Adrb3 (NM_013108) Rat Tagged ORF Clone

CAT#: RR205205

Adrb3 (Myc-DDK-tagged ORF) - Rat adrenergic, beta-3-, receptor (Adrb3), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adrb3
• Synonyms: ADRB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205205 representing NM_013108
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCGTGGCCTCACAAAAACGGCTCTCTGGCTTTCTGGTCAGACGCCCCCACCTTGGACCCCAGTG
  CAGCCAACACCAGTGGGTTGCCAGGGGTGCCATGGGCAGCGGCATTGGCTGGAGCATTGCTGGCGCTGGC
  CACGGTGGGAGGCAACCTGCTGGTAATCACAGCTATCGCCCGCACGCCGAGACTACAGACCATAACCAAC
  GTGTTCGTGACTTCGCTGGCCACAGCTGACTTGGTAGTGGGACTCCTCGTAATGCCACCAGGGGCCACAT
  TGGCGCTGACTGGCCACTGGCCCTTGGGCGCAACTGGCTGCGAGCTGTGGACGTCAGTGGACGTGCTCTG
  TGTAACTGCCAGCATCGAGACCCTGTGCGCCCTGGCTGTAGACCGCTACCTAGCCGTCACCAACCCTCTG
  CGTTACGGCACGCTGGTTACCAAGCGCCGCGCCCGGGCGGCAGTAGTCCTGGTGTGGATCGTGTCCGCCA
  CCGTGTCCTTTGCGCCCATCATGAGCCAGTGGTGGCGTGTAGGGGCAGACGCTGAGGCGCAAGAGTGTCA
  CTCCAATCCGCGCTGCTGTTCCTTTGCCTCCAATATGCCCTACGCGCTGCTCTCCTCCTCCGTCTCCTTC
  TACCTTCCCCTCCTTGTGATGCTCTTCGTCTATGCTCGAGTGTTCGTCGTAGCTAAGCGCCAGCGGCGTT
  TGCTGCGCCGGGAGCTGGGCCGTTTTCCGCCCGAGGAGTCTCCGCGGTCTCCGTCGCGCTCTCCATCCCC
  TGCCACAGTCGGGACACCCACGGCATCGGATGGAGTGCCCTCCTGCGGGCGGCGGCCTGCGCGCCTCCTA
  CCGCTCGGGGAACACCGCGCCCTGCGCACCTTGGGTCTCATTATGGGCATCTTCTCTCTGTGCTGGCTGC
  CCTTCTTTCTGGCCAACGTGCTGCGCGCACTCGTGGGGCCCTCCCTAGTTCCCAGCGGAGTTTTCATCGC
  CCTGAACTGGTTGGGCTATGCCAACTCTGCCTTCAACCCGCTCATCTACTGCCGCAGCCCGGACTTTCGC
  GACGCCTTCCGTCGTCTTCTGTGCAGCTACGGTGGCCGTGGACCGGAAGAGCCACGCGTGGTCACCTTCC
  CAGCTAGCCCTGTTGCGTCCAGGCAGAACTCACCGCTCAACAGGTTTGATGGCTATGAAGGTGAGCGTCC
  ATTTCCCACA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205205 representing NM_013108
  Red=Cloning site Green=Tags(s)
  
  MAPWPHKNGSLAFWSDAPTLDPSAANTSGLPGVPWAAALAGALLALATVGGNLLVITAIARTPRLQTITN
  VFVTSLATADLVVGLLVMPPGATLALTGHWPLGATGCELWTSVDVLCVTASIETLCALAVDRYLAVTNPL
  RYGTLVTKRRARAAVVLVWIVSATVSFAPIMSQWWRVGADAEAQECHSNPRCCSFASNMPYALLSSSVSF
  YLPLLVMLFVYARVFVVAKRQRRLLRRELGRFPPEESPRSPSRSPSPATVGTPTASDGVPSCGRRPARLL
  PLGEHRALRTLGLIMGIFSLCWLPFFLANVLRALVGPSLVPSGVFIALNWLGYANSAFNPLIYCRSPDFR
  DAFRRLLCSYGGRGPEEPRVVTFPASPVASRQNSPLNRFDGYEGERPFPT
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013108
• ORF Size: 1200 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013108.2, NP_037240.2
• RefSeq Size: 2185 bp
• RefSeq ORF: 1203 bp
• Locus ID: 25645
• UniProt ID: P26255
• Cytogenetics: 16q12.3
• MW: 43.1 kDa
• Gene Summary: binds BRL373 and norepenephrine; may play a role in the adrenergic signaling pathway [RGD, Feb 2006]