Taldo1 (NM_031811) Rat Tagged ORF Clone

CAT#: RR205161

Taldo1 (Myc-DDK-tagged ORF) - Rat transaldolase 1 (Taldo1), (10 ug)

Product Images

Circular map for RR205161

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Taldo1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205161 representing NM_031811
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGGGTCCCCGGTAAAACGCCAGAGGATGGAGTCCGCCTTGGACCAGCTCAAGCAGTTCACCACCG
  TGGTGGCTGACACGGGTGATTTCAACGCCATCGATGAGTACAAGCCCCAGGATGCCACCACCAACCCATC
  CCTGATCCTGGCTGCAGCACAGATGCCTGCCTACCAAGAGCTGGTGGAGGAGGCCATTGCCTACGGCAAG
  AAGCTGGGTGGGCCACAAGAGGAGCAGATTAAAAATGCCATTGATAAACTTTTTGTGCTGTTTGGGGCAG
  AAATACTAAAGAAGATTCCAGGCCGTGTATCCACAGAAGTCGATGCAAGGCTTTCCTTTGATAAGGATGC
  CATGGTGGCCCGAGCCAGGCGCATCATAGAGCTTTACAAAGAAGCTGGGATCAGCAAGGACAGAATTCTC
  ATCAAGTTATCATCAACCTGGGAGGGAATCCAGGCCGGAAAGGAGCTGGAGGAGCAGCATGGCATCCACT
  GCAACATGACACTGCTTTTCTCCTTCGCCCAGGCCGTGGCCTGCGCTGAAGCGGGCGTGACGCTCATCTC
  TCCCTTTGTGGGGCGCATCCTTGACTGGCATGTGGCAAACACAGACAAGAAATCCTACGAACCCCAGGAG
  GACCCTGGGGTGAAGAGTGTCACAAAAATCTACAACTACTACAAAAAGTTTGGCTACAAGACCATTGTCA
  TGGGTGCTTCCTTCCGTAACACGGGTGAGATCAAAGCGCTGGCAGGCTGTGATTTCCTCACCATCTCACC
  CAAGCTTCTGGGGGAGCTGCTCAAGGACAGCAGCAAGCTGGCACCCACGCTTTCCGTCAAAGCAGCCCAG
  ACCAGTGACTTGGAGAAGATACATCTGGACGAGAAGGCCTTCCGTTGGCTGCACAATGAGGACCAAATGG
  CTGTGGAGAAGCTCTCTGATGGGATCCGCAAGTTTGCTGCTGATGCCATAAAGTTGGAGCGGATGCTCAC
  AGAACGAATGTTCAGCGCTGAGAATGGGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205161 representing NM_031811
  Red=Cloning site Green=Tags(s)
  
  MSGSPVKRQRMESALDQLKQFTTVVADTGDFNAIDEYKPQDATTNPSLILAAAQMPAYQELVEEAIAYGK
  KLGGPQEEQIKNAIDKLFVLFGAEILKKIPGRVSTEVDARLSFDKDAMVARARRIIELYKEAGISKDRIL
  IKLSSTWEGIQAGKELEEQHGIHCNMTLLFSFAQAVACAEAGVTLISPFVGRILDWHVANTDKKSYEPQE
  DPGVKSVTKIYNYYKKFGYKTIVMGASFRNTGEIKALAGCDFLTISPKLLGELLKDSSKLAPTLSVKAAQ
  TSDLEKIHLDEKAFRWLHNEDQMAVEKLSDGIRKFAADAIKLERMLTERMFSAENGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031811
• ORF Size: 1011 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031811.3, NP_113999.2
• RefSeq Size: 1225 bp
• RefSeq ORF: 1014 bp
• Locus ID: 83688
• UniProt ID: Q9EQS0
• Cytogenetics: 1q41
• MW: 37.5 kDa
• Gene Summary: human homolog is a pentose phosphate pathway enzyme involved in generation of reducing equivalents to protect cellular integrity from reactive oxygen intermediates [RGD, Feb 2006]