Ndufv1 (NM_001006972) Rat Tagged ORF Clone

CAT#: RR204770

Ndufv1 (Myc-DDK-tagged ORF) - Rat NADH dehydrogenase (ubiquinone) flavoprotein 1 (Ndufv1), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR204770

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ndufv1
• Synonyms: MGC94599
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204770 representing NM_001006972
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTAGCAGCACGGCATTTTCTCGGCGGGTCGGTTCCCGTGCGGGTATCTGTGCGTCTCAGCAGCGGCA
  CGACAGCACCCAAGAAAACCTCATTTGGCTCGCTGAAGGATGAAGACCGGATCTTCACCAACCTGTATGG
  GCGCCATGACTGGAGGCTGAAGGGTGCCCAGAGGCGGGGTGATTGGTACAAGACAAAGGAGATCCTGCTG
  AAGGGGCCTGACTGGATCTTGGGTGAAATGAAGACATCGGGTTTACGGGGCCGTGGTGGTGCTGGCTTCC
  CCACTGGCCTCAAATGGAGCTTTATGAATAAGCCCTCGGATGGCAGGCCCAAGTACCTGGTGGTGAATGC
  TGACGAGGGTGAGCCCGGCACCTGTAAGGACCGAGAGATCATGCGCCATGACCCTCACAAGCTGGTGGAA
  GGCTGCCTGGTGGGAGGCCGGGCCATGGGGGCCCGGGCTGCCTATATCTACATCCGAGGGGAATTCTACA
  ATGAGGCCTCCAATTTGCAGGTAGCTATCCGAGAGGCCTATGAAGCAGGTCTGATTGGCAAAAATGCCTG
  TGACTCTGATTATGATTTTGATGTGTTTGTGGTGCGTGGGGCTGGGGCCTACATCTGTGGAGAAGAGACT
  GCGCTTATTGAGTCCATTGAAGGCAAGCAGGGAAAGCCACGCCTGAAGCCGCCCTTTCCAGCAGATGTGG
  GAGTATTTGGATGCCCCACAACTGTGGCCAATGTGGAGACGGTGGCTGTGTCCCCCACCATCTGCCGTCG
  TGGCGGCACCTGGTTTGCTGGCTTTGGCCGAGAACGCAACTCAGGTACCAAACTGTTCAACATCTCTGGC
  CATGTCAACCACCCCTGCACTGTGGAGGAAGAGATGTCTGTGCCACTCAAAGAACTGATTGAGAAACATG
  CTGGTGGTGTCTTAGGTGGCTGGGACAACCTCCTTGCTGTGATTCCTGGTGGCTCATCCACTCCACTGAT
  CCCCAAATCTGTGTGTGAGACAGTGCTGATGGACTTTGATGCCCTGGTGCAGGCTCAGACGGGCCTGGGC
  ACAGCTGCGGTTATTGTTATGAACAGCTCGACAGACATTGTGAAAGCCATCGCTCGTCTCATTGAGTTCT
  ACAAGCATGAGAGCTGTGGCCAGTGCACCCCGTGCCGGGAGGGCGTTGATTGGATGAACAAGGTGATGGC
  CCGATTTGTGAAGGGGGATGCCCGGCCAGCTGAGATCGACTCCCTGTGGGAGATCAGCAAGCAGATAGAA
  GGCCACACCATTTGTGCTCTGGGTGATGGGGCCGCCTGGCCAGTACAGGGTCTGATCCGACATTTCCGGC
  CCGAGCTTGAGGATCGGATGCAACGATTTGCCCAGCAGCACCAGGCCAGGCAGGCGGCCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204770 representing NM_001006972
  Red=Cloning site Green=Tags(s)
  
  MLAARHFLGGSVPVRVSVRLSSGTTAPKKTSFGSLKDEDRIFTNLYGRHDWRLKGAQRRGDWYKTKEILL
  KGPDWILGEMKTSGLRGRGGAGFPTGLKWSFMNKPSDGRPKYLVVNADEGEPGTCKDREIMRHDPHKLVE
  GCLVGGRAMGARAAYIYIRGEFYNEASNLQVAIREAYEAGLIGKNACDSDYDFDVFVVRGAGAYICGEET
  ALIESIEGKQGKPRLKPPFPADVGVFGCPTTVANVETVAVSPTICRRGGTWFAGFGRERNSGTKLFNISG
  HVNHPCTVEEEMSVPLKELIEKHAGGVLGGWDNLLAVIPGGSSTPLIPKSVCETVLMDFDALVQAQTGLG
  TAAVIVMNSSTDIVKAIARLIEFYKHESCGQCTPCREGVDWMNKVMARFVKGDARPAEIDSLWEISKQIE
  GHTICALGDGAAWPVQGLIRHFRPELEDRMQRFAQQHQARQAAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001006972
• ORF Size: 1392 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001006972.1, NP_001006973.1
• RefSeq Size: 1506 bp
• RefSeq ORF: 1395 bp
• Locus ID: 293655
• Cytogenetics: 1q43
• MW: 50.7 kDa