Ldha (NM_017025) Rat Tagged ORF Clone

CAT#: RR207357

Ldha (Myc-DDK-tagged ORF) - Rat lactate dehydrogenase A (Ldha), (10 ug)

Product Images

Circular map for RR207357

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ldha
• Synonyms: CDK1; Ldh1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >NM_017025 ORF sequence, RR207357 may differ due to SNPs.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCAGCCCTCAAGGACCAGCTGATTGTGAATCTTCTTAAGGAAGAACAGGTCCCCCAGAACAAGATT
  ACAGTTGTTGGGGTTGGTGCTGTTGGCATGGCTTGTGCCATCAGTATCTTAATGAAGGACTTGGCTGAT
  GAGCTTGCCCTTGTTGATGTCATAGAAGATAAGCTAAAGGGAGAGATGATGGATCTTCAGCATGGCAGC
  CTTTTCCTTAAGACACCAAAAATTGTCTCCAGCAAAGATTATAGTGTGACTGCAAACTCCAAGCTGGTC
  ATTATCACCGCGGGGGCCCGTCAGCAAGAGGGAGAGAGCCGGCTCAATTTGGTCCAGCGAAACGTGAAC
  ATCTTCAAGTTCATCATTCCAAATGTTGTGAAATACAGTCCACAGTGCAAACTGCTCATCGTCTCAAAC
  CCAGTGGATATCTTGACCTACGTGGCTTGGAAGATCAGCGGCTTCCCCAAAAACAGAGTTATTGGAAGT
  GGTTGCAATCTGGATTCGGCTCGGTTCCGTTACCTGATGGGAGAAAGGCTGGGAGTTCATCCACTGAGC
  TGTCACGGGTGGGTCCTGGGAGAGCATGGCGACTCCAGTGTGCCTGTGTGGAGTGGTGTGAATGTCGCC
  GGCGTCTCCCTGAAGTCTCTGAACCCGCAGCTGGGCACGGATGCAGACAAGGAGCAGTGGAAGGATGTG
  CACAAGCAGGTGGTTGACAGTGCATACGAAGTGATCAAGCTGAAAGGTTACACATCCTGGGCCATTGGC
  CTCTCCGTGGCAGACTTGGCCGAGAGCATAATGAAGAACCTTAGGCGGGTGCATCCCATTTCCACCATG
  ATTAAGGGTCTCTATGGAATCAAGGAGGATGTCTTCCTCAGCGTCCCATGTATCCTGGGACAAAATGGA
  ATCTCAGATGTTGTGAAGGTGACACTGACTCCTGACGAGGAGGCCCGCCTGAAGAAGAGTGCAGATACC
  CTCTGGGGAATCCAGAAGGAGCTGCAGTTC
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by RR207357
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MAALKDQLIVNLLKEEQVPQNKITVVGVGAVGMACAISILMKDLADELALVDVIEDKLKGEMMDLQHGS
  LFLKTPKIVSSKDYSVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNVVKYSPQCKLLIVSN
  PVDILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHPLSCHGWVLGEHGDSSVPVWSGVNVA
  GVSLKSLNPQLGTDADKEQWKDVHKQVVDSAYEVIKLKGYTSWAIGLSVADLAESIMKNLRRVHPISTM
  IKGLYGIKEDVFLSVPCILGQNGISDVVKVTLTPDEEARLKKSADTLWGIQKELQF
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017025
• ORF Size: 996 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 1598 bp
• RefSeq ORF: 999 bp
• Locus ID: 24533
• UniProt ID: P04642
• Cytogenetics: 1q22
• MW: 36.5 kDa
• Gene Summary: mRNA expression in fibroblasts increases in response to induction by epidermal growth factor or serum [RGD, Feb 2006]