Kcng2 (NM_001107372) Rat Tagged ORF Clone

CAT#: RR207262

Kcng2 (Myc-DDK-tagged ORF) - Rat potassium voltage-gated channel, subfamily G, member 2 (Kcng2), (10 ug)

Product Images

Circular map for RR207262

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcng2
• Synonyms: Kv6.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207262 representing NM_001107372
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGGCTCCCGGGGCACCCGGAGGTCCCCGGCGCCGAGCCTGGCAGCGCAGTCCGAGGGGGCCGAG
  GCGGCCGCGGGGCCCGGGCGCGCCACGTCATAATCAACGTCGGGGGCTGCAGGGTGCGTCTGGCCTGGGC
  TGCGCTGGCCCGCTGTCCCCTGGCTCGCCTCGAGCGCCTGCGAGCCTGCCGCGGCCACGACGAGCTGCTG
  CGCGTGTGTGACGACTACGACGTGAGCCGCGACGAGTTCTTCTTCGACCGCAGCCCGTGCGCTTTCCGCG
  CCATCGTGGCGCTGCTGCGCGCCGGCAAGCTGAGGCTGCTGCGCGGCCCGTGCGCCCTGGCCTTCCGGGA
  CGAGCTGGCCTACTGGGGCATCGACGAGGCGCGGCTGGAGCGCTGCTGCCTGCGCCGTCTGCGCCGTCGC
  GAGGAGGAGGCCGCCGAGGCGAGGGCCACGCCGCCCGCCCGAGGGCCGCAGACCAGCCCCGGCCGAGCCC
  TGGGGAGCGGGCGGCTGGAGCGAGGCCGACGGCGCCTGCGCGACGTGGTGGAGAACCCGCACTCCGGGCT
  GGCGGGCAAGCTCTTCGCCTACGTCTCCGTGGCCTTCGTGGCCGTCACGGCCGTGGGCCTGTGCCTGAGC
  ACCATGCCTGACGTCCGCGCAGAGGAGGAAAGGGGCGAGTGCTCCACCAAGTGCCGCAACCTCTTCGTGT
  TGGAGACAGTGTGCGTGGCCTGGTTCTCCTTCGAGTTCCTGCTGCGCTCCCTGCAAGCCGAGAGCAAGTG
  CGCCTTCCTCCGTACGCCTCTTGCCATCATCGACATCCTGGCCATCCTGCCCTTCTACGTGTCGCTGCTC
  GCGGGACTGGCCGCAGGGCCCACGGGCAGCAAGATGCTGGAACGCGCGGGGCTGGTACTGCGGCTACTGC
  GGGCGCTGCGCGTGCTCTACGTGATGCGCCTGGCGCGCCACTCGCTGGGGCTACGCTCGCTTGGCCTCAC
  CGTGCGCCGCTGCGCGCGCGAGTTCGGACTGCTGCTGCTCTTCCTCTGCGTGGCCATGGCGCTCTTCGCG
  CCGCTCGTGCACCTGGCTGAGCGCGAGCTGGGCGCTCACCGCGACTTCTCCAGCGTCCCCGCCAGCTACT
  GGTGGGCAGTCATCTCCATGACCACCGTGGGCTATGGAGACATGGTGCCACGCAGCCTTCCGGGCCAGGT
  GGTGGCGCTGAGCAGCATCCTCAGCGGCATCCTGCTCATGGCCTTCCCTGTCACCTCCATCTTCCACACC
  TTCTCGCGCTCCTACTCGGAGCTCAAGGAGCAGCAGCAACGCGCGGCCAGCCCTGAACCGGTCCTGCGGG
  AGGACAGCACACGCGACGACAGCACGCGTTCGGCCAGCGCCACCGAGGACAGCTCCCAGGACCCGGAGAC
  CGCAGGTGCCGCAGGGAGCTTGCCAGGCCCGGTGGGACCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207262 representing NM_001107372
  Red=Cloning site Green=Tags(s)
  
  MARLPGHPEVPGAEPGSAVRGGRGGRGARARHVIINVGGCRVRLAWAALARCPLARLERLRACRGHDELL
  RVCDDYDVSRDEFFFDRSPCAFRAIVALLRAGKLRLLRGPCALAFRDELAYWGIDEARLERCCLRRLRRR
  EEEAAEARATPPARGPQTSPGRALGSGRLERGRRRLRDVVENPHSGLAGKLFAYVSVAFVAVTAVGLCLS
  TMPDVRAEEERGECSTKCRNLFVLETVCVAWFSFEFLLRSLQAESKCAFLRTPLAIIDILAILPFYVSLL
  AGLAAGPTGSKMLERAGLVLRLLRALRVLYVMRLARHSLGLRSLGLTVRRCAREFGLLLLFLCVAMALFA
  PLVHLAERELGAHRDFSSVPASYWWAVISMTTVGYGDMVPRSLPGQVVALSSILSGILLMAFPVTSIFHT
  FSRSYSELKEQQQRAASPEPVLREDSTRDDSTRSASATEDSSQDPETAGAAGSLPGPVGP
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107372
• ORF Size: 1440 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107372.1, NP_001100842.1
• RefSeq Size: 1838 bp
• RefSeq ORF: 1443 bp
• Locus ID: 307234
• UniProt ID: Q9QYU3
• Cytogenetics: 18q12.3
• MW: 52.5 kDa
• Gene Summary: Potassium channel subunit. Modulates channel activity by shifting the threshold and the half-maximal activation to more negative values (By similarity).[UniProtKB/Swiss-Prot Function]