Runx3 (NM_130425) Rat Tagged ORF Clone

CAT#: RR206327

Runx3 (Myc-DDK-tagged ORF) - Rat runt-related transcription factor 3 (Runx3), (10 ug)

Product Images

Circular map for RR206327

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Runx3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206327 representing NM_130425
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTATTCCCGTAGACCCAAGCACCAGCCGCCGCTTCACTCCCCCCTCCACGGCCTTCCCCTGCGGCG
  GCGGCGGCGGCGGCAAGATGGGCGAGAACAGCGGCGCGCTGAGCGCGCAGGCGGCCGCGGGCCCCGGCGG
  CCGCACCCGGCCCGAAGTGCGCTCGATGGTGGACGTGCTGGCCGACCACGCGGGAGAGCTCGTGCGCACC
  GACAGCCCCAACTTCCTCTGCTCCGTGCTGCCCTCGCACTGGCGCTGCAACAAGACGCTGCCGGTCGCCT
  TCAAGGTGGTGGCACTGGGGGATGTTCCTGATGGAACGGTGGTGACCGTGATGGCGGGCAATGACGAGAA
  TTACTCCGCCGAGCTGCGCAACGCTTCCGCTGTCATGAAGAACCAAGTGGCCCGGTTCAACGACCTCCGC
  TTCGTGGGCCGCAGCGGGCGAGGGAAGAGCTTCACGCTCACAATCACCGTGTTCACCAACCCTACCCAAG
  TGGCCACCTACCACCGAGCCATCAAGGTTACTGTGGATGGACCCCGGGAACCCCGACGGCACCGGCAGAA
  GATAGAGGACCAGACCAAAGCCTTCCCCGACCGCTTCGGAGACCTGCGCATGCGTGTAACACCAAGCACA
  CCCAGCCCCCGTGGCTCTCTCAGCACCACGAGCCACTTCAGCAGCCAGGCCCAGACCCCAATCCAAGGCT
  CCTCAGACCTGAACCCCTTCTCGGACCCCCGCCAGTTTGATCGCTCCTTCCCTACGCTGCAAAGCCTCAC
  AGAGAGCCGCTTCCCGGAGGGAAGGATGCACTACCCGGGAGCCATGTCTGCCGCCTTCCCCTACAGTGCC
  ACACCATCGGGAACCAGCCTGGGCGGCCTGAGCGTGGCCGGCATGCCGGCCAGCAGCCGCTTCCACCACA
  CCTACCTCCCTCCGCCCTACCCGGGGGCCCCGCAGAGTCAGAGCGGGCCCTTTCAGGCCAACCCTGCGCC
  CTACCACCTCTTCTATGGCACCTCCTCCGGCTCCTACCAGTTCTCCATGGCAGCTGCCGGAGGTGGCGAG
  CGCTCGCCCACCCGCATGCTGACCTCCTGCCCCACCGGCGCTTCGGTGTCAGCAGGCAATCTCATGAACC
  CCAGCCTGGGCCAGGCTGATGGCGTGGAAGCCGACGGCAGTCACAGCAACTCGCCCACGGCCCTGAGCAC
  ACCGGGCCGCATGGACGAGGCCGTGTGGCGGCCCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206327 representing NM_130425
  Red=Cloning site Green=Tags(s)
  
  MRIPVDPSTSRRFTPPSTAFPCGGGGGGKMGENSGALSAQAAAGPGGRTRPEVRSMVDVLADHAGELVRT
  DSPNFLCSVLPSHWRCNKTLPVAFKVVALGDVPDGTVVTVMAGNDENYSAELRNASAVMKNQVARFNDLR
  FVGRSGRGKSFTLTITVFTNPTQVATYHRAIKVTVDGPREPRRHRQKIEDQTKAFPDRFGDLRMRVTPST
  PSPRGSLSTTSHFSSQAQTPIQGSSDLNPFSDPRQFDRSFPTLQSLTESRFPEGRMHYPGAMSAAFPYSA
  TPSGTSLGGLSVAGMPASSRFHHTYLPPPYPGAPQSQSGPFQANPAPYHLFYGTSSGSYQFSMAAAGGGE
  RSPTRMLTSCPTGASVSAGNLMNPSLGQADGVEADGSHSNSPTALSTPGRMDEAVWRPY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130425
• ORF Size: 1227 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130425.1, NP_569109.1
• RefSeq Size: 1230 bp
• RefSeq ORF: 1230 bp
• Locus ID: 156726
• Cytogenetics: 5q36
• MW: 43.5 kDa
• Gene Summary: mouse homolog is a transcription factor required for establishing epigenetic silencing in cytotoxic lineage thymocytes [RGD, Feb 2006]