Dnm1l (NM_053655) Rat Tagged ORF Clone

CAT#: RR205234

Dnm1l (Myc-DDK-tagged ORF) - Rat dynamin 1-like (Dnm1l), (10 ug)

Product Images

Circular map for RR205234

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dnm1l
• Synonyms: DLP1; Dnml1; Drp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205234 representing NM_053655
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGCTGATCCCGGTCATCAATAAGCTGCAAGACGTCTTCAACACCGTGGGCGCCGACATCATCC
  AGCTGCCTCAGATTGTCGTAGTGGGAACTCAGAGCAGTGGAAAGAGCTCAGTGCTGGAAAGCCTAGTGGG
  GAGGGACCTTCTTCCCAGAGGGACTGGTGTGGTCACCCGGAGACCTCTTATTCTGCAGCTGGTCCACGTT
  TCACCAGAAGATAAAAGAAAAACAACAGGAGAAGAAAATGGAGTTGAAGCAGAAGAATGGGGTAAATTTC
  TTCACACCAAAAACAAGCTTTACACAGATTTTGATGAAATTCGACAAGAAATTGAAAATGAAACTGAAAG
  AATTTCAGGAAATAATAAGGGAGTAAGCCCTGAGCCAATCCATCTCAAGGTTTTCTCGCCCAACGTTGTC
  AACCTGACGCTTGTGGATTTACCAGGAATGACCAAAGTACCTGTAGGTGATCAGCCCAAGGATATTGAGC
  TTCAAATCAGAGAACTTATTCTTCGGTTCATCAGTAATCCCAATTCCATTATCCTTGCTGTCACTGCTGC
  AAATACAGATATGGCAACGTCAGAGGCACTTAAGATTTCCAGAGAGGTAGATCCAGATGGGCGCAGAACT
  CTAGCTGTAATCACCAAGCTTGATCTCATGGATGCGGGTACTGATGCCATGGATGTATTGATGGGAAGGG
  TTATTCCAGTCAAGCTTGGAATAATTGGAGTAGTTAACAGGAGCCAACTGGACATTAACAATAAGAAGAG
  TGTAACTGATTCAATCCGTGATGAGTATGCTTTTCTTCAAAAGAAGTACCCATCTCTGGCCAACAGAAAT
  GGAACAAAGTATCTTGCTAGGACTCTGAATAGGTTACTTATGCATCATATCAGAGACTGTTTACCAGAGC
  TGAAAACAAGAATAAATGTCTTAGCTGCTCAGTATCAGTCTCTTCTAAATAGCTATGGTGAGCCCGTGGA
  TGATAAAAGTGCTACTTTACTCCAGCTCATTACCAAGTTTGCCACAGAGTATTGTAACACTATTGAAGGA
  ACGGCAAAGTACATTGAAACTTCTGAGCTATGTGGTGGTGCTAGGATTTGTTATATTTTCCATGAGACTT
  TTGGGCGAACCTTAGAATCTGTTGATCCACTAGGTGGCCTTAACACTATTGACATCTTGACCGCCATTAG
  AAATGCTACTGGCCCCCGTCCAGCTTTATTTGTGCCTGAAGTTTCATTTGAGTTACTGGTCAAACGTCAG
  ATTAAGCGTCTAGAGGAGCCCAGCCTCCGCTGTGTGGAGCTGGTCCACGAGGAAATGCAGAGGATCATTC
  AGCACTGTAGCAACTACAGCACACAGGAACTGTTACGGTTCCCTAAACTCCATGATGCCATAGTTGAAGT
  AGTGACTTGTCTTCTTCGTAAAAGGTTGCCCGTGACAAATGAAATGGTACATAACTTAGTGGCAATTGAG
  CTAGCATATATCAACACAAAACACCCCGACTTTGCTGATGCCTGTGGGCTAATGAACAATAACATAGAGG
  AACAAAGAAGAAACAGACTAGCAAGAGAACTACCTTCCGCTGTATCGCGAGACAAGTTAATTCAGGACAA
  CAGAAGAGAAACTAAAAATGTTGCATCTGCAGGTGGTGGAATTGGAGATGGTGGTCGAATTGGAGATGGT
  GGTCAGGAACCGACAACAGGCAACTGGAGAGGAATGCTGAAAACTTCAAAAGCTGAAGAATTACTTGCTG
  AAGAAAAATCAAAACCAATTCCAATTATGCCAGCAAGTCCACAAAAAGGCCATGCTGTCAATTTGCTAGA
  TGTGCCAGTTCCAGTTGCACGAAAACTGTCTGCCCGAGAACAGCGAGATTGTGAGGTTATTGAAAGACTC
  ATCAAATCATATTTTCTAATTGTCAGAAAGAATATTCAAGACAGCGTCCCAAAGGCAGTAATGCATTTTT
  TGGTTAATCATGTGAAAGATACTCTTCAGAGTGAATTAGTAGGGCAGCTGTATAAGTCATCCTTATTAGA
  TGACCTTCTGACTGAATCCGAGGACATGGCACAGCGAAGAAAAGAAGCGGCTGACATGCTGAAGGCATTA
  CAAGGAGCCAGTCAAATTATTGCTGAAATCCGAGAGACTCATCTTTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205234 representing NM_053655
  Red=Cloning site Green=Tags(s)
  
  MEALIPVINKLQDVFNTVGADIIQLPQIVVVGTQSSGKSSVLESLVGRDLLPRGTGVVTRRPLILQLVHV
  SPEDKRKTTGEENGVEAEEWGKFLHTKNKLYTDFDEIRQEIENETERISGNNKGVSPEPIHLKVFSPNVV
  NLTLVDLPGMTKVPVGDQPKDIELQIRELILRFISNPNSIILAVTAANTDMATSEALKISREVDPDGRRT
  LAVITKLDLMDAGTDAMDVLMGRVIPVKLGIIGVVNRSQLDINNKKSVTDSIRDEYAFLQKKYPSLANRN
  GTKYLARTLNRLLMHHIRDCLPELKTRINVLAAQYQSLLNSYGEPVDDKSATLLQLITKFATEYCNTIEG
  TAKYIETSELCGGARICYIFHETFGRTLESVDPLGGLNTIDILTAIRNATGPRPALFVPEVSFELLVKRQ
  IKRLEEPSLRCVELVHEEMQRIIQHCSNYSTQELLRFPKLHDAIVEVVTCLLRKRLPVTNEMVHNLVAIE
  LAYINTKHPDFADACGLMNNNIEEQRRNRLARELPSAVSRDKLIQDNRRETKNVASAGGGIGDGGRIGDG
  GQEPTTGNWRGMLKTSKAEELLAEEKSKPIPIMPASPQKGHAVNLLDVPVPVARKLSAREQRDCEVIERL
  IKSYFLIVRKNIQDSVPKAVMHFLVNHVKDTLQSELVGQLYKSSLLDDLLTESEDMAQRRKEAADMLKAL
  QGASQIIAEIRETHLW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053655
• ORF Size: 2148 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053655.3, NP_446107.2
• RefSeq Size: 2515 bp
• RefSeq ORF: 2151 bp
• Locus ID: 114114
• UniProt ID: O35303
• Cytogenetics: 11q23
• MW: 80 kDa
• Gene Summary: human homolog is a GTPase that is necessary for peroxisome division [RGD, Feb 2006]