Map2 (NM_013066) Rat Tagged ORF Clone

CAT#: RR206782

Map2 (Myc-DDK-tagged ORF) - Rat microtubule-associated protein 2 (Map2), (10 ug)

Product Images

Circular map for RR206782

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Map2
• Synonyms: MAP2R; Mtap2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206782 representing NM_013066
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGACGAGAGGAAAGACGAAGGAAAGGCACCACACTGGACATCAGCCTCACTCACAGAGGCAGCTG
  CACACCCCCACTCGCCAGAGATGAAGGACCAGGGTGGCTCAGGGGAAGGGCTGAGCCGCAGCGCCAATGG
  ATTTCCATACAGAGAGGAGGAGGAAGGCGCCTTTGGGGAGCACGGGTCACAGGGCACCTATTCAGATACC
  AAAGAGAACGGGATCAACGGAGAGCTGACCTCAGCTGACAGAGAAACAGCAGAGGAAGTGTCTGCAAGGA
  TAGTTCAAGTAGTCACAGCTGAAGCTGTAGCAGTCCTGAAAGGTGAACAAGAGAAGGAGGCCCAACACAA
  GGATCAGCCTGCAGCTCTGCCTTTAGCAGCTGAAGAAACAGTTAATCTGCCACCTTCCCCACCACCATCG
  CCAGCATCAGAACAAACAGCTGCACTGGAAGAAGCCTCGAAGATGGAATTCCCTGAGCAGCAGAAATTGC
  CTTCCTCATTCGCTGAGCCTTTAGACAAGGAGGAAACGGAGTTTAAGATGCAAAGTAAGCCTGGTGAAGA
  CTTTGAACATGCTGCCTTAGTTCCTCAGCCGGACACAAGTAAAACTCCCCAGGATAAAAAGGATCCCCAA
  GACATGGAAGGAGAAAAGTCGCCTGCCAGTCCATTTGCGCAGACTTTCGGTACCAACCTGGAAGACATAA
  AACAGATCACAGAACCAAGCATAACAGTACCTAGCATTGGCCTCTCCGCAGAGCCCCTAGCTCCAAAAGA
  TCAGAAAGACTGGTTCATCGAAATGCCCGTGGAATCAAAGAAGGATGAATGGGGTTTAGCTGCCCCAATA
  TCTCCTGGCCCCTTGACACCCATGAGGGAAAAAGATGTGCTGGAGGATATCCCAAGATGGGAAGGAAAGC
  AGTTTGACTCTCCCATGCCTAGCCCCTTCCACAGTGGAAGTTTCACTCTTCCCTTAGATACTGTGAAAGA
  TGAGAGAGTCACAGAAGGGTCACAACCCTTTGCCCCTGTCTTCTTCCAATCAGATGACAAAATGTCTCTG
  CAGGACACCAGTGGTTCAGCTACTTCCAAAGAGAGTTCTAAAGATGAGGAGCCACAGAAAGATAAAGCAG
  ACAAAGTGGCAGATGTTCCTGTCTCAGAAGCTACCACTGTACTGGGAGATGTTCACAGTCCAGCTGTGGA
  AGGCTTTGTCGGGGAGAACATTTCAGGAGAAGAAAAGGGTACCACAGATCAAGAGAAAAAAGAGACTTCG
  ACACCCAGTGTACAGGAACCTACACTCACTGAAACTGAACCACAGACAAAGCTTGAAGAGACATCAAAGG
  TTTCCATCGAAGAAACTGTGGCAAAAGAAGAGGAATCCTTGAAATTAAAAGATGATAAAGCAGGTGTAAT
  TCAGACTTCCACCGAGCATTCTTTCTCCAAAGAAGACCAGAAAGGCGAAGAACAGACAATCGAAGCATTA
  AAACAAGACTCCTTTCCTATAAGTCTAGAACAGGCAGTTACAGATGCAGCCATGGCCACCAAGACCTTGG
  AAAAGGTTACGTCTGAGCCAGAGGCAGTAAGTGAAAAGAGAGAAATCCAGGGACTTTTTGAAGAGGATAT
  AGCTGACAAGAGTAAGCTCGAAGGCGCTGGGTCTGCAACAGTAGCCGAGGTTGAGATGCCATTTTATGAA
