Tmem30b (NM_001080380) Rat Tagged ORF Clone

CAT#: RR203066

Tmem30b (Myc-DDK-tagged ORF) - Rat transmembrane protein 30B (Tmem30b), (10 ug)

Product Images

Circular map for RR203066

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tmem30b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203066 representing NM_001080380
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCTGGAGCGCCTCGGCGCGGGGCGCGCAGCAGCCCGACAACACCGCCTTCACGCAGCAGCGCCTCC
  CCGCCTGGCAGCCGCTGCTGTCGGCCGGCATCACGCTGCCGCTCTTCTTCTGCGCCGGCCTGGCGTTCAT
  CGGCCTGGGACTGGGCCTCTTCTACTCCTCCAACGGCATCCAGGAGCTGGAGTACGACTACACCGGCAAC
  CCCGGCACCGGCAACTGCTCGGTGTGCGCCGCCAAGGGCCAGGACCGCGCGCCGCCGCCCAGCTGCCAGT
  GCTCCTGGAGCTTCACGCTGCCCGAGCTCTTCCCGGGCCCCGTGTACCTCTACTACGAGCTGTCCAACTT
  TTACCAGAACAACCGGCGCTACGGCGTGTCCCGCGACGACGCTCAGCTCAGCGGCCTGGCCAGCGCGCTG
  CGCCACCCGGCCAACGAGTGCGCCCCCTACCAGTTCAGCTCCGACGGCCTGCCCATCGCGCCGTGCGGCG
  CCATTGCCAACAGCCTCTTCAACGATTCCTTCTCGCTCTGGCACCAGCGCCAGCCCGCGGACCCTTTCGT
  CGAGGTGCCGCTCGACCGCACCGCCATCGCCTGGTGGACTGACTACCACGTCAAGTTCCGCAACCCGCCG
  CTGGTGAACGGCAGCCTGGCGCTGGCCTTCAAGGGCACGGCGCCGCCGCCCAACTGGCACCGGCCGGTTT
  ATGAGCTCAGCCCGGATCCCAACAACACCGGCTTCATCAACCAGGACTTCGTGGTGTGGATGCGCACCGC
  AGCGCTGCCCACGTTCCGCAAGCTGTATGCGCGCATCCGCCAGGGCAACTACTCGGCCGGTCTTCCCCGG
  GGCACTTACCGGGTCAACATCACCTACAACTACCCCGTGCGCGCCTTCGGCGGCCACAAGCTTATCATCC
  TTAGCAACATCTCATGGATGGGTGGCAAGAACCCTTTTCTGGGCATCGCCTACCTGGTCGTCGGCTCCCT
  CTGTATCCTCATGGGCTTTGTCATGCTGGTCGTCTACATTCGCTACCAGGACCAGGATGACGACGACAAT
  GACGATGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203066 representing NM_001080380
  Red=Cloning site Green=Tags(s)
  
  MTWSASARGAQQPDNTAFTQQRLPAWQPLLSAGITLPLFFCAGLAFIGLGLGLFYSSNGIQELEYDYTGN
  PGTGNCSVCAAKGQDRAPPPSCQCSWSFTLPELFPGPVYLYYELSNFYQNNRRYGVSRDDAQLSGLASAL