Robo1 (NM_022188) Rat Tagged ORF Clone

CAT#: RR205390

Robo1 (Myc-DDK-tagged ORF) - Rat roundabout homolog 1 (Drosophila) (Robo1), (10 ug)

Product Images

Circular map for RR205390

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Robo1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205390 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATGGAAACATCTTCCTCTTCTGGTCATGATATCTCTCCTCACTCTATCAAAAAAGCATCTCCTTC
  TGGCCCAGCTTATTCCAGACCCTGAGGACCTAGAGAGGGGGAACGACAACGGGACGCCAGCCCCCACCTC
  TGATAATGATGACAATTCGCTGGGCTACACAGGCTCCCGTCTCCGTCAGGAAGATTTCCCACCGCGCATT
  GTTGAGCACCCTTCAGACCTGATCGTCTCTAAAGGGGAACCCGCCACCCTCAACTGTAAAGCTGAAGGCC
  GCCCTACCCCGACTATAGAATGGTACAAGGGCGGCGAAAGAGTGGAAACGGACAAAGACGACCCTCGCTC
  ACACCGAATGCTGCTGCCAAGTGGATCTTTATTTTTCTTACGCATAGTGCATGGACGGAAGAGTAGGCCT
  GACGAAGGGGTCTACATCTGTGTAGCAAGGAATTACCTCGGAGAGGCTGTGAGCCACAATGCATCGCTGG
  AGGTAGCTATACTACGGGATGACTTCAGACAAAACCCTTCAGATGTTATGGTGGCGGTGGGCGAGCCTGC
  AGTAATGGAATGCCAGCCTCCTCGGGGCCACCCAGAGCCCACCATCTCCTGGAAAAAGGATGGCTCACCC
  CTGGATGATAAAGACGAGCGAATCACTATCCGAGGCGGGAAGCTCATGATCACGTACACCCGTAAAAGCG
  ATGCTGGCAAGTATGTTTGTGTTGGTACCAACATGGTAGGGGAGCGGGAGAGCAAAGTCGCGGACGTGAC
  TGTCTTAGAGAGACCGTCCTTTGTAAAGAGACCCAGTAATTTGGCAGTGACTGTGGATGACAGTGCTGAG
  TTTAAGTGTGAGGCCCGAGGAGACCCCGTGCCTACGTTCGGGTGGAGGAAAGATGACGGAGAGCTGCCCA
  AATCCAGATATGAGATCCGAGATGATCATACTTTGAAAATTCGGAAGGTGACAGCTGGCGACATGGGATC
  ATACACTTGTGTTGCAGAAAATATGGTTGGGAAAGCCGAAGCATCAGCCACTTTGACAGTTCAAGAGCCA
  CCACATTTTGTCGTGAAGCCTCGAGACCAGGTGGTTGCCTTGGGGCGAACTGTGACTTTTCAGTGTGAAG
  CAACTGGAAATCCTCAACCAGCTATCTTTTGGCGGAGAGAGGGGAGTCAGAATCTGCTTTTCTCATACCA
  GCCTCCACAGTCCTCTAGCCGGTTTTCAGTTTCCCAGACGGGGGACCTCACTGTTACGAACGTCCAGAGG
  TCTGATGTCGGTTACTATATCTGTCAGACTTTGAACGTGGCTGGAAGCATCATCACAAAGGCATATTTGG
  AAGTTACTGATGTGATTGCGGACCGGCCTCCCCCAGTCATCCGACAAGGTCCTGTGAATCAGACTGTAGC
  AGTGGATGGTACTTTAACTCTCAGCTGTGTGGCCACAGGCAGTCCCGTGCCCACAATTCTGTGGAGGAAG
  GATGGAGTCCTTGTTTCAACCCAAGATTCTCGGATCAAACAGCTGGAGAGCGGGGTCCTGCAGATCCGCT
  ACGCTAAGCTGGGTGACACCGGTCGGTACACCTGCACTGCTTCGACCCCCAGTGGTGAGGCCACGTGGAG
  CGCTTACATCGAAGTCCAGGAATTTGGAGTTCCAGTTCAACCTCCAAGACCCACCGACCCCAATTTAATC
  CCCAGTGCGCCTTCAAAGCCTGAAGTCACAGATGTGAGCAAAAACACAGTCACTTTATTGTGGCAGCCTA
  ACCTGAACTCAGGAGCAACCCCAACGTCTTATATTATAGAAGCCTTCAGCCATGCATCTGGTAGCAGCTG
  GCAGACCGTGGCAGAAAATGTGAAAACAGAAACATTCGCCATCAAAGGGCTCAAACCTAATGCGATCTAC
  CTTTTCCTGGTGAGGGCAGCAAATGCCTATGGGATTAGCGATCCAAGCCAAATATCCGATCCAGTGAAAA
  CACAAGATGTCCCACCAACCACCCAAGGGGTGGACCACAAGCAGGTCCAGAGAGAACTGGGCAATGTTGT
  CTTGCATCTCCACAACCCTACCATCCTTTCTTCCTCTTCTGTCGAAGTGCACTGGACGGTGGACCAACAA
