Hsd17b13 (NM_001009684) Rat Tagged ORF Clone

CAT#: RR206057

Hsd17b13 (Myc-DDK-tagged ORF) - Rat hydroxysteroid (17-beta) dehydrogenase 13 (Hsd17b13), (10 ug)

Product Images

Circular map for RR206057

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hsd17b13
• Synonyms: MGC108860
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206057 representing NM_001009684
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCTCATCCTGGAGCTTCTCTTGCTGGTGGGCATCATCATCTACTCCTACCTGGAGTCACTGGTAA
  AGTTCTTCATTCCCCAGAGAAGAAAGTCTGTGGCCGGACAGACTGTTCTCATCACAGGAGCTGGACACGG
  AATAGGCAGGCTGACGGCTTATGAATTTGCAAAGCAGAAAAGCAGATTGGTCCTGTGGGATATTAGTAAG
  CACGGTGTTGAGGAAACCGCAGCCAAATGCAGGAAACTGGGGGCTGTCGTGCACGTGTTTGTGGTGGACT
  GCAGCAACCGGGCAGAGATTTACAAGTCTGTGGATCAGGTAAAGAAAGAAGTTGGTGACATAGAGATTGT
  GGTAAACAACGCTGGGGCAATTTATCCAGCCGACCTTCTTAGCACCAAGGATGAGGAGATCACCAAGACC
  TTCGAGGTCAACATCCTAGGACATTTTTGGATCATAAAAGCACTCCTTCCATCGATGCTGAGAAGAAACT
  CTGGCCACATTGTCACAGTGGCTTCAGTGTGTGGCCACAGAGTGATTCCTTATCTCATCCCATATTGCTC
  CAGCAAGTTCGCTGCTGTGGGCTTCCACAGAGCACTGACTGCAGAACTGGACACCTTGGGGAAAACCGGT
  ATCAAAACCTCCTGTCTCTGCCCTGTGTTCGTGAATACTGGCTTCACCAAAAACCCAAGTACCAGGTTAT
  GGCCTGTGTTAGAGCCGGATGAAGTTGCAAGGAGTCTGATCGATGGGATACTTACCAACAAGAAAATGAT
  CTTCGTTCCGTCCTATATCAATATCTCTCTAATAGTGGAAATGTTTTTTCCTGAACGTGTCTTAAAAGCT
  ATAAACCGTATACAGAATATTCAGTTTGAAGCGATTGTGGGCCACAGAACCAAGAGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206057 representing NM_001009684
  Red=Cloning site Green=Tags(s)
  
  MNLILELLLLVGIIIYSYLESLVKFFIPQRRKSVAGQTVLITGAGHGIGRLTAYEFAKQKSRLVLWDISK
  HGVEETAAKCRKLGAVVHVFVVDCSNRAEIYKSVDQVKKEVGDIEIVVNNAGAIYPADLLSTKDEEITKT
  FEVNILGHFWIIKALLPSMLRRNSGHIVTVASVCGHRVIPYLIPYCSSKFAAVGFHRALTAELDTLGKTG
  IKTSCLCPVFVNTGFTKNPSTRLWPVLEPDEVARSLIDGILTNKKMIFVPSYINISLIVEMFFPERVLKA
  INRIQNIQFEAIVGHRTKRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009684
• ORF Size: 900 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009684.1, NP_001009684.1
• RefSeq Size: 1323 bp
• RefSeq ORF: 903 bp
• Locus ID: 305150
• UniProt ID: Q5M875
• Cytogenetics: 14p22
• MW: 33.5 kDa