Plb1 (NM_138898) Rat Tagged ORF Clone

CAT#: RR206357

Plb1 (Myc-DDK-tagged ORF) - Rat phospholipase B (Phlpb), (10 ug)

Product Images

Circular map for RR206357

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Plb1
• Synonyms: Phlpb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206357 representing NM_138898
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCATGGCCAGGCGTTTCCCTGGTGGGACTGCTGCTGCTGCTACTGCTGGGACAAGGGCCCTCCC
  AAATCCACGGCTCTTCTGGAGAAAACACATCGCAGCCCCAGCAAGTGTTTCGGACCCTGAAGAATTTTTC
  ATTCCCTTGCAAGCCAAAGAAGTTAGAACTGAGTGTGCTTTCTAAGTCAGTTCACTCTCTGAGACCCTCA
  GACATTAAACTCGTGGCAGCCATCGGCAACCTAGAAACTCCTCCAGCCCCTGGCTCAGGCGTGGTCAACA
  TGGAGAAACCTCAAAGCCTCGAGAGCGAACTACAGAATGTGTGCATAGGAATCATGACAGCCCTTTCAGA
  TATCATCAGACATTTCAACCCTTCTGTTCTGATGCCCACGTGTTCTCCTGGGAAGGGTACTGCAGGCCAC
  ACTACTATTGCCGAAGACTTGTGGATTCAGGCTAAAGAGCTGGTGAGGCATCTGAAAGACAACCCGGAAC
  TTGATTTTGAGAAGGACTGGAAACTCATCACTGTGCTCTTCAGTAACACAAGCCAGTGCCACCTGTGTTC
  CTCTGATCAGCAGAAAAGGCACTTGATGAAGCACATGGAGATGCTGTCAGGGGTGCTGGATTACCTGCAT
  CGTGAGGTCCCCAGAGCGTTTGTGAATTTGGTGGATCTCTCTGAGGTTTTGACCATGGCTCAGCAGCATC
  AAGAGACTGGTTTCAGCCCTGCACCAGAGATTTGCAAATGCTCAGAGGAAATAACGAAGTTATCCAAGGC
  TGTCATGCAGTGGTCCTATCAGGAAGCCTGGGAAGATCTCCTGGCCTCCAGCAAGTTCAATAAGCATGAG
  ACCTTCGCTGTGGTTTTCCAGTCTTTCTTCTCTGAGGTAGAACTACCCTTGGAGAGGCCCTCGCCCCAGG
  ATTCCACCACGCTCGCCCTCAGGATCTGGAATAGCATGATGGAACCAGTGGGTCGAAAGGATGGGACACT
  CAATGAAGCAGAGAGAAAAACAATGAAATGTCCCTCTCAGGAGAGCCCCTATCTGTTCACCTACAGAAAT
  AGCAACTACCAGGCCAGACAGCTGAAACCCATAGGAAAGTTTCAGATGAAAGAAGGAACAAAATTCACCT
  GTCCTGACAAAGACCCCTCGGACTCCATCCCCACAACAGTTCACAGGCTGAGGCCGGCTGACATCAAGGT
  CATCGGAGCCATGGGTGACTCGCTCACGGCAGGCAACGGGGCAGGGTCCAGCCCTGGGAATGTCTTGGAT
  GTCTTAACTCAATACCGAGGCCTGTCGTGGAGTGTGGGCGGAGATGAGACCATCGAGACCGTGACCACCC
  TAGCCAACATCCTCCGGGAATTCAACCCCTCCTTGAAGGGCTTCTCTGTTGGCACTGGGAAAGAAAACAC
  TCCCCGAGCATCCTTCAACCAGGCCGTAGCAGGAGCCAAATCTGATGGCTTAGCTGCCCAGGCCAAAAAG
  CTGGTGAGCCTGATGAAGGATGACAAGACAATAAACTTTCAGGAAGACTGGAAGATAATCACTGTGTTTA
  TAGGAGGCAACGACCTCTGTGGCTCCTGCAATAACCTGGCTCGCTTTTCTCCCCAAACCTTCACAGACAA
  CATCAAGACGGCCCTGGACATCCTCCATGCAGAGGTTCCCCGGGCCTTTGTGAACATGGTCTCGGTGATT
  GAGATCACCCCCTTGAGAGAACTGTTCAATGAACCTAAAGTCAGCTGCCCACGGATGATCCTCAGGAGCC
  TGTGTCCTTGTGTCTTGAACCTTGGTGAGAACTCAGCAGAACTTGCCCAACTTGTGGAAAGGAACAGGCA
  GTATCAGGAAGAAACTGGAAAACTGATTGAGAGTGGCCGATACGACACCAGGGATGACTTCACCGTGGTC
  CTCCAGCCCATGTTTGAAAATGTCGTCATGCCACGGACCCTGGAGGGCTTGCCCGACAGCTCTTTCTTTG
  CCCCTGACTGTTTCCACTTCAATGTCAAGACTCACGCTCGCTCAGCCATCGCCCTCTGGAAGAACATGCT
  AGAACCTGTGGGCCGCAAGACAAGACATCAGAATTTTGAAATCAAGGTCCCTATCATGTGTCCAAACCAG
  ACCTCACCGTTTCTGAGCACCACCAAGAACAGCAACCTGGGACATGGAACTTCGATGTCTTGTGAGGAGA
  AAGCCCCCTCTGCCTCACCACCAACCTCAGTGCATACCCTGAGACCTGCAGACATCCAAGTTGTGGCAGC
  TCTGGGAGACTCTGTGACTGCTGGCAATGGAATCAGCTCCCAAGAAGGTGATCTCGCTGATGTTACCACA
  CAGTATCGAGGACTGTCCTACAGTGCTGGTGGGGACAAGTTCCTGGAGAATGTGACCACCTTGCCCAACA
  TCCTCCGGGAATTTAATGGAAATCTCACAGGCTACTCAGTCGGAACCGGTGACGTCAACTCTGCAAGCGC
  GTTCCTTAACCAGGCTGTCCCTGGGGCAAAGGCTGAGAACCTTGCAAGTCAAGTCCAGACTCTGATTCAG
  AAGATGAAGAATGACACCAGAGTGAACTTTCACCAAGACTGGAAGGTCATCACTGTGATGATTGGGGCCA
  GCGACTTGTGTGACTTCTGCAAGGATTCGAATCGTTACTCTGCAGCCAATTTTTCTGACCATCTCCGCAA
  TGCCTTGGACATCCTGCATAAGGAGGTACCCAGAGCCCTGGTCAACCTTGTGGACTTCATGAACCCCAGT
  ATCATTCGGCAAGTGTTCCTGAAGAACCCAGACAAGTGCCCTGTGAATCAGACCAGTGTCCTGTGCAACT
  GTGTTCTGACCCCAGGGGAGGATTCCCATGAGCTGGCAAGGTTGGAGGCCTTCACCAAATCCTACCAGAG
  TAGCATGCTTCAACTGGTTGAGTCAGGCCGCTACGACACCCGGGAGGATTTCTCTGTGGTACTGCAGCCC
  TTTCTCTTCAACATCAGGCTCCCCATCCTAGAGAATGGGAATCCAGATACATCCTTCTTTGCCCCAGACT
  GCATCCTCCTAAGCCAGAAGTTCCACACTCAGCTCGCGAGAGCCCTTTGGGCCAATATGCTTGAACCCCT
  GGGAAAGAAAATGGATACTTTGGACCCGAAAGAACTCATAGCTTTGGCCTGCCCCACCAAGGACAAGCCC
  TTCCTGAGAACCTTCCGGAACAGTAACTACACGTACCCTATCAAGCCAGCCATTGAGAATTGGGGCAGTG
  ACTTCCTGTGCACAGAGCAGAGTCCTTCCAGCAAGGTACCCACCTCAGTTCATGAGCTCCGACCATCAGA
  CATCAAGGTGGTGGCAGCAATGGGTGACTTTCTGACGACAGCCACCGGAGCTCGACCAAGTGAGTCCAGC
  AGTCTAGACACCCCCTGGAGGGGGCTGTCTTGGAGCATTGGAGGAGATGGAACGTTGGAGACCCATACCA
  CACTGCCCAACATCCTGAAGAAGTTCAACCCTTCCATCCTTGGATTCTCCACCGGTACCCTGGAGAACAC
  GGCAGGATTAAATGTGGCAGAAGAAGGCGCCAGAGCTCAGGACATGCCGGCCCAGGCTCAGGCCCTGGTG
  AAGAAGATGAAAAGCACCCCTACAATCAACATACAGGAAGACTGGAAGCTGATTACACTCCTCATCGGGA
  ACAACGACCTGTGTCTTTACTGTGAGGATCCGGAGAACTACTCAACCAGGGAGTATGTCAAGTACATCCA
  GCATGCCTTGGACATCTTCTATGAGGAGCTTCCCAGGGTTTTCATCAACGTGGTGGAAGTCATGGAGCTG
  TCCGGTCTGCTCCACGACCAGGGCGGGAAATGTGCCATGCCGTTGGCTGTCCAGAAAAACTGCAGTTGCC
  TTAAACGCTCTCAAAACCTCATGGCAATGCAGGAGCTGAAGAAAGTCAACGGGAACCTCCAGAGTGCCCT
  CTCGGAGCTCTCCTACTGGCATCGGTACATGCAGCGTGAGGACTTTGCAGTCACTGTCCAGCCTTTCTTC
  CGGAATACCTTTGTCCCACTGGATGAGCGTGGGGGCCTCGACCTCACTTTCTTCTCTGAAGACTGTTTCC
  ACTTCTCAGTCCGTGGGCATGCTGAGATGGCCATTGCCCTCTGGAACAACATGCTGGAACCAGTGGGCAA
  GAAGACAACCTCCAATAACTTCACATACAACCGGACCAAACTCAAGTGTCCCTCGCCTGAAAACCCTTTC
  CTCTACACTGTCCGGAACAGTCAGATTCTTCTAGACAAGGCTAAAGAAAACTCCAATACACTCTACTGGG
  CAGTGCCAGTGGCTGCAGTAGGTGGCCTGGTAGTTGGCATCCTTGGAATGATGTTGTGGAGAACTGTGAG
  ACTCGTCCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206357 representing NM_138898
  Red=Cloning site Green=Tags(s)
  
  MESWPGVSLVGLLLLLLLGQGPSQIHGSSGENTSQPQQVFRTLKNFSFPCKPKKLELSVLSKSVHSLRPS
  DIKLVAAIGNLETPPAPGSGVVNMEKPQSLESELQNVCIGIMTALSDIIRHFNPSVLMPTCSPGKGTAGH
  TTIAEDLWIQAKELVRHLKDNPELDFEKDWKLITVLFSNTSQCHLCSSDQQKRHLMKHMEMLSGVLDYLH
  REVPRAFVNLVDLSEVLTMAQQHQETGFSPAPEICKCSEEITKLSKAVMQWSYQEAWEDLLASSKFNKHE
  TFAVVFQSFFSEVELPLERPSPQDSTTLALRIWNSMMEPVGRKDGTLNEAERKTMKCPSQESPYLFTYRN
  SNYQARQLKPIGKFQMKEGTKFTCPDKDPSDSIPTTVHRLRPADIKVIGAMGDSLTAGNGAGSSPGNVLD
  VLTQYRGLSWSVGGDETIETVTTLANILREFNPSLKGFSVGTGKENTPRASFNQAVAGAKSDGLAAQAKK
  LVSLMKDDKTINFQEDWKIITVFIGGNDLCGSCNNLARFSPQTFTDNIKTALDILHAEVPRAFVNMVSVI
  EITPLRELFNEPKVSCPRMILRSLCPCVLNLGENSAELAQLVERNRQYQEETGKLIESGRYDTRDDFTVV
  LQPMFENVVMPRTLEGLPDSSFFAPDCFHFNVKTHARSAIALWKNMLEPVGRKTRHQNFEIKVPIMCPNQ
  TSPFLSTTKNSNLGHGTSMSCEEKAPSASPPTSVHTLRPADIQVVAALGDSVTAGNGISSQEGDLADVTT
  QYRGLSYSAGGDKFLENVTTLPNILREFNGNLTGYSVGTGDVNSASAFLNQAVPGAKAENLASQVQTLIQ
  KMKNDTRVNFHQDWKVITVMIGASDLCDFCKDSNRYSAANFSDHLRNALDILHKEVPRALVNLVDFMNPS
  IIRQVFLKNPDKCPVNQTSVLCNCVLTPGEDSHELARLEAFTKSYQSSMLQLVESGRYDTREDFSVVLQP
  FLFNIRLPILENGNPDTSFFAPDCILLSQKFHTQLARALWANMLEPLGKKMDTLDPKELIALACPTKDKP
  FLRTFRNSNYTYPIKPAIENWGSDFLCTEQSPSSKVPTSVHELRPSDIKVVAAMGDFLTTATGARPSESS
  SLDTPWRGLSWSIGGDGTLETHTTLPNILKKFNPSILGFSTGTLENTAGLNVAEEGARAQDMPAQAQALV
  KKMKSTPTINIQEDWKLITLLIGNNDLCLYCEDPENYSTREYVKYIQHALDIFYEELPRVFINVVEVMEL
  SGLLHDQGGKCAMPLAVQKNCSCLKRSQNLMAMQELKKVNGNLQSALSELSYWHRYMQREDFAVTVQPFF
  RNTFVPLDERGGLDLTFFSEDCFHFSVRGHAEMAIALWNNMLEPVGKKTTSNNFTYNRTKLKCPSPENPF
  LYTVRNSQILLDKAKENSNTLYWAVPVAAVGGLVVGILGMMLWRTVRLVQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138898
• ORF Size: 4350 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138898.1, NP_620253.1
• RefSeq Size: 4590 bp
• RefSeq ORF: 4353 bp
• Locus ID: 192259
• UniProt ID: O54728
• Cytogenetics: 6q14
• MW: 161.1 kDa
• Gene Summary: exhibits broad lipolytic activities (PLA2, lysophospholipase, and lipase activities); may mediate terminal digestion, or membrane digestion, of dietary lipids and biliary phospholipids [RGD, Feb 2006]