Mybbp1a (NM_031668) Rat Tagged ORF Clone

CAT#: RR205222

Mybbp1a (Myc-DDK-tagged ORF) - Rat MYB binding protein (P160) 1a (Mybbp1a), (10 ug)

Product Images

Circular map for RR205222

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mybbp1a
• Synonyms: DBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205222 representing NM_031668
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATATGCCCTGAAGCGCCTAATCACTGGGCTTGGGGTGGGCCGAGAAGCTGCTAGGCCCTGCTACA
  GTCTGGCGCTAGCACAGCTGTTGCAGTCTTTTGAAGACATCCAGTTGTGTGACATCCTGGGACAGATACA
  AGAAAAATACAATCTACAAGCAATGAACAAGGCAATGATGAGACCTACTCTCTTTGCAAACCTGTTTGCA
  GTGCTTGCCCTCTTTCAGTCAGGACGCCTAGTGAAGGATAAAGAAGCCCTGATGAAGTGCGTGAGATTGC
  TGAAGATATTGTCCCATCACTACAACCACTTACAGGGGCAGCCAGTAAAGGCTCTGGTGGACATCCTCTC
  CGAGGTCCCAGAGTCCATGTTCCAGGAGATCCTGCCAAAGGTCCTCAAGGGGGACATGAAAGTGATCCTC
  AGCTCTCCCAAGTACTTGGAGCTCTTCCTCTTGGCTCGGCAGAGGGTGCCAGCAGAGCTCGAGTCACTGG
  TTGGCTCGGTTGATCTGTTCTCAGAAGACAACATTCCCAGTCTGGTGAATATACTGAAGGTGGCGGCCAA
  CTCTGTAAAGAAGGAGCAGAAGCTGCCTGATGTGGCTCTGAACCTGCTCCGCCTGGCACTTCAGGAGAAC
  AAATTTGAACGGTTCTGGAAGGAGGTTTTGGAGGAGGGGCTGCTGAAGAAGCCATCCTATACATCCAGCT
  ACATGTGTTTCCGCCTACTGGGTGCATCTCTGCCGCTTCTGTCAGATGAGCAGTTGCAGTTGGTGATGCG
  AGGAGACTTGATCCGACATTTTGGGGAGCACATGGTCGTTTCTAAGTCCCAAAACCCGCTAAGGTTCATT
  CCAGAGATAAGTGCATATGTGGGTACCTTCCTCGAGGGGTGCCAGGACGATCCCAAGCGGCAATTCACTG
  TGATGGTGGCCTTCACGGCCATCACCAATCAAGGTCTCCCTGTCATGCCTACCTTCTGGCGTGTCACGCG
  GTTCTTGAATACTGAAGCCCTGCAGAACTACGTGACCTGGCTGCGGGACATGTTCCTGCAGCCTGACCTG
  GACTCCTTGGTTGACTTCAGCACTGCCAACCAGAAGAGAGTTCAGGACGCCTCCTTGAATGTGCCTGAGC
  GAACAGTATTCCGGCTCCGGAAGTGGATCATCCACCGCCTGGTCAGCCTTGTGGATCATCTGCACCTGGA
  GAAGGATGAAGCTGTGGTCGAGCAAATAGCCAGGTTTTGCTTGTTCCATGCCTTCTTTAAGACAAAGAAA
  GCTACTCCCCAGATCCCAGAGACAAAGCAGCACTTCTCCTTCCCCTTGGAGGACGGCAACCGTGGGGTCA
  TTGTCAGTGCCTTCTTCAGCCTACTGCAGACCCTCAGTGTGAAGTTCAGACAGACACCAGACCTGGCTGA
  AAATGGGAAGCCCTGGACTTACCGCCTGGTGCAGCTGGCAGACATGCTGTTGAAGCATAACCGCAACGTA
  GCCAATGTGACACCCTTGACAGCACAGCAGCGTCAGGCCTGGGACCAGATGATGAGTACGCTGAAGGAAC
  TGGAGGCCCAGTCCTCAGAGACCCGGGCCATTGCCTTCCAGCACCTGCTGCTTCTGGTGGGCCTCCACCT
  CTTCAAGTCCCCTGCAGAAAGCTGTGACGTCCTAGGAGACATTCAGACTTGCATCAAGAAAAGCATGGAG
  CAGAATCTGCGCCGATCACGCTCTAGAGCCAAAGCCTCCCAGGAGCCAGTGTGGGTGGAGGTGATGGTGG
