Tesmin (NM_001012069) Rat Tagged ORF Clone

CAT#: RR206721

Mtl5 (Myc-DDK-tagged ORF) - Rat metallothionein-like 5, testis-specific (tesmin) (Mtl5), (10 ug)

Product Images

Circular map for RR206721

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tesmin
• Synonyms: Mtl5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206721 representing NM_001012069
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGACGCGCTGCTCGGCGCCATGACTGGCCCCGAAGACGAGCTGGGCGCTGAGCTGTTCGGGTCGG
  AGCGTGCGTTCGCGGACGGGCTGGCGCTGAGCCCCGCGGGGGGTGCGGCCGATCGGGATGAGCTGCCGGT
  GCTGGCCGACGCCTACCTGGGCGCCACCGAGCCGGGGGAGCCGCTGCTGCGTGCGCTCAGCCCCCCGCCG
  GGCGCTGAGGTACCGGCCGCCTTGCTTGGCGACTTCCCGGGGCTGCCCGAGCTCCGCAGCTCAGACGACG
  CCGCTCCGCCTCCCGCCTACAGCGTGCACGTGCTGTCGTCGTTACTTCCCGGTGCTCGCGGGCCCGCGCT
  GTTGCCGCTAAGCGCCGGAGTGCGCGTGATCCCAGTAGAAATCAAAGAGGCAGGTGGCGGTGTGCCAGGC
  AGCAGCCCTGAAGATGCAGCTTTCCAGGCCCCTCTGGCTCAGGAATCCTGCTGCAAGTTCTCATCATCCC
  AGGAGGCAGAGGAGGCCTCCGGCTGCCCTCGGAAAAAAGACTCCAGCCCCATGGTGATTTGTCAGCTGAA
  AGGAGGTGCCCAGATGCTCTGCATAGACAACTGTGGTGCGAGGGAGCTCAAAGCGCTCCATCTGCTTCCT
  CAATATGATGACCAGAGCAGCTTCCCACAATCAGAGCTCCCTAAGCCAATGACAACTTTAGTGGGAAGAC
  TTCTGCCAGTACCAGCAAAGTTAAATCTCATCACACAGGTCGATAACGGAGCTCTCCCATCGGCTGTCAA
  TGGGGCTGCTTTTCCCTCCGGACCTGCTCTGCAAGGCCCACCCAAAATAGCTCTGGCTGGGTACTGTGAC
  TGCTTCTCCAGTGGGGACTTCTGCAACAGCTGCAGCTGCAACAACCTGCGCCACGAGCTCGAGCGCTTCA
  AAGCCATTAAGGCGTGTCTTGATAGAAATCCTGAGGCTTTCCAACCAAAAATGGGGAAAGGCCGGCTGGG
  AGCTGCTAAGCTTCGACACAGCAAAGGGTGCAACTGTAAGCGCTCAGGCTGCCTGAAGAACTACTGTGAG
  TGCTATGAGGCCAAAATCACGTGTTCTTCCATTTGCAAATGCATTGCTTGCAAAAACTATGAAGAAAGCC
  CAGAGCGAAAAATGCTGATGAGCACGCCCCACTACATGGAGCCTGGGGACTTTGAGAGCAGCCACCATCT
  GTCCCCAGCCAAATTCTCAGGACCTCCAAGACTGAGAAAAAATAGGCAAGCCTTCTCCTGTATCTCCTGG
  GAGGTAGTGGAGGCCACATGTGCCTGCCTGCTGGCCCAGGGTGAGGAGGCAGAGCAGGAGCGCTGCTCTC
  CAAGCTTGGCTGAACAGATGGTCCTGGAGGAGTTCGGAAGGTGCCTGTCGCAGATTCTCCACATCGAGTT
  CAAGTCCAAGGGGCTGAAAATTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206721 representing NM_001012069
  Red=Cloning site Green=Tags(s)
  
  MEDALLGAMTGPEDELGAELFGSERAFADGLALSPAGGAADRDELPVLADAYLGATEPGEPLLRALSPPP
  GAEVPAALLGDFPGLPELRSSDDAAPPPAYSVHVLSSLLPGARGPALLPLSAGVRVIPVEIKEAGGGVPG
  SSPEDAAFQAPLAQESCCKFSSSQEAEEASGCPRKKDSSPMVICQLKGGAQMLCIDNCGARELKALHLLP
  QYDDQSSFPQSELPKPMTTLVGRLLPVPAKLNLITQVDNGALPSAVNGAAFPSGPALQGPPKIALAGYCD
  CFSSGDFCNSCSCNNLRHELERFKAIKACLDRNPEAFQPKMGKGRLGAAKLRHSKGCNCKRSGCLKNYCE
  CYEAKITCSSICKCIACKNYEESPERKMLMSTPHYMEPGDFESSHHLSPAKFSGPPRLRKNRQAFSCISW
  EVVEATCACLLAQGEEAEQERCSPSLAEQMVLEEFGRCLSQILHIEFKSKGLKIE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012069
• ORF Size: 1425 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012069.2, NP_001012069.2
• RefSeq Size: 2071 bp
• RefSeq ORF: 1428 bp
• Locus ID: 309142
• UniProt ID: Q5XHX9
• Cytogenetics: 1q42
• MW: 50.5 kDa
• Gene Summary: May have a role in spermatogenesis.[UniProtKB/Swiss-Prot Function]