  TCTCAATATATTCAAGGTTATAAAATTCTCTACCGACCATCTGGAGCCAGCCATGGCGAATCGGAGTGGT
  TAGTTTTTGAAGTGAGGACCCCAACCAAAAATAGTGTGGTGATCCCCGATCTGAGAAAAGGCGTCAACTA
  CGAGATTAAGGCCCGCCCATTTTTTAATGAGTTCCAAGGAGCAGATAGTGAAATCAAATTTGCCAAAACC
  TTAGAAGAACGACCAAGTGCCCCTCCCCGAAGTGTCACTGTATCCAAGAATGATGGAAATGGGACAGCAA
  TTCTGGTCACTTGGCAACCGCCTCCTGAAGACACACAGAATGGAATGGTCCAAGAATATAAGGTTTGGTG
  TCTCGGGAATGAGACTAGGTATCACATAAACAAGACAGTAGATGGCTCTACCTTTTCGGTGGTCATTCCC
  TTTCTTGTTCCTGGAATCCGCTACAGTGTAGAAGTGGCTGCAAGCACTGGGGCTGGCCCTGGGGTGAAGA
  GTGAGCCCCAGTTCATCCAGTTGGATTCCCATGGAAACCCCGTGTCTCCCGAGGACCAAGTGAGCCTGGC
  TCAGCAGATCTCCGACGTGGTGAAGCAGCCAGCCTTCATTGCGGGCATCGGGGCCGCCTGTTGGATCATT
  CTCATGGTCTTCAGTATCTGGCTTTACCGACACCGCAAGAAGAGAAACGGGCTCAGCAGCACGTACGCGG
  GGATCCGGAAAGTCCCGTCCTTTACCTTCACACCAACAGTAACTTATCAAAGAGGAGGCGAAGCCGTCAG
  CAGTGGAGGGAGGCCGGGACTCCTCAACATCAGTGAACCTGCCACACAGCCATGGCTGGCGGACACGTGG
  CCCAACACAGGCAACAGTCACAATGACTGCTCCATCAACTGCTGCACAGCCAGCAACGGCAACAGCGACA
  GCAACCTGACCACCTACAGTCGCCCAGCTGATTGTATAGCAAATTATAACAACCAACTGGATAACAAACA
  AACAAATCTGATGCTCCCTGAGTCAACTGTTTATGGTGATGTGGACCTTAGTAACAAAATCAATGAGATG
  AAAACCTTCAATAGCCCAAATCTGAAGGATGGGCGTTTTGTCAATCCATCAGGGCAGCCCACTCCCTACG
  CCACCACGCAGCTCATCCAGGCAAATCTCATCAACAATATGAATAACGGCGGCGGGGACTCCAGCGAGAA
  ACACTGGAAGCCTCCGGGGCAGCAGAAACAGGAAGTGGCACCAATTCAGTATAACATCATGGAGCAAAAC
  AAGTTGAACAAAGATTATCGGGCAAATGACACAATCCTTCCAACCATCCCGTACAACCATTCNTATGACC
  AGAATACAGGAGGATCCTACAACAGCTCAGATCGGGGCAGTAGTACATCTGGGAGTCAAGGCCACAAGAA
  AGGGGCACGGACACCAAAGGCACCCAAGCAGGGTGGCATGAACTGGGCAGACTTGCTGCCCCCTCCCCCA
  GCGCATCCTCCCCCACACAGCAACAGCGAGGAATACAGCATGTCTGTAGATGAAAGCTATGATCAAGAAA
  TGCCGTGCCCTGTTCCGCCTGCACGGATGTATTTGCAACAGGATGAATTAGAAGAAGAGGAAGCTGAAAG
  AGGCCCCACGCCCCCTGTCCGGGGGGCAGCTTCGTCCCCAGCTGCTGTGTCCTACAGCCATCAGTCCACG
  GCCACTCTCACCCCGTCTCCCCAGGAAGAGCTCCAGCCTATGTTACAGGATTGTCCGGAGGACCTCGGCC
  ACATGCCCCACCCACCAGACAGGAGGCGGCAGCCGGTGAGTCCTCCTCCACCACCACGGCCAATTTCCCC
  ACCACATACTTACGGCTACATTTCAGGACCCCTGGTCTCAGACATGGATACGGATGCCCCAGAAGAGGAG
  GAAGACGAAGCTGACATGGAAGTTGCTAAGATGCAGACCCGAAGGCTGTTGTTACGTGGGCTTGAACAGA
  CCCCAGCATCTAGTGTTGGGGACCTCGAGAGCTCGGTCACTGGCTCCATGATCAATGGCTGGGGCTCAGC
  CTCAGAAGAGGACAACATTTCCAGTGGGCGCTCCAGTGTCAGTTCATCGGATGGTTCCTTTTTCACTGAC
  GCGGATTTTGCCCAGGCAGTTGCAGCAGCCGCCGAGTATGCGGGCCTGAAGGTGGCTCGCCGCCAGATGC
  AGGATGCTGCTGGCCGCCGACACTTCCATGCCTCCCAGTGCCCTAGGCCCACGAGTCCTGTGTCTACAGA
  CAGCAACATGAGTGCTGCTGTAATCCAGAAAGCCAGACCCACCAAGAAACAGAAGCACCAGCCAGGACAT
  CTGCGCAGAGAGGCCTACACAGATGATCTTCCACCACCTCCAGTGCCACCGCCTGCTATAAAATCGCCCA
  GTGTCCAGTCCAAGGCACAGCTGGAGGCACGGCCTATAATGGGGCCAAAGCTCGCTTCTATAGAAGCGAG
