Itga5 (NM_001108118) Rat Tagged ORF Clone

CAT#: RR205331

Itga5 (Myc-DDK-tagged ORF) - Rat integrin alpha 5 (fibronectin receptor alpha) (Itga5), (10 ug)

Product Images

Circular map for RR205331

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Itga5
• Synonyms: Itga5_retired
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205331 representing NM_001108118
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCCCCGCCAGCCGACCCGGCGGCCGGGGGGAGGGCCCAGCCGGGAGTTGGGCAAACTCCTCCCCG
  CGTTGAGTCATTCGCCTCTGGGAGGTTTAGGAAGCGGCTCCGGGTCGGTGGCCCCAGGACAGGGAAGAAC
  GGGCGCTATGGGGAGCTGGACGCCACGGTCGCCACGATCTCCTCTCCATGCGGTGCTGCTGCGCTGGGGG
  CCCCGACGCCTACCGCCGCTGCTGCCGCTGCTGCTGCTACTGTGGCCGCCACCACTCCAGGTTGGGGGCT
  TCAACCTAGACGCGGAGGCCCCGGCGGTGCTCTCCGGGCCCCCCGGCTCCCTCTTTGGCTTCTCCGTGGA
  GTTTTACCGGCCGGGAAGGGACGGAGTCAGTGTGCTGGTTGGGGCACCCAAGGCTAACACTAGCCAACCA
  GGAGTACTACAAGGTGGCGCTGTCTATGTGTGTCCCTGGGGCACCAGTCCTATCCAGTGCAGCACCATTC
  AATTTGACAGCAAAGGCTCCCGCATCCTGGAGTCTTCATTGTATAGTGAGGAACCTGTGGAGTACAAGTC
  CTTGCAGTGGTTCGGGGCAACGGTTCGGGCCCACGGCTCCTCCATCTTGGCATGCGCTCCACTGTATAGC
  TGGCGCACAGAAAAGGACCCACAGAATGACCCAGTGGGCACCTGCTACCTCTCCACAGAAAACTTCACCC
  GGATTCTGGAGTATGCACCTTGCCGCTCAGATTTTGGCAGTGCAGCAGGGCAGGGTTACTGCCAAGGGGG
  CTTCAGTGCCGAGTTCACCAAGACTGGCCGCGTGGTCCTAGGAGGACCTGGGAGCTACTTCTGGCAAGGC
  CAGATCCTGTCGGCCACTCAAGAGCAGATCTCGGAGTCCTATTACCCACAGTACCTCATCAACCCTGTTC
  AGGGGCAGCTGCAGACCCGCCAGGCCAGCTCCGTCTATGATGACAGCTACTTGGGATACTCTGTGGCTGT
  AGGTGAATTCAGTGGTGATGACACAGAAGACTTTGTTGCTGGCGTGCCCAAAGGAAACCTCACCTATGGC
  TATGTCACTGTCCTTAATGGCTCAGACATCCACTCCCTCTACAACGTCTCAGGAGAACAGATGGCCTCCT
  ATTTTGGCTATGCCGTAGCTGCCACTGATACCAATGGAGATGGGCTAGATGACCTGCTGGTAGGGGCACC
  CCTACTCATGGAGCGGACAGCTGACGGGAGACCTCAGGAGGTGGGCAGGGTCTACATCTATCTGCAGCAC
  CCAGAGGGTATAGAGCCTACGCCCTCCCTCACCCTCACTGGCCAAGATGAGTTCGGCCGATTCGGCAGCT
  CCTTGACACCCCTGGGGGACCTGGACCAAGACGGCTACAATGATGTCGCCATTGGGGCTCCGTTTGGTGG
  GGAGGCCCAGCAAGGAGTTGTATTTATATTCCCGGGAGGCCCAGGAGGACTGAACACTAAGCCTTCCCAG
  GTTTTGCAGCCTCTGTGGGCAGCTGGCCATACCCCAGACTTCTTTGGCTCTGCCCTCCGAGGAGGACGAG
  ATCTGGACGGCAATGGATACCCTGATCTAATCGTTGGATCCTTTGGTGTGGACAAGGCTCTGGTATACAG
  GGGTCGGCCCATCATATCTGCCAGTGCGTCTCTCACCATCTTCCCCTCCATGTTCAACCCAGAGGAGCGC
  AGCTGCAGCCTGGAAGGAAACCCTGTATCCTGCATCAACCTTAGCTTCTGCCTCAACGCCTCTGGAAAAC
  ATGTACCCAACTCTATAGGCTTCGAGGTGGAACTCCAACTGGACTGGCAGAAGCAGAAGGGAGGGGTCCG
  GAGGGCACTGTTCCTGGCTTCCAAGCAGGCCACCCTTACCCAGACCCTGCTTATCCAGAATGGGGCTCGG
  GAGGACTGCAGGGAGATGAAGATCTACCTCAGGAATGAATCAGAATTCAGAGACAAACTCTCCCCAATTC
  ACATTGCCCTCAACTTCTCCTTGGACCCCAAAGCCCCCATGGACAGCCATGGCCTCCGGCCAGTCCTACA
  CTACCAGAGCAAGAGCCGGATAGAGGACAAGGCCCAGATCTTGCTAGACTGTGGTGAAGACAACATCTGT
  GTGCCTGACCTGCAGCTGGCTGTGTATGGGGAGAAGAAACATGTGTACCTGGGTGACAAGAACGCCCTGA
  ACCTGACATTCCTCGCCCAGAATCTGGGCGAGGGTGGGGCCTATGAAGCCGAGCTGCGGGTCACAGCCCC
  CCTGGAGGCTGAGTACTCGGGGCTTGTCAGACACCCAGGGAACTTCTCCAGCCTGAGCTGTGACTACTTC
  GCGGTGAACCAGAGCCGCCAGCTGGTGTGTGACCTGGGCAACCCCATGAAGGCAGGCACCAGTATCTGGG
  GTGGCCTTCGGTTCACTGTTCCTCATCTTCAAGACACAAAGAAAACCATCCAATTTGACTTCCAGATCCT
  CAGCAAGAACCTGAACAACTCACAAAGCAACATGGTCTCCTTCCCACTCTCGGTGGAGGCTCAAGCCCAA
  GTCTCCCTTAACGGTGTCTCCAAGCCTGAAGCCGTGATTTTCCCAGTCAGCGACTGGAATCCTCAAGACC
  AGCCTCAGAAGGAGGGAGATTTGGGCCCTGCTGTCCACCATGTCTATGAGCTCATTAACCAGGGGCCCAG
  CTCCATCAGCCAGGGTGTGCTGGAGATCAGCTGTCCCCAGGCTCTGGAAGGCCAACAGCTCCTCTATGTG
  ACCAAGGTGACGGGACTCAACAACTGCACCTCCAACTACACCCCCAACTCACAGGGCCTGGAGTTGGATC
  CAGAGGTTTCTCCACATCACCTGCAGAGGCGAGAGGCTCCAGGGAGGAGTTCTACCACCTCAGGAACACA
  AGTCCTGAAATGCCCTGAAGCCAAGTGTTTCAGGCTGCGCTGTGAGTTTGGGCCACTGCATCGCCAAGAG
  AGCCGGAGTCTGCAGCTGCATTTCCGAGTCTGGGCCAAGACCTTCTTGCAGCAGGAATACCAGCCGTTCA
  GCCTTCAGTGTGAGGCTCTATATGAAGCCCTGAAGATGCCCTACCAAATCCTGCCTCGGCAGCTGCCCCA
  AAAGAAACTCCAGGTGGCCACAGCCGTGCAGTGGACCAAGGCAGAAGGCAGCAATGGCGTCCCACTGTGG
  ATCATCATCCTCGCCATCCTTTTTGGCCTCCTGCTCCTAGGTCTGCTCATCTATGTCCTCTACAAGCTCG
  GCTTCTTCAAACGCTCCCTCCCCTACGGCACAGCCATGGAAAAAGCTCAGCTCAAGCCTCCAGCCACCTC
  AGATGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205331 representing NM_001108118
  Red=Cloning site Green=Tags(s)
  
  MPPRQPTRRPGGGPSRELGKLLPALSHSPLGGLGSGSGSVAPGQGRTGAMGSWTPRSPRSPLHAVLLRWG
  PRRLPPLLPLLLLLWPPPLQVGGFNLDAEAPAVLSGPPGSLFGFSVEFYRPGRDGVSVLVGAPKANTSQP
  GVLQGGAVYVCPWGTSPIQCSTIQFDSKGSRILESSLYSEEPVEYKSLQWFGATVRAHGSSILACAPLYS
  WRTEKDPQNDPVGTCYLSTENFTRILEYAPCRSDFGSAAGQGYCQGGFSAEFTKTGRVVLGGPGSYFWQG
  QILSATQEQISESYYPQYLINPVQGQLQTRQASSVYDDSYLGYSVAVGEFSGDDTEDFVAGVPKGNLTYG
  YVTVLNGSDIHSLYNVSGEQMASYFGYAVAATDTNGDGLDDLLVGAPLLMERTADGRPQEVGRVYIYLQH
  PEGIEPTPSLTLTGQDEFGRFGSSLTPLGDLDQDGYNDVAIGAPFGGEAQQGVVFIFPGGPGGLNTKPSQ
  VLQPLWAAGHTPDFFGSALRGGRDLDGNGYPDLIVGSFGVDKALVYRGRPIISASASLTIFPSMFNPEER
  SCSLEGNPVSCINLSFCLNASGKHVPNSIGFEVELQLDWQKQKGGVRRALFLASKQATLTQTLLIQNGAR
  EDCREMKIYLRNESEFRDKLSPIHIALNFSLDPKAPMDSHGLRPVLHYQSKSRIEDKAQILLDCGEDNIC
  VPDLQLAVYGEKKHVYLGDKNALNLTFLAQNLGEGGAYEAELRVTAPLEAEYSGLVRHPGNFSSLSCDYF
  AVNQSRQLVCDLGNPMKAGTSIWGGLRFTVPHLQDTKKTIQFDFQILSKNLNNSQSNMVSFPLSVEAQAQ
  VSLNGVSKPEAVIFPVSDWNPQDQPQKEGDLGPAVHHVYELINQGPSSISQGVLEISCPQALEGQQLLYV
  TKVTGLNNCTSNYTPNSQGLELDPEVSPHHLQRREAPGRSSTTSGTQVLKCPEAKCFRLRCEFGPLHRQE
  SRSLQLHFRVWAKTFLQQEYQPFSLQCEALYEALKMPYQILPRQLPQKKLQVATAVQWTKAEGSNGVPLW
  IIILAILFGLLLLGLLIYVLYKLGFFKRSLPYGTAMEKAQLKPPATSDA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001108118
• ORF Size: 3297 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108118.1, NP_001101588.1
• RefSeq Size: 4395 bp
• RefSeq ORF: 3300 bp
• Locus ID: 315346
• Cytogenetics: 7q36
• MW: 119.5 kDa
• Gene Summary: alpha subunit that interacts with beta 1 subunit to form a fibronectin receptor [RGD, Feb 2006]