Pdia2 (NM_001105775) Rat Tagged ORF Clone

CAT#: RR204052

Pdia2 (Myc-DDK-tagged ORF) - Rat protein disulfide isomerase family A, member 2 (Pdia2), (10 ug)

Product Images

Circular map for RR204052

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pdia2
• Synonyms: Pdip
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204052 representing NM_001105775
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGATCGGTTTCTGCCAGTGTTGCTGCTCCTGCTGGGTGTCTCAGGCTCATGGGGACAGGGAGAGG
  AGCCGGGGGGTCCCCCAGAGGTGCTCTCTGAGGAGTCCATTGGGGAAGAAGTCCCCGAGGAGGATGGAAT
  CTTGGTTTTGAACAACCAAACCCTGAGCCTGGCACTGCAGGAGCATTCAGCCCTGATGGTGGAGTTTTAT
  GCCCCATGGTGTGGTCACTGCAAGGCACTAGCTCCTGAGTACAGCAAGGCAGCTGCCCTGCTGGCAGCTG
  AGTCAGCTGCGGTCACCTTGGCCAAGGTGGATGGGCCTGCGGAACCAGAGCTGACCAAGGAGTTTGGAGT
  GGTAGGGTACCCTACACTCAAGTTCTTCCAGAATGGAAACCGCACAAACCCAGAAGAATACATAGGGCCC
  AAGACAGCTGAGGGCATTGCTGAGTGGCTGAGGCGGCGAGTGGGGCCCAGCGCCACACGTCTGGAGGATG
  AGGAAGGTGTCCAGGCACTGATGGCCAAATGGGATATGGTGGTCATTGGCTTCTTTCAGGATCTACAGGA
  AGAGGATGTGGCAACTTTCCTGGCCTTGGCCAGGGATGCTCTGGACATGGCCTTTGGCTTCACTGACCAG
  CCACAGCTTTTTGAGAAGTTTGGCCTCACCAAGGACACTGTGGTCCTTTTCAAGAAGTTTGATGAGGGTC
  GGGCCGACTTCCCGGTAGACAAGGAGGCTGGCCTGGACCTGGGGGACCTGTCCCGCTTCCTCATCACACA
  CAGCATGCACCTGGTCACAGAATTCAACAGCCAGACATCACCTAAGATCTTTGCAGCCAGGATCCTCAAC
  CACCTGCTGCTGTTTGTGAACCAGACACTAGCTCAGCACAGGGAGCTACTGACAGGCTTCAGGGAGGCAG
  CTCCTCCATTCCGGGGACAGGTGTTGTTTGTGATGGTGGATGTGGCCGCTGACAACAGCCATGTGCTAAA
  TTATTTTGGCCTCAAGGCTGAGGAAGCCCCCACCCTGCGTCTGATTAATGTTGAGACTACCAAGAAGTAT
  GCACCTGCAGGGGTGATACCCATCACTGCAGCTTCTGTGGCTGCATTCTGCCAGGCAGTCCTCCATGGGG
  AAGTCAAGCACTATCTCCTGAGCCAGGAGATACCCCCCGACTGGGACCAGCGGCCAGTCAAGACTCTTGT
  GAGCAAGAATTTTGAGCAGGTTGCCTTTGATGAAACCAAGAATGTGTTTGTCAAGTTCTATGCCCCATGG
  TGCAGCCACTGCAAGGAGATGGCTCCAGCCTGGGAGGCACTGGCAGAGAAGTACAGGGATCGTGAGGACA
  TTGTCATTGCTGAGATGGATGCTACAGCTAACGAGCTGGAGGCCTTCTCTGTGCATGGCTATCCCACTCT
  CAAGTTCTTCCCTGCAGGACCTGACCGAAAGATTATAGAATACAAAAGCACCAGGGACCTAGAGACATTC
  TCCAAGTTCCTGGACAGAGGAGGTGACCTGCCTGAGGAAGAATCCAAGGAGCCTGCAGTCTCTGCCCCAG
  AAGCACAACCCAATTCCACCTTGGGTCCCAAGGAGGAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204052 representing NM_001105775
  Red=Cloning site Green=Tags(s)
  
  MDDRFLPVLLLLLGVSGSWGQGEEPGGPPEVLSEESIGEEVPEEDGILVLNNQTLSLALQEHSALMVEFY
  APWCGHCKALAPEYSKAAALLAAESAAVTLAKVDGPAEPELTKEFGVVGYPTLKFFQNGNRTNPEEYIGP
  KTAEGIAEWLRRRVGPSATRLEDEEGVQALMAKWDMVVIGFFQDLQEEDVATFLALARDALDMAFGFTDQ
  PQLFEKFGLTKDTVVLFKKFDEGRADFPVDKEAGLDLGDLSRFLITHSMHLVTEFNSQTSPKIFAARILN
  HLLLFVNQTLAQHRELLTGFREAAPPFRGQVLFVMVDVAADNSHVLNYFGLKAEEAPTLRLINVETTKKY
  APAGVIPITAASVAAFCQAVLHGEVKHYLLSQEIPPDWDQRPVKTLVSKNFEQVAFDETKNVFVKFYAPW
  CSHCKEMAPAWEALAEKYRDREDIVIAEMDATANELEAFSVHGYPTLKFFPAGPDRKIIEYKSTRDLETF
  SKFLDRGGDLPEEESKEPAVSAPEAQPNSTLGPKEEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001105775
• ORF Size: 1581 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001105775.2, NP_001099245.2
• RefSeq Size: 1671 bp
• RefSeq ORF: 1584 bp
• Locus ID: 287164
• Cytogenetics: 10q12
• MW: 58.5 kDa