Cd55 (NM_022269) Rat Tagged ORF Clone

CAT#: RR205550

Cd55 (Myc-DDK-tagged ORF) - Rat Cd55 molecule (Cd55), (10 ug)

Product Images

Circular map for RR205550

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cd55
• Synonyms: Daf; Daf1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205550 representing NM_022269
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCCGCACGCGGGCGCCGGGGACTCGGGCACCGCCGCCGCCGCCGCTGCTGCTGTCGCTGTCGCTGC
  TGCTGCTGCTGCTGTCCCCGACTGTAAGCGGAGACTGCGGCCCACCTCCAGACATTCCTAATGCCACGCC
  AAACTCGGGTAAACACACCAAGTTTGCCCACCAAAGCCAAGTGACATATTCGTGTAATAAGGGCTTCAAA
  CAAATTCCAGACAAGCCAAACACAGTGGTCTGCCTTGAAAATGACCAATGGTCTAACTACGAAACTTTCT
  GTAATAAAACCTGTAGTGCTCCACTGAGACTGAATTATGCATCCCTCAAAAAGGAGTATCGTAACATGAA
  TTTTTTCCCAATTGGCACCACTGTGGAATTTGAGTGTCGGCCAGGATTTCAAAAAGTAACTTCACGCACA
  GGAAAATCAACTTGCCTTGAGGAATTAGTATGGTCTCCAGTTGTTGAATTTTGTAAAAAAAGAGCATGCC
  CTAATCCTAGAGAACTGAAAAATGGTCACATCAACATACCAACTGACATATTATTCGGATCAGAAATAAT
  CTTCTCATGCGACAAAGGGTACAAGCTGGTCGGTGTAACTTCTACATTCTGTTCTATTACGGGGAATGCT
  GTGGACTGGGATGACGTATTTCCAGTGTGCACAACAATTTTTTGTCCAGACCCACCAAAAATCAATAATG
  GAAGAATTCGAGAGGAAAGCGACTCCTACGCATATAGACAGTCGGTGACCTATTCCTGTGACCAAGGCTT
  CATTCTGGTTGGAAATTCTTCCGTTCATTGTACTCTGAACGGTGAAGTAGGAGAATGGAGTAGTGCACCA
  CCCAAGTGCATAGAGAGACACAAGGTCCCAACTAAGACCCCACCAGAAGTTGATATTCCAAGTACAATGC
  CTCATGAATCCACCTTAATTAATTTTCCAAGTACAAGAGCCCCACTGTCTCATAAACCCACTACAGTTAA
  AGTTCCAGGTACAAGAGTCAAACCAACGTCTCACAAGCCCACTGAAGTTAAAGTTCCAGCAACACAGCAT
  GTACCTGTTTCCAAGACAACGGTACGTCATCCAACGAGAACATCTAAAGACAGAGGAGAGTCTAATTCAG
  GTGGTGACCATTTTATATATGGACATACATGTTTAATAACCATGACAATTTTGCATGTGATGCTCTTACT
  CACTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205550 representing NM_022269
  Red=Cloning site Green=Tags(s)
  
  MIRTRAPGTRAPPPPPLLLSLSLLLLLLSPTVSGDCGPPPDIPNATPNSGKHTKFAHQSQVTYSCNKGFK
  QIPDKPNTVVCLENDQWSNYETFCNKTCSAPLRLNYASLKKEYRNMNFFPIGTTVEFECRPGFQKVTSRT
  GKSTCLEELVWSPVVEFCKKRACPNPRELKNGHINIPTDILFGSEIIFSCDKGYKLVGVTSTFCSITGNA
  VDWDDVFPVCTTIFCPDPPKINNGRIREESDSYAYRQSVTYSCDQGFILVGNSSVHCTLNGEVGEWSSAP
  PKCIERHKVPTKTPPEVDIPSTMPHESTLINFPSTRAPLSHKPTTVKVPGTRVKPTSHKPTEVKVPATQH
  VPVSKTTVRHPTRTSKDRGESNSGGDHFIYGHTCLITMTILHVMLLLTG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022269
• ORF Size: 1197 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022269.2, NP_071605.2
• RefSeq Size: 1836 bp
• RefSeq ORF: 1200 bp
• Locus ID: 64036
• Cytogenetics: 13q13
• MW: 44 kDa
• Gene Summary: accelerates the decay of the classical and alternative pathway C3 convertases, C4b2a and C3bBb [RGD, Feb 2006]