March11 (NM_001101828) Rat Tagged ORF Clone

CAT#: RR206555

41344 (Myc-DDK-tagged ORF) - Rat membrane-associated ring finger (C3HC4) 11 (March11), (10 ug)

Product Images

Circular map for RR206555

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: March11
• Synonyms: MARCH-XI; RGD1559945
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206555 representing NM_001101828
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGACGAGGGCAGCAAACGGGGTAGCCGGGCGGACAGCCTCGAAGCTGAGCCACCTCTGCCCCCGC
  CGCCCCCACCCCCGCCTCCGGGAGAGTCCTCCCTGGTCCCCACCTCCCCACGCTACCGGCCGCCGCTACC
  CGCGCCTCTGGAGCGCATCGTAGGGTCGGGCGAGCCGCCGGTAGAACTGGCCCCGCGGCGCAAGGGTGAG
  CCGCTGCCACCGCTGCCGCCGTCTCGGTTGCCCGGCGACCAGGAGGTGTCAGCGGCGGGCGACTCCTGCG
  AAGGTCCTAGACGCCTTCCGGAGGTAAAGCTCCCGGAGGCTGCGGCGGGGAAAGGCAGCCCGGCGGAGCC
  CGAGGCCGGCGCGTGCGGGGAGGGCGAGCGGCGAGGCACCGGAGACCAGCCTGAGACACGCTCCGTATGT
  AGCAGCCGCAGCAGCAGTAGTGGCGGCAGTGGCGACCAGCGCTCGGGACATCAGCACCAGCACCATCAGC
  CCATTTGCAAGATCTGCTTCCAGGGCGCGGAGCAGGGTGAGTTGTTAAATCCTTGCCGATGTGATGGTTC
  TGTTCGGTACACACATCAGCTGTGTCTTCTAAAGTGGATCAGCGAGAGGGGCTCCTGGACCTGCGAACTG
  TGCTGTTATAGATACCACGTCACAGCCATTAAAATGAAGCAACCTTGCCAGTGGCAGAGCATTTCTATAA
  CACTGGTTGAGAAAGTTCAGATGATCGCTGTAATCCTAGGATCCCTGTTCTTAATAGCGAGTGTGACTTG
  GCTCCTCTGGTCAGCCTTCAGCCCCTATGCAGTGTGGCAGAGGAAGGACATCCTTTTCCAAATCTGCTAT
  GGAATGTATGGTTTTATGGATCTAGTGTGCATAGGCCTCATTGTCCATGAAGGAGCTGCAGTTTACAGAG
  TGTTTAAGCGATGGCGAGCTGTTAACTTGCACTGGGATGTATTAAATTATGACAAAGCCACAGACATTGA
  AGAAAGCAGCCGAGGAGAGTCATCCACAAGTAGGACTTTGTGGTTACCATTGTCAGCTCTGAGGAACAGG
  AACTTGGTCCACCCAACGCAACTAACCTCACCACGGTTTCAGTGTGGCTATGTGCTATTACATCTATTCA
  ATCGGATGAGGGCCCATGAAGATGTTTCGGAAGATAATGGCTCTGGTGAGGTAGTGATGAGAGTGACGTC
  TGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206555 representing NM_001101828
  Red=Cloning site Green=Tags(s)
  
  MSDEGSKRGSRADSLEAEPPLPPPPPPPPPGESSLVPTSPRYRPPLPAPLERIVGSGEPPVELAPRRKGE
  PLPPLPPSRLPGDQEVSAAGDSCEGPRRLPEVKLPEAAAGKGSPAEPEAGACGEGERRGTGDQPETRSVC
  SSRSSSSGGSGDQRSGHQHQHHQPICKICFQGAEQGELLNPCRCDGSVRYTHQLCLLKWISERGSWTCEL
  CCYRYHVTAIKMKQPCQWQSISITLVEKVQMIAVILGSLFLIASVTWLLWSAFSPYAVWQRKDILFQICY
  GMYGFMDLVCIGLIVHEGAAVYRVFKRWRAVNLHWDVLNYDKATDIEESSRGESSTSRTLWLPLSALRNR
  NLVHPTQLTSPRFQCGYVLLHLFNRMRAHEDVSEDNGSGEVVMRVTSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001101828
• ORF Size: 1194 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001101828.1, NP_001095298.1
• RefSeq Size: 1572 bp
• RefSeq ORF: 1197 bp
• Locus ID: 499558
• UniProt ID: A6P320
• Cytogenetics: 2q22
• MW: 43.9 kDa
• Gene Summary: E3 ubiquitin-protein ligase that mediates polyubiquitination of CD4. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May play a role in ubuquitin-dependent protein sorting in developmenting spermatids.[UniProtKB/Swiss-Prot Function]