  GATAAATCAGGGATGTCCAAGTACTTTGAAACATCTGCATTGAAAGAAGATGTGACCAGAAGCACTGGGT
  TGGGCAGTGATTACTACGAGCTGAGTGACTCAAGAGGAAATGCCCAGGAATCTCTTGATACTGTATCTCC
  CAAGAACCAACAAGATGAAAAGGAACTTCTGGCAAAAGCTTCCCAGCCTAGTCCTCCAGCACACGAAGCA
  GGGTACAGCACTCTTGCCCAGAGTTATACATCTGATCATCCGTCCGAGTTACCTGAAGAACCAAGTTCTC
  CTCAAGAAAGAATGTTCACTATTGACCCCAAAGTTTATGGGGAGAAAAGGGACCTTCATAGTAAGAACAA
  AGATGATCTGACACTTAGTCGAAGCTTGGGGCTGGGCGGAAGGTCTGCAATAGAACAGAGAAGCATGTCC
  ATTAACTTGCCTATGTCTTGCCTTGATTCTATTGCCCTTGGGTTTAACTTTGGCCGGGGCCATGATCTTT
  CCCCTCTGGCTTCTGATATTCTAACCAACACTAGCGGAACGATGGATGAAGGAGATGATTACCTGCCCCC
  CACCACACCTGCAGTGGAGAAGATTCCTTGCTTTCCAATAGAGAGCAAAGAGGAAGAAGATAAGACAGAG
  CAAGCAAAAGTGACTGGAGGGCAAACTACCCAAGTTGAAACATCCTCCGAGTCACCCTTCCCAGCCAAAG
  AATATTACAAAAATGGCACTGTCATGGCCCCTGACCTGCCTGAGATGCTAGATCTAGCAGGGACCAGGTC
  CAGATTAGCTTCTGTGAGTGCAGATGCTGAGGTTGCCAGGAGGAAATCAGTCCCATCGGAGGCTGTGGTT
  GCAGAGAGCAGTACTGGTTTGCCACCTGTTGCTGATGACAGCCAACCCGTAAAACCAGACAGTCAACTTG
  AAGACATGGGGTACTGTGTGTTCAACAAGTACACAGTCCCTCTCCCATCGCCAGTTCAAGACAGTGAGAA
  TTTGTCAGGAGAGAGTGGTTCGTTTTATGAAGGAACCGATGACAAAGTCCGTAGAGATTTGGCCACTGAC
  CTTTCACTAATTGAGGTAAAACTTGCAGCTGCTGGAAGAGTCAAAGATGAATTCACTGCTGAGAAAGAGG
  CATCTCCACCCTCTTCTGCTGACAAATCAGGACTGAGTAGGGAGTTTGACCAAGACAGGAAAGCTAATGA
  CAAGCTGGATACTGTCCTAGAAAAGAGCGAAGAGCATGTTGATTCAAAAGAACATGCCAAGGAGTCAGAA
  GAGGTTGGGGATAAAGTAGAGCTCTTCGGATTAGGTGTAACCTATGAGCAAACCTCTGCCAAAGAACTGA
  TAACAACTAAAGAAACAGCACCTGAGAGAGCAGAGAAAGGTCTCAGTTCAGTGCCAGAGGTAGCTGAGGT
  AGAAACAACCACAAAAGCTGACCAAGGTCTAGATGTTGCTGCCAAGAAAGATGATCAGAGTCCATTAGAT
  ATAAAAGTCAGTGACTTTGGACAGATGGCTTCTGGGATGAGTGTAGATGCTGGGAAAACCATAGAGCTTA
  AGTTCGAGGTTGATCAGCAGCTGACTCTCTCATCCGAAGCACCTCAGGAAACAGATTCATTCATGGGTAT
  TGAGTCCAGCCACGTGAAGGATGGTGCCAAAGTCAGTGAAACAGAAGTCAAAGAGAAGGTGGCAAAGCCT
  GACTTGGTGCATCAGGAGGCTGTGGACAAAGAAGAGTCCTATGAGTCTAGTGGTGAGCATGAAAGCCTCA
  CCATGGAGTCCCTGAAGCCTGATGAGGGCAAGAAAGAAACATCTCCAGAGACATCACTGATACAAGATGA
  AGTTGCCCTCAAACTGTCTGTAGAAATCCCTTGCCCACCTCCAGTTTCCGAAGCTGATTCATCCATTGAT
  GAGAAGGCGGAGGTCCAGATGGAATTTATTCAGCTGCCAAAGGAAGAGAGCACAGAGACTCCGGATATAC
  CTGCCATACCTTCTGATGTCACCCAGCCACAGCCTGAAGCAGTTGTGTCCGAACCAGCAGAGGTTCGAGG
