Trip13 (NM_001011930) Rat Tagged ORF Clone

CAT#: RR204503

Trip13 (Myc-DDK-tagged ORF) - Rat thyroid hormone receptor interactor 13 (Trip13), (10 ug)

Product Images

Circular map for RR204503

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Trip13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204503 representing NM_001011930
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGACGCGGTGGGCGACCTGAAGCAAGCGCTTCCGTGTGTTGCCGAGTCGCCCGCGGTCCATGTGG
  AGGTTTTGCAGCGCAGCGGAAGCACTGCAAAAAAAGAAGATATAAAGCAGAGCGTTTACAGGCTGCTCAA
  GAGGCATAACATTGTGTTTGGAGATTACGTGTGGACTGAGTTTGATGAGCCTTTTCTAACTAGAAATGTT
  CAGTCGGTATCTATTGTTGACACAGAATTAAAGGCTAAAGACCCTCAGCCCATTGATCTGAGTGCATGCA
  CCATTGCACTTCACATCTTCCAGCTGAATGAAGAAGGCCCCAGCAGTGAAAATTTGGATGAAGAAACAGA
  AAATATAATTGCAGCAAGTCACTGGGTTTTGCCTGCAGCTGAATTTCATGGGCTTTGGGATAGCCTCGTG
  TATGATGTGGAGGTCAAATCACATCTCCTTGACTATGTGATGACCACCTTACTATTCTCAGACAAGAATG
  TGGACAGCAACCTTATCACCTGGAACCGGGTGGTACTGCTGCACGGTCCTCCGGGTACTGGAAAGACATC
  CCTTTGTAAGGCATTAGCCCAGAAACTGACCATCAGACTGTCAAGCAGGTACCGGTATGGCCAGTTAATT
  GAAATAAACAGCCACAGCCTATTTTCTAAGTGGTTTTCAGAAAGTGGCAAGTTGGTAACTAAGATGTTCC
  AGAAGATTCAGGACTTGATTGATGATAAAGAAGCTTTGGTGTTTGTTCTGATTGATGAGGTGGAGAGTCT
  TACAGCTGCTCGAAATGCTTGCAGGGCAGGCGCAGAGCCATCAGATGCTATCCGTGTAGTCAATGCTGTG
  TTGACTCAGATTGATCAGATTAAAAGGCATTCCAATGTGGTGATTCTGACCACTTCCAACATCACTGAGA
  AGATTGATGTGGCCTTTGTGGATAGAGCTGACATCAAACAATATATTGGCCCCCCCTCTGCAGCAGCCAT
  CTTCAAAATCTACCTGTCTTGTTTAGAAGAACTGATGAAGTGCCAGATCATATATCCCCGTCAGCAGCTG
  TTGACCCTTCGAGAGCTGGAAATGATTGGCTTCATTGAAAATAATGTGTCGAAGTTGAGCCTCCTTTTGA
  GTGAAATTTCAAGGAAGAGTGAGGGCCTCAGCGGCCGGGTCTTGAGGAAACTTCCTTTCCTGGCTCATGC
  GCTGTATATCCAGGCTCCCAGCGTCACCATCGAGGGTTTCCTCCAGGCCCTGTCACTGGCAGTGGACAAA
  CAGTTTGAGGAGAAAAAGAAACTCTCAGCTCATGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204503 representing NM_001011930
  Red=Cloning site Green=Tags(s)
  
  MDDAVGDLKQALPCVAESPAVHVEVLQRSGSTAKKEDIKQSVYRLLKRHNIVFGDYVWTEFDEPFLTRNV
  QSVSIVDTELKAKDPQPIDLSACTIALHIFQLNEEGPSSENLDEETENIIAASHWVLPAAEFHGLWDSLV
  YDVEVKSHLLDYVMTTLLFSDKNVDSNLITWNRVVLLHGPPGTGKTSLCKALAQKLTIRLSSRYRYGQLI
  EINSHSLFSKWFSESGKLVTKMFQKIQDLIDDKEALVFVLIDEVESLTAARNACRAGAEPSDAIRVVNAV
  LTQIDQIKRHSNVVILTTSNITEKIDVAFVDRADIKQYIGPPSAAAIFKIYLSCLEELMKCQIIYPRQQL
  LTLRELEMIGFIENNVSKLSLLLSEISRKSEGLSGRVLRKLPFLAHALYIQAPSVTIEGFLQALSLAVDK
  QFEEKKKLSAHV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001011930
• ORF Size: 1296 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001011930.1, NP_001011930.1
• RefSeq Size: 1821 bp
• RefSeq ORF: 1299 bp
• Locus ID: 292206
• UniProt ID: Q5XHZ9
• Cytogenetics: 1p11
• MW: 48.4 kDa
• Gene Summary: Plays a key role in chromosome recombination and chromosome structure development during meiosis. Required at early steps in meiotic recombination that leads to non-crossovers pathways. Also needed for efficient completion of homologous synapsis by influencing crossover distribution along the chromosomes affecting both crossovers and non-crossovers pathways. Also required for development of higher-order chromosome structures and is needed for synaptonemal-complex formation. In males, required for efficient synapsis of the sex chromosomes and for sex body formation. Promotes early steps of the DNA double-strand breaks (DSBs) repair process upstream of the assembly of RAD51 complexes. Required for depletion of HORMAD1 and HORMAD2 from synapsed chromosomes. Plays a role in mitotic spindle assembly checkpoint (SAC) activation (By similarity).[UniProtKB/Swiss-Prot Function]