Ncf4 (NM_001127304) Rat Tagged ORF Clone

CAT#: RR205963

Ncf4 (Myc-DDK-tagged ORF) - Rat neutrophil cytosolic factor 4 (Ncf4), (10 ug)

Product Images

Circular map for RR205963

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ncf4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205963 representing NM_001127304
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGGCCCAGCAGCTGCGATCAGAGAGCGATTTTGAGCAGCTTCCAGATGATGTGGCCATCTCAG
  CCAACATCGCTGACATCGAGGAGAAGAGGGGCTTCACCAGCCACTTTGTGTTTGTCATCGAGGTAAAGAC
  AAAAGGAGGATCCAAGTATCTCATCTACCGCCGATATCGCCAGTTCTACGCCCTGCAGAGCAAGCTGGAG
  GAGCGGTTTGGACCAGAGAGCAAGAACAGCCCATTCACCTGCAGCCTGCCCACACTGCCAGCCAAAGTCT
  ACATGGGTGTCAAACAAGAGATTGCTGAGACTCGGATCCCGGCCCTCAATGCCTACATGAAGAACCTCCT
  GAGCCTGCCGGTCTGCGTGCTGATGGACCCCGACGTCAGAATATTCTTCTATCAGTCTGCTTACGACGCA
  GAGCAGGTGCCTCAGGCACTTCGTAGGCTCCGACCGCGCACGCGCAAAATCAAGGGCGTGACTCCACAAG
  GGCCCAGCATGGACCGCATGGAAGCTCCAAGAGCAGAGGCCTTGTTTGACTTCACTGGGAACAGCAAACT
  GGAGCTAAGTTTCAAAGCTGGAGATGTGATCTTCCTTCTCAGTAAGATCAACAAAGACTGGTTGGAGGGC
  ACAACCCAGGGAGCCACAGGCATCTTCCCAGGGTCTTTCGTGAAGATCCTCAAGGACTTCCCCGAAGACG
  AGGACACTACCAACTGGCTGCGATGCTACTTCTACGAAGACACAGGCAAAACCATCAAGGACATTGCAGT
  GGAGGAGGACCTCAGCAGCACCCCCCTGTTCAAAGACCTGCTAGCACTCATGAGGCGTGAATTCCAGAGA
  GAGGACATTGCCCTTAATTACCAAGATGCTGAGGGGGACCTGGTTCGGCTGCTGTCAGATGAGGATGTGG
  GACTCATGGTGAAGCAGGCCCAAGGCCTCCCCTCCCAGAAGCGCCTCTTCCCCTGGAAGCTGCACGTCAC
  CCAGAAGGACAATTACGGTGTCTACAACACCGTACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205963 representing NM_001127304
  Red=Cloning site Green=Tags(s)
  
  MALAQQLRSESDFEQLPDDVAISANIADIEEKRGFTSHFVFVIEVKTKGGSKYLIYRRYRQFYALQSKLE
  ERFGPESKNSPFTCSLPTLPAKVYMGVKQEIAETRIPALNAYMKNLLSLPVCVLMDPDVRIFFYQSAYDA
  EQVPQALRRLRPRTRKIKGVTPQGPSMDRMEAPRAEALFDFTGNSKLELSFKAGDVIFLLSKINKDWLEG
  TTQGATGIFPGSFVKILKDFPEDEDTTNWLRCYFYEDTGKTIKDIAVEEDLSSTPLFKDLLALMRREFQR
  EDIALNYQDAEGDLVRLLSDEDVGLMVKQAQGLPSQKRLFPWKLHVTQKDNYGVYNTVP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001127304
• ORF Size: 1017 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127304.1, NP_001120776.1
• RefSeq Size: 1496 bp
• RefSeq ORF: 1020 bp
• Locus ID: 500904
• Cytogenetics: 7q34
• MW: 38.7 kDa