Acvrl1 (NM_022441) Rat Tagged ORF Clone

CAT#: RR205137

Acvrl1 (Myc-DDK-tagged ORF) - Rat activin A receptor type II-like 1 (Acvrl1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acvrl1
• Synonyms: R-3; R3; SETHKIR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205137 representing NM_022441
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCTGGGGATTTTCCGAAGGGTCTTTTTGATGCTGTCGGTGGCCTTGGGCCTAACTAAGGGAGACT
  TGGTGAAGCCCTCCAGGGGTCAGCTGGTAAACTGCACTTGTGAGAACCCACACTGCAAGAGGCCAATCTG
  CCAGGGGGCATGGTGCACAGTGGTGCTAGTTCGAGAGCAGGGCAGGCACCCCCAGGTCTATCGGGGCTGC
  GGGAGCCTGAACCAGGAGCTCTGCCTGGGACGTCCCACGGAGTTTGTGAACCATCACTGCTGCTATAGAT
  CCTTCTGCAACCACAATGTGTCCCTGATGCTGGAGGCCACCCAAACTCCTTCGGAGGAGCCAGAAGTAGA
  TGCCCATCTGCCTCTGATCCTGGGTCCCGTGCTGGCCTTGCTGGTCCTGGTGGCCCTGGGCACTCTGGGC
  TTGTGGCGTGTCCGGAGAAGGCAGGAGAAGCAGCGGGGTCTGCACAGTGACCTGGGCGAGTCCAGTCTCA
  TCCTGAAGGCATCGGAACAGGGAGACAGCATGTTGGGGGACTTCCTGGTCAGCGACTGTACCACAGGCAG
  CGGCTCAGGGCTACCCTTCTTGGTGCAGAGGACAGTAGCGCGACAGGTTGCACTGGTGGAGTGTGTGGGA
  AAGGGCCGATATGGCGAGGTGTGGCGCGGTTCGTGGCATGGCGAGAGTGTGGCGGTCAAGATTTTCTCCT
  CACGAGATGAGCAGTCCTGGTTCCGGGAGACAGAGATCTACAACACAGTTCTGCTTAGACACGACAACAT
  CCTAGGCTTCATCGCCTCCGACATGACCTCGCGGAACTCCAGCACGCAGCTGTGGCTTATCACCCACTAC
  CACGAGCATGGCTCCCTCTATGACTTTCTGCAGAGGCAGACGCTGGAGCCCCAGTTGGCCCTGAGGCTGG
  CTGTGTCCGCGGCCTGCGGCCTGGCGCACCTGCATGTAGAGATCTTTGGCACTCAAGGCAAACCAGCCAT
  CGCCCATCGTGACCTCAAGAGCCGCAACGTGCTGGTCAAGAGCAACTTGCAGTGTTGCATTGCAGACCTG
  GGATTGGCTGTGATGCACTCGCAAAGCAGCGATTACCTGGACATTGGTAACAACCCCCGAGTGGGTACCA
  AGAGATACATGGCACCCGAGGTGCTGGATGAGCAGATCCGCACAGACTGTTTTGAGTCGTACAAGTGGAC
  AGACATCTGGGCCTTCGGCTTAGTGCTATGGGAGATTGCCCGGCGGACCATCATCAATGGCATTGTGGAG
  GACTACAGGCCACCCTTCTATGACATGGTACCCAATGACCCCAGTTTTGAGGACATGAAAAAGGTGGTGT
  GTGTTGACCAGCAGACCCCCACCATCCCTAACCGACTGGCAGCAGATCCGGTCCTCTCCGGGCTGGCCCA
  GATGATGCGAGAGTGCTGGTACCCCAACCCCTCCGCTCGCCTCACCGCACTGCGCATAAAGAAGACATTA
  CAGAAGCTCAGCCAGAATCCAGAGAAACCCAAAGTGATTCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205137 representing NM_022441
  Red=Cloning site Green=Tags(s)
  
  MTLGIFRRVFLMLSVALGLTKGDLVKPSRGQLVNCTCENPHCKRPICQGAWCTVVLVREQGRHPQVYRGC
  GSLNQELCLGRPTEFVNHHCCYRSFCNHNVSLMLEATQTPSEEPEVDAHLPLILGPVLALLVLVALGTLG
  LWRVRRRQEKQRGLHSDLGESSLILKASEQGDSMLGDFLVSDCTTGSGSGLPFLVQRTVARQVALVECVG
  KGRYGEVWRGSWHGESVAVKIFSSRDEQSWFRETEIYNTVLLRHDNILGFIASDMTSRNSSTQLWLITHY
  HEHGSLYDFLQRQTLEPQLALRLAVSAACGLAHLHVEIFGTQGKPAIAHRDLKSRNVLVKSNLQCCIADL
  GLAVMHSQSSDYLDIGNNPRVGTKRYMAPEVLDEQIRTDCFESYKWTDIWAFGLVLWEIARRTIINGIVE
  DYRPPFYDMVPNDPSFEDMKKVVCVDQQTPTIPNRLAADPVLSGLAQMMRECWYPNPSARLTALRIKKTL
  QKLSQNPEKPKVIH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022441
• ORF Size: 1512 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022441.2, NP_071886.1
• RefSeq Size: 3540 bp
• RefSeq ORF: 1515 bp
• Locus ID: 25237
• UniProt ID: P80203
• Cytogenetics: 7q36
• MW: 57.2 kDa
• Gene Summary: may play a role in TGF-beta mediated transmembrane signaling in blood vessels [RGD, Feb 2006]