Snx20 (NM_001024999) Rat Tagged ORF Clone

CAT#: RR204824

Snx20 (Myc-DDK-tagged ORF) - Rat sorting nexin 20 (Snx20), (10 ug)

Product Images

Circular map for RR204824

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Snx20
• Synonyms: RGD1307787; Slic1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204824 representing NM_001024999
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAGTCCACAGCATCCTGGGGGCCCTGGCTGGACAGGACCCAGAAACCAATGCATAACAAGGACCA
  GACAGGAAGTCCTGCCCCCAGGTCCAGACCTCCCATGTCCAGGCCCAGAAGAAGCCCAAGATGGTCCCAC
  CTCCAACTCCAACATGACCACAAGGGAGCTACAGGAACACTGGCAGAAGGAAAAGAGCCGCTGGAAACAC
  GTCAGGCTCCTCTTTGAGATCGCCTCAGCCAGAATCGAGGAGAGGAAAGTCTCCAAGTTTGTGATGTACC
  AGGTCGTGGTCATCCAGACTGGGAGCTTCGACAGCGACAAGGCTGTGGTGGAGCGGCGCTACTCGGACTT
  CGAGAGGCTGCAGAGGGCCCTCCTGAAGCGCTTCGGGCCGGAGCTGGAGGACGTGACGTTCCCGCGGAAG
  CGCCTGACCGGGAACCTGTCGGCCGAGACCATCTGCGAACGGCGCCTGGAGCTGCGCGAGTACCTGCGGC
  TGCTGTACGCCGTGCGCGCCGTGCGCCGCTCGCGCGAGTTCGCCGACTTCCTTACGCGGCCCGAGCTGTG
  CGAGGCCTTCGGCTGCCTGCGCGCCGGCCAGTACGCGCGCGCGCTCGACCTGCTGGGCCGCGTGGTGCCG
  CTGCAGGAGAAGCTCACGGCGCACTGCCCGAGCGCTCCCGTGCCTGCTCTCTGCGCCATGCTCGTGTGCC
  TGCGCGACCTGGAGCGGCCGGCCGAGGCCTTCGCGGTCGGCGAGCGCGCGCTGCGACGCCTGGGGGCGCG
  CGAGAGCCACCGCTACTACGCGCCGCTGCTGGACGCCATGGTGCGCCTGGCCTACGCGCTGGGCAAGGAC
  TTGGCGTCGCTGCAGGGCCGGCTGGATGAGAGCCAGCTCCGGAGGCCCACACACAGGGGCGCCACCCTGA
  AGGAGCTCACCGTGCGGGAGTATCTGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204824 representing NM_001024999
  Red=Cloning site Green=Tags(s)
  
  MASPQHPGGPGWTGPRNQCITRTRQEVLPPGPDLPCPGPEEAQDGPTSNSNMTTRELQEHWQKEKSRWKH
  VRLLFEIASARIEERKVSKFVMYQVVVIQTGSFDSDKAVVERRYSDFERLQRALLKRFGPELEDVTFPRK
  RLTGNLSAETICERRLELREYLRLLYAVRAVRRSREFADFLTRPELCEAFGCLRAGQYARALDLLGRVVP
  LQEKLTAHCPSAPVPALCAMLVCLRDLERPAEAFAVGERALRRLGARESHRYYAPLLDAMVRLAYALGKD
  LASLQGRLDESQLRRPTHRGATLKELTVREYLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024999
• ORF Size: 939 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024999.1, NP_001020170.1
• RefSeq Size: 1393 bp
• RefSeq ORF: 942 bp
• Locus ID: 307742
• UniProt ID: Q5BK61
• Cytogenetics: 19p11
• MW: 35.7 kDa
• Gene Summary: May play a role in cellular vesicle trafficking. Has been proposed to function as a sorting protein that targets SELPLG into endosomes, but has no effect on SELPLG internalization from the cell surface, or on SELPLG-mediated cell-cell adhesion.[UniProtKB/Swiss-Prot Function]