Sox18 (NM_001024781) Rat Tagged ORF Clone

CAT#: RR206922

Sox18 (Myc-DDK-tagged ORF) - Rat SRY (sex determining region Y)-box 18 (Sox18), (10 ug)

Product Images

Circular map for RR206922

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sox18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206922 representing NM_001024781
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAGATCGCCGCCCGGCTACGGCGCACAGGACGACCCGCCCTCCCGCCGCGACTGTGCATGGGCCC
  CCGGACTCGGGGCCGCTGCTGAGCCGCGCGGCCTCCCGGTCACCAACGTCTCGCCTACCTCGCCTGCCTC
  GCCGTCCAGCCTTCCGCGGAGCCCACCGCGCAGCCCCGAATCAGGGCGCTATGGCTTTGGCCGCGGAGAG
  CGCCAAACGGCCGACGAGTTGCGCATCCGGCGGCCCATGAACGCCTTTATGGTGTGGGCGAAGGACGAGC
  GCAAGCGACTGGCGCAACAAAATCCGGATCTCCACAACGCAGTGCTGAGCAAGATGCTGGGCAAAGCGTG
  GAAGGAGCTGAACACGGCGGAGAAGAGGCCCTTCGTGGAAGAGGCCGAACGGCTGCGTGTGCAGCACTTG
  CGCGACCATCCCAACTACAAGTACCGGCCTCGCCGCAAAAAACAGGCGCGCAAGGTCCGGAGGCTGGAGC
  CTGGCCTCTTGCTCCCGGGCCTCGTGCAGCCGTCTGCGCCGCCGGAACCCTTCGCTGCAGCATCAGGCTC
  AGCTCGCTCCTTCCGCGAGCTACCCACTCTGGGTGCGGAGTTCGACGGCTTGGGGCTACCCACGCCCGAG
  CGCTCGCCTCTGGACGGCCTGGAGTCTGGCGAGGCCTCCTTCTTCCCACCGCCTTTGGCGCCCGAGGACT
  GCGCTCTGCGGGCTTTCCGGGCGCCCTATGCCCCGGAGCTGGCACGGGACCCGAGCTTCTGCTACGGGTC
  ATCCCTGGCTGAGGCGCTCAGGACAGCGCCGCCTGCCGCGCCGCTCGCGGGCCTCTACTATGGCACCCTG
  GGCACTCCGGGCCCGTTTCCCAATCCTCTGTCACCACCACCTGAGGCCCCGTCTCTTGAGGGCACAGAGC
  AATTGGAGCCTACCGCCGACCTTTGGGCCGATGTGGACCTCACCGAATTCGATCAGTATCTCAATTGCAG
  CCGGACTCGACCGGATGCCACTGCACTCCCCTACCATGTGGCACTGGCCAAACTAGGTCCGCGTGCCATG
  TCCTGTCCAGAGGAAAGCAGCCTCATTTCTGCACTGTCTGACGCCAGCAGCGCGGTCTATTACAGCGCTT
  GCATCTCAGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206922 representing NM_001024781
  Red=Cloning site Green=Tags(s)
  
  MQRSPPGYGAQDDPPSRRDCAWAPGLGAAAEPRGLPVTNVSPTSPASPSSLPRSPPRSPESGRYGFGRGE
  RQTADELRIRRPMNAFMVWAKDERKRLAQQNPDLHNAVLSKMLGKAWKELNTAEKRPFVEEAERLRVQHL
  RDHPNYKYRPRRKKQARKVRRLEPGLLLPGLVQPSAPPEPFAAASGSARSFRELPTLGAEFDGLGLPTPE
  RSPLDGLESGEASFFPPPLAPEDCALRAFRAPYAPELARDPSFCYGSSLAEALRTAPPAAPLAGLYYGTL
  GTPGPFPNPLSPPPEAPSLEGTEQLEPTADLWADVDLTEFDQYLNCSRTRPDATALPYHVALAKLGPRAM
  SCPEESSLISALSDASSAVYYSACISG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024781
• ORF Size: 1131 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024781.1, NP_001019952.1
• RefSeq Size: 1643 bp
• RefSeq ORF: 1134 bp
• Locus ID: 311723
• Cytogenetics: 3q43
• MW: 40.9 kDa