Psma3 (NM_017280) Rat Tagged ORF Clone

CAT#: RR206128

Psma3 (Myc-DDK-tagged ORF) - Rat proteasome (prosome, macropain) subunit, alpha type 3 (Psma3), (10 ug)

Product Images

Circular map for RR206128

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Psma3
• Synonyms: Psma3l
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206128 representing NM_017280
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCCATTGGCACTGGGTATGACCTGTCAGCCTCTACATTCTCTCCTGATGGAAGAGTTTTCCAAG
  TTGAATATGCTATGAAGGCTGTGGAAAACAGTAGTACAGCTATTGGGATCAGATGTAAAGATGGTGTTGT
  GTTTGGGGTAGAAAAACTAGTCCTTTCTAAACTTTATGAAGAAGGCTCCAATAAACGTCTTTTCAATGTT
  GATCGACATGTTGGAATGGCAGTGGCAGGTTTGTTGGCAGATGCTCGTTCCTTGGCAGACATAGCAAGAG
  AAGAAGCGTCCAACTTTAGGTCTAACTTTGGCTATAACATTCCTCTAAAACATCTTGCAGACAGAGTGGC
  CATGTATGTACACGCGTATACACTCTACAGTGCTGTTAGACCTTTTGGCTGCAGTTTCATGTTGGGGTCT
  TACAGTGTGAATGATGGTGCACAGCTCTATATGATCGATCCATCAGGTGTTTCATATGGTTACTGGGGCT
  GTGCCATTGGCAAAGCCAGGCAAGCTGCAAAGACAGAAATAGAAAAGCTTCAGATGAAGGAAATGACCTG
  CCGTGATGTAGTTAAAGAAGTTGCAAAAATAATTTACATAGTACATGATGAAGTTAAGGATAAAGCTTTT
  GAACTAGAGCTCAGCTGGGTTGGTGAATTAACTAAAGGAAGACATGAAATTGTTCCCAAAGACGTAAGAG
  AGGAAGCAGAGAAATATGCCAAGGAATCTTTGAAGGAAGAAGATGAATCAGATGACGACAATATG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206128 representing NM_017280
  Red=Cloning site Green=Tags(s)
  
  MSSIGTGYDLSASTFSPDGRVFQVEYAMKAVENSSTAIGIRCKDGVVFGVEKLVLSKLYEEGSNKRLFNV
  DRHVGMAVAGLLADARSLADIAREEASNFRSNFGYNIPLKHLADRVAMYVHAYTLYSAVRPFGCSFMLGS
  YSVNDGAQLYMIDPSGVSYGYWGCAIGKARQAAKTEIEKLQMKEMTCRDVVKEVAKIIYIVHDEVKDKAF
  ELELSWVGELTKGRHEIVPKDVREEAEKYAKESLKEEDESDDDNM
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017280
• ORF Size: 765 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017280.2, NP_058976.1
• RefSeq Size: 1993 bp
• RefSeq ORF: 768 bp
• Locus ID: 29670
• UniProt ID: P18422
• Cytogenetics: 6q24
• MW: 28.4 kDa
• Gene Summary: component of the proteasome multicatalytic proteinase complex [RGD, Feb 2006]