAATCCCTGCGAGAGCCTCCGAGGACATCCTGCCGGGATGACGTACGCTGCCAACATCCTACCTC
ACCATCCCGCTCGAGGCACGTTTGAGGACTTTACCTGC


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>RR207637 representing NM_024154
Red=Cloning site Green=Tags(s)

MELKTEEEEVGGVQPVSIQAFASSSTLHGLAHIFSYERLSLKRALWALCFLGSLAVLLCVCTERVQYYFC
YHHVTKLDEVAASQLTFPAVTLCNLNEFRFSQVSKNDLYHAGELLALLNNRYEIPDTQMADEKQLEILQD
KANFRSFKPKPFNMREFYDRAGHDIRDMLLSCHFRGEACSAEDFKVVFTRYGKCYTFNSGQDGRPRLKTM
KGGTGNGLEIMLDIQQDEYLPVWGETDETSFEAGIKVQIHSQDEPPFIDQLGFGVAPGFQTFVSCQEQRL
IYLPSPWGTCNAVTMDSDFFDSYSITACRIDCETRYLVENCNCRMVHMPGDAPYCTPEQYKECADPALDF
LVEKDQEYCVCEMPCNLTRYGKELSMVKIPSKASAKYLAKKFNKSEQYIGENILVLDIFFEVLNYETIEQ
KKAYEIAGLLGDIGGQMGLFIGASILTVLELFDYAYEVIKHRLCRRGKCQKEAKRSSADKGVALSLDDVK
RHNPCESLRGHPAGMTYAANILPHHPARGTFEDFTC

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_024154
ORF Size 1578 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_024154.1, NM_024154.2, NP_077068.1
RefSeq Size 3771 bp
RefSeq ORF 1581 bp
Locus ID 79123
Cytogenetics 7q36
MW 59.6 kDa
Gene Summary forms a proton-gated ion channel that may play a role in sensory neuron function and pain perception [RGD, Feb 2006]

Citations (1)

Other Versions

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Frequently bought together (3)
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Customer Reviews 
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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