  AGATCTTGCTGTCCTTGCTGGCTCAACCCAGTAACCTGATGCGCCAGGTGGTCCGGAGTGTTTTTGGTCA
  TGTCTGTTCCCACCTGACTCCACGTGGGCTACAGCTAATCCTGGCTGTGCTCAACCCTGAGACAAATGAG
  GATGAGGAGGACAACGTGGTGGTCACTGACACTGACGAGAAGCAGCTGAAACATGGAGAGGACGCAGACT
  CAGACAGTGAGGACAGTAAGAACTCAGAGAGTGACGTGGATAGTGAGGATGGGGAAGAAAGTGAAGAGGA
  GGACCGTGATAAGGATGTGGACCCAGGCTTCCGACAACAGCTGATGGAAGTGTTGCAAGCTGGGAATGCA
  CTGGGTGGAGAGGAGGAGGAGGAGGAAGAGCTTGGGGATGAGGCCATGATGGCCCTGGACCAGAACCTGG
  CCAGCCTATTTGCAGAGCAGAAGATGCGTATCCAGGCCCGGCATGAGGAGAAAAACAAGCTGCAGAAGGA
  GAAGCAGCTCCGACGAGACTTCCAGATCAGGGCACTAGACCTGATTGAGGTGCTGGTGACCAAGCAGCCT
  GAGCACCCCCTGATCCTGGAACTTCTTGAGCCACTGCTAAACATCATCCAGCGCAGCATGCGCAGCAGAG
  GCTCCACCAAGCAGGAGCAGGACCTCCTGCACAAGACCGCCCGCATCTTCATGCATCATCTTTGTCGTGC
  CCGCCACTACTGCCACGAGGTGGAGCCTGGCGCAGAGGCTCTGCATGCCCAGGTGGAGAGGTTTGTGCAG
  CAGGCTGGCAACCAGGCTGACGCCTCTGTTGCCCTTTACTATTTCAATGCCTCTCTGTACCTGCTGCGAG
  TCCTCAAGGGCAACACCACTAAGAGGTACCAAGACGGTCAGAAGTTAGAGGGAGCAGACATCAAGTCTGA
  ACCCAAGGACTCAGAGGTCCAGACTACCAGTTGCTTGGACTTGGACTTTGTGACCCGGGTGTATTCAGCG
  TCATTGGAATCTCTTCTGACCAAGCGTAACAGCCCACTCACCATCCCCATGTTCCTCGACCTCTTCTCCC
  GATATCCAGTGATCTGTAAGAACTTGCTTCCCATCGTGGTCCAGCATGTGGCTGGCTCATCTCGGCCCCG
  CCATCAGGCCCAGGCGTGCCTGCTGCTCCAGAAGGCCCTGTCTGCACGGGAGCTGAGAGTGTGTTTTGAG
  GATCCTGAGTGGGAGCAGCTGATTAGCCAGGTCTTGGGAAAGACCACCCAGACCCTGCAGACTCTTGGGG
  AAGCACAGAGCAAAGGGGAACACCAGAGGGAGCTATCCATCTTGGAGCTGCTGAACACTGTCTTCAGGAT
  AGTCAATCACGAGAAGCTGTCTGTGGACCTCACTGCTTTCTTGGGCATGCTTCAAGGCAAGCAGCAGAAG
  CTGCAGCAAAACCTGCAGCAAGGGAATCACTCGTCTGGCTCCAGTCGCCTCTACGATCTCTACTGGCAGG
  CCATGAACTTGCTTGGAGTGCAGCGTCCAAAGTCAGAAAAGAAGAATGTGAAGGATATTCCTAGTGACAG
  CCAGAGTCCCATTAGCACAAAGCGGAAGAAAAAGGGATTCTTGCCAGAGACCAAGAAGCGAAAGAAACTT
  AAATCTGAGGGTACCACATCAGAAAAGAAGGCTGCATCACAGCAGGATCGAGTGACAGAGGGTGCCATGC
  CTGCTGCCACTGGTAAAGACCAGCCCCCCAGCACAGGCAAGAAAAGAAGGAAGAGAGTGAAGGCTAACAC
  CCCATCCCAGGTGAATGGAGTAACTGTGGCCAAGAGTCCGGCTCCCAATAACCCCACCCTGAGTCCCAGC
  ACCCCCCCTGCCAAGACCCCAAAAGTGCAGAAGAAAAAAGAGAAGCTGTCACAGGTGAATGGATCCACTC
  CTGTGTCCCCCGTAGAGCCTGAAAGCAAAAAGCATCAGAAGGCTCTCAGCACAAAGGAGGTCAAAAGAAG
  GTCCTCCCAGTCTGCCCTGCCAAAGAAAAGAGCAAGGCTGTCTCTGGTGAGCAGGAGCCCCAGCCTGTTA
  CAGAGTGGGATCAGGAAAAGGAGAGTGGCCAGGAGGAGAGTCCAGACACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205222 representing NM_031668
  Red=Cloning site Green=Tags(s)
  
  MKYALKRLITGLGVGREAARPCYSLALAQLLQSFEDIQLCDILGQIQEKYNLQAMNKAMMRPTLFANLFA
  VLALFQSGRLVKDKEALMKCVRLLKILSHHYNHLQGQPVKALVDILSEVPESMFQEILPKVLKGDMKVIL
  SSPKYLELFLLARQRVPAELESLVGSVDLFSEDNIPSLVNILKVAANSVKKEQKLPDVALNLLRLALQEN
  KFERFWKEVLEEGLLKKPSYTSSYMCFRLLGASLPLLSDEQLQLVMRGDLIRHFGEHMVVSKSQNPLRFI
  PEISAYVGTFLEGCQDDPKRQFTVMVAFTAITNQGLPVMPTFWRVTRFLNTEALQNYVTWLRDMFLQPDL
  DSLVDFSTANQKRVQDASLNVPERTVFRLRKWIIHRLVSLVDHLHLEKDEAVVEQIARFCLFHAFFKTKK
  ATPQIPETKQHFSFPLEDGNRGVIVSAFFSLLQTLSVKFRQTPDLAENGKPWTYRLVQLADMLLKHNRNV
  ANVTPLTAQQRQAWDQMMSTLKELEAQSSETRAIAFQHLLLLVGLHLFKSPAESCDVLGDIQTCIKKSME
  QNLRRSRSRAKASQEPVWVEVMVEILLSLLAQPSNLMRQVVRSVFGHVCSHLTPRGLQLILAVLNPETNE
  DEEDNVVVTDTDEKQLKHGEDADSDSEDSKNSESDVDSEDGEESEEEDRDKDVDPGFRQQLMEVLQAGNA
  LGGEEEEEEELGDEAMMALDQNLASLFAEQKMRIQARHEEKNKLQKEKQLRRDFQIRALDLIEVLVTKQP
  EHPLILELLEPLLNIIQRSMRSRGSTKQEQDLLHKTARIFMHHLCRARHYCHEVEPGAEALHAQVERFVQ
  QAGNQADASVALYYFNASLYLLRVLKGNTTKRYQDGQKLEGADIKSEPKDSEVQTTSCLDLDFVTRVYSA
  SLESLLTKRNSPLTIPMFLDLFSRYPVICKNLLPIVVQHVAGSSRPRHQAQACLLLQKALSARELRVCFE
  DPEWEQLISQVLGKTTQTLQTLGEAQSKGEHQRELSILELLNTVFRIVNHEKLSVDLTAFLGMLQGKQQK
  LQQNLQQGNHSSGSSRLYDLYWQAMNLLGVQRPKSEKKNVKDIPSDSQSPISTKRKKKGFLPETKKRKKL
  KSEGTTSEKKAASQQDRVTEGAMPAATGKDQPPSTGKKRRKRVKANTPSQVNGVTVAKSPAPNNPTLSPS
  TPPAKTPKVQKKKEKLSQVNGSTPVSPVEPESKKHQKALSTKEVKRRSSQSALPKKRARLSLVSRSPSLL
  QSGIRKRRVARRRVQTP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031668
• ORF Size: 3831 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031668.1, NP_113856.1
• RefSeq Size: 3834 bp
• RefSeq ORF: 3834 bp
• Locus ID: 60571
• UniProt ID: O35821
• Cytogenetics: 10q24
• MW: 144.7 kDa
• Gene Summary: transcriptional activator that displays a circadian rhythm of expression in hepatocytes [RGD, Feb 2006]