  TGAGGAAGAAGAGATCGAAGCTGAGGGAGAATATGACAAACTGCTCTTCCGCTCAGACACCCTCCAGATC
  ACCGACCTGCTTGTTCCAGGAAGTAGGGAGGAGTTTGTGGAGACCTGCCCAGGGGAGCACAAAGGTGTGG
  TTGAGTCCGTGGTAACCATCGAGGATGATTTCATCACTGTAGTACAAACCACGACTGATGAGGGAGAGTT
  GGGATCCCACAGTGTGCGCTTTGCAGCTCCAGTTCAGCCTGAGGAAGAAAGGAGACCATACCCTCATGAT
  GAAGAGCTTGAAGTACTGATGGCAGCAGAAGCCCAGGCAGAGCCCAAGGATGGCTCTCCAGATGCTCCAG
  CTACCCCTGAGAAAGAAGAGGTTCCATTCTCAGAATATAAAACAGAAACCTACGACGATTACAAAGATGA
  GACCACCATTGATGACTCCATTATGGATGCCGACAGCCTGTGGGTGGACACTCAAGATGATGATAGAAGC
  ATCTTGACAGAGCAGTTAGAAACTATTCCTAAAGAGGAGAGAGCTGAGAAGGAAGCTCGGAGACCGTCTC
  TCGAGAAACATAGAAAAGAAAAACCTTTTAAAACTGGGAGAGGCAGAATTTCCACTCCTGAAAGAAAAGT
  AGCTAAAAAGGAACCTAGCACGGTCTCCAGGGATGAAGTGAGAAGGAAAAAAGCAGTTTATAAGAAGGCT
  GAACTTGCTAAAAAATCAGAAGTTCAGGCCCACTCTCCTTCCAGGAAACTCATTTTAAAACCTGCTATCA
  AATACACTAGACCAACTCATCTCTCCTGTGTTAAGCGGAAAACCACAGCAACAAGTGGTGAATCAGCTCA
  GGCTCCCAGTGCGTTTAAACAGGCGAAGGACAAAGTCACTGATGGAATAACCAAGAGCCCAGAAAAACGT
  TCTTCCCTCCCAAGACCTTCCTCCATCCTCCCTCCTCGCAGGGGCGTATCAGGAGACAGGGAGGAGAACT
  CGTTCTCTCTGAACAGCTCCATCTCTTCAGCACGACGGACCACCAGGTCAGAACCAATTCGCAGAGCAGG
  AAAAAGCGGCACCTCAACACCTACTACCCCTGGATCTACTGCAATCACCCCTGGCACTCCTCCAAGCTAC
  TCTTCACGTACCCCAGGCACCCCTGGAACCCCGAGCTATCCCAGGACACCAGGAACCCCCAAATTTGGCA
  TCTTGGTGCCCAGTGAGAAGAAAGTTGCCATCATTCGCACTCCTCCAAAGTCCCCAGCTACTCCCAAGCA
  GCTTCGGCTCATTAACCAACCTCTGCCAGACCTGAAGAACGTCAAGTCCAAAATCGGATCAACCGACAAC
  ATCAAATACCAGCCTAAGGGGGGTCAGGTACAAATTGTTACTAAGAAGATAGACTTAAGCCATGTGACTT
  CCAAATGTGGCTCTCTAAAGAACATCCGTCACAGGCCAGGTGGTGGACGCGTGAAGATTGAGAGTGTAAA
  GCTGGATTTCAAGGAGAAGGCCCAAGCTAAAGTTGGCTCACTTGACAATGCTCACCATGTACCTGGAGGT
  GGTAACGTGAAGATTGACAGCCAAAAGCTGAACTTCCGAGAGCATGCAAAGGCCCGCGTCGACCACGGGG
  CTGAGATCATCACACAGTCGCCAAGCAGGTCAAGCGTGGCGTCTCCCCGGCGACTCAGCAATGTCTCCTC
  TTCTGGAAGCATCAACCTGCTCGAATCCCCTCAGCTGGCCACTTTGGCTGAGGACGTCACTGCGGCGCTC
  GCTAAGCAGGGCTTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206782 representing NM_013066
  Red=Cloning site Green=Tags(s)
  
  MADERKDEGKAPHWTSASLTEAAAHPHSPEMKDQGGSGEGLSRSANGFPYREEEEGAFGEHGSQGTYSDT
  KENGINGELTSADRETAEEVSARIVQVVTAEAVAVLKGEQEKEAQHKDQPAALPLAAEETVNLPPSPPPS
  PASEQTAALEEASKMEFPEQQKLPSSFAEPLDKEETEFKMQSKPGEDFEHAALVPQPDTSKTPQDKKDPQ
  DMEGEKSPASPFAQTFGTNLEDIKQITEPSITVPSIGLSAEPLAPKDQKDWFIEMPVESKKDEWGLAAPI
  SPGPLTPMREKDVLEDIPRWEGKQFDSPMPSPFHSGSFTLPLDTVKDERVTEGSQPFAPVFFQSDDKMSL
  QDTSGSATSKESSKDEEPQKDKADKVADVPVSEATTVLGDVHSPAVEGFVGENISGEEKGTTDQEKKETS
  TPSVQEPTLTETEPQTKLEETSKVSIEETVAKEEESLKLKDDKAGVIQTSTEHSFSKEDQKGEEQTIEAL
  KQDSFPISLEQAVTDAAMATKTLEKVTSEPEAVSEKREIQGLFEEDIADKSKLEGAGSATVAEVEMPFYE
  DKSGMSKYFETSALKEDVTRSTGLGSDYYELSDSRGNAQESLDTVSPKNQQDEKELLAKASQPSPPAHEA
  GYSTLAQSYTSDHPSELPEEPSSPQERMFTIDPKVYGEKRDLHSKNKDDLTLSRSLGLGGRSAIEQRSMS
  INLPMSCLDSIALGFNFGRGHDLSPLASDILTNTSGTMDEGDDYLPPTTPAVEKIPCFPIESKEEEDKTE
  QAKVTGGQTTQVETSSESPFPAKEYYKNGTVMAPDLPEMLDLAGTRSRLASVSADAEVARRKSVPSEAVV
  AESSTGLPPVADDSQPVKPDSQLEDMGYCVFNKYTVPLPSPVQDSENLSGESGSFYEGTDDKVRRDLATD
  LSLIEVKLAAAGRVKDEFTAEKEASPPSSADKSGLSREFDQDRKANDKLDTVLEKSEEHVDSKEHAKESE
  EVGDKVELFGLGVTYEQTSAKELITTKETAPERAEKGLSSVPEVAEVETTTKADQGLDVAAKKDDQSPLD
  IKVSDFGQMASGMSVDAGKTIELKFEVDQQLTLSSEAPQETDSFMGIESSHVKDGAKVSETEVKEKVAKP
  DLVHQEAVDKEESYESSGEHESLTMESLKPDEGKKETSPETSLIQDEVALKLSVEIPCPPPVSEADSSID
  EKAEVQMEFIQLPKEESTETPDIPAIPSDVTQPQPEAVVSEPAEVRGEEEEIEAEGEYDKLLFRSDTLQI
  TDLLVPGSREEFVETCPGEHKGVVESVVTIEDDFITVVQTTTDEGELGSHSVRFAAPVQPEEERRPYPHD
  EELEVLMAAEAQAEPKDGSPDAPATPEKEEVPFSEYKTETYDDYKDETTIDDSIMDADSLWVDTQDDDRS
  ILTEQLETIPKEERAEKEARRPSLEKHRKEKPFKTGRGRISTPERKVAKKEPSTVSRDEVRRKKAVYKKA
  ELAKKSEVQAHSPSRKLILKPAIKYTRPTHLSCVKRKTTATSGESAQAPSAFKQAKDKVTDGITKSPEKR
  SSLPRPSSILPPRRGVSGDREENSFSLNSSISSARRTTRSEPIRRAGKSGTSTPTTPGSTAITPGTPPSY
  SSRTPGTPGTPSYPRTPGTPKFGILVPSEKKVAIIRTPPKSPATPKQLRLINQPLPDLKNVKSKIGSTDN
  IKYQPKGGQVQIVTKKIDLSHVTSKCGSLKNIRHRPGGGRVKIESVKLDFKEKAQAKVGSLDNAHHVPGG
  GNVKIDSQKLNFREHAKARVDHGAEIITQSPSRSSVASPRRLSNVSSSGSINLLESPQLATLAEDVTAAL
  AKQGL
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013066
• ORF Size: 5475 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013066.1, NP_037198.1
• RefSeq Size: 5736 bp
• RefSeq ORF: 5478 bp
• Locus ID: 25595
• Cytogenetics: 9q32
• MW: 198.6 kDa
• Gene Summary: regulates and organizes microtubules; may serve as a scaffolding protein for neuromodulatory activities [RGD, Feb 2006]