  AGCAGACAGATCATCAGACAGAAAAGGCGGCAGTTACAAGGGGAGAGAAGCTCTGGACGGAAGACAAGTC
  ACTGACCTGCGAACAAGCCCAGGTGACCCCAGAGAAGCACAGGAGCAGCCAAACGAAGGGAAAGCACGAG
  GGACGAAGACAGCAAAGCGAGACCTTCCACCAGCGAAGACACACCTCATCCCAGAGGATATCCTTCCGTA
  CTGTAGGCCTACTTTCCCAACGTCAAACAACCCTAGGGATCCCAGTTCTTCAAGCTCAATGTCATCAAGA
  GGCTCAGGAAGCCGACAGAGAGAGCAAGCAAATGTGGGCCGAAGAAATATGGCAGAAATGCAAGTACTTG
  GGGGATTTGAAAGAGGAGATGAGAATAATGAAGAATTAGAGGAAACTGAAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205390 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKWKHLPLLVMISLLTLSKKHLLLAQLIPDPEDLERGNDNGTPAPTSDNDDNSLGYTGSRLRQEDFPPRI
  VEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRP
  DEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSP
  LDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESKVADVTVLERPSFVKRPSNLAVTVDDSAE
  FKCEARGDPVPTFGWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEP
  PHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTVTNVQR
  SDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLTLSCVATGSPVPTILWRK
  DGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLI
  PSAPSKPEVTDVSKNTVTLLWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIY
  LFLVRAANAYGISDPSQISDPVKTQDVPPTTQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQ
  SQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKT
  LEERPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIP
  FLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWII
  LMVFSIWLYRHRKKRNGLSSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTW
  PNTGNSHNDCSINCCTASNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEM
  KTFNSPNLKDGRFVNPSGQPTPYATTQLIQANLINNMNNGGGDSSEKHWKPPGQQKQEVAPIQYNIMEQN
  KLNKDYRANDTILPTIPYNHXYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPP
  AHPPPHSNSEEYSMSVDESYDQEMPCPVPPARMYLQQDELEEEEAERGPTPPVRGAASSPAAVSYSHQST
  ATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEE
  EDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTD
  ADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVIQKARPTKKQKHQPGH
  LRREAYTDDLPPPPVPPPAIKSPSVQSKAQLEARPIMGPKLASIEARADRSSDRKGGSYKGREALDGRQV
  TDLRTSPGDPREAQEQPNEGKARGTKTAKRDLPPAKTHLIPEDILPYCRPTFPTSNNPRDPSSSSSMSSR
  GSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022188
• ORF Size: 4953 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022188.1, NP_071524.1
• RefSeq Size: 4956 bp
• RefSeq ORF: 4956 bp
• Locus ID: 58946
• UniProt ID: O55005
• Cytogenetics: 11p11
• MW: 180.8 kDa
• Gene Summary: putative axon guidance receptor [RGD, Feb 2006]