Atpaf1 (NM_001107959) Rat Tagged ORF Clone

CAT#: RR207398

Atpaf1 (Myc-DDK-tagged ORF) - Rat ATP synthase mitochondrial F1 complex assembly factor 1 (Atpaf1), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR207398

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atpaf1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207398 representing NM_001107959
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAGGCGCGCGGGCCGGGGGCCGAGGCTGACGCGCCAGAGGAGGAGGAGGGGGAGGCGCAGGAGG
  CCATGGCGGCTGTAGTGTCCGCTGCGGGTGGAGCGTGCCCCGCAGTCCTGCAGGTGGCCGGCCTCTACCG
  AGGCTTGTGTGCGGTGCGCAGCCGCGCCCTGGGGCTGGGGTTCGTGTCACCTGCACAGTTGCGCGTGTTC
  CCGGTGCGCCGGGGCTCGGGCCTGTCCTCCGGGGGAACAGACGGCGGTGGGGTCAGCGAGCTGGAAACTA
  ACCCTTTCTACGACCGCTACCGCGACAAGATCCAGCAGCTGCGCAGGTCTGACCCAGCTGCCTTTGAGTC
  CCGCCTGGAGAAGCGCAGTGAGTTCCGAAAGCAGCCAGTGGGGCACTCCAAACAAAGTGATTTTATCAGG
  TGTGTGGAGCAGAAGACAGATGCCTTGGGGAAACAGCCTGTGAGCAAAGGATTCACTAAGGACAAGACTC
  TCAGTTCAGTCTTCAATGTTGAGATGGTGAAGGACAAAACCGGAGAGGAAATAAAACAGATTTGGCAGCA
  GTATTTTTCAGCAAAAGACACTGTCTACGCAGTTATTCCTAAAGAGAAGTTTGATGTGATCTGGAACCGG
  GCTCAGTCCTGCCCAACATTTCTTTGTGCACTACCAAGAAGGGATGGTTATGAGTTCTTTGTAGGACAAT
  GGACAGGTACAGAGCTCCACTTCACTGCACTTATAAATATTCAGACCCGAGGGGATGCAGCAGCCAGCCA
  GCTGATTTTATACCACTATCCTGAACTTAAGGAAGAAAAGGGCATAGTGCTGATGACAGCAGAAATGGAT
  TCCACATTTCTGAACGTTGCCGAGGCGCAGTGCATCGCCAATCAGGTTCAGCTCTTCTACGCCACTGATC
  GGAAGGAGATCTATGGGCTAGTAGAGACCTTTAACTTCAGACCAAATGAGTTCAAATATATGTCTGTCAT
  CGCTGAATTGGAGCAAAGTGGACTTGGAGCAGAACTGAAATGTGCCCAGACCCAAGATAAGACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207398 representing NM_001107959
  Red=Cloning site Green=Tags(s)
  
  MEQARGPGAEADAPEEEEGEAQEAMAAVVSAAGGACPAVLQVAGLYRGLCAVRSRALGLGFVSPAQLRVF
  PVRRGSGLSSGGTDGGGVSELETNPFYDRYRDKIQQLRRSDPAAFESRLEKRSEFRKQPVGHSKQSDFIR
  CVEQKTDALGKQPVSKGFTKDKTLSSVFNVEMVKDKTGEEIKQIWQQYFSAKDTVYAVIPKEKFDVIWNR
  AQSCPTFLCALPRRDGYEFFVGQWTGTELHFTALINIQTRGDAAASQLILYHYPELKEEKGIVLMTAEMD
  STFLNVAEAQCIANQVQLFYATDRKEIYGLVETFNFRPNEFKYMSVIAELEQSGLGAELKCAQTQDKT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107959
• ORF Size: 1044 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107959.1, NP_001101429.1
• RefSeq Size: 3066 bp
• RefSeq ORF: 1047 bp
• Locus ID: 313510
• Cytogenetics: 5q35
• MW: 38.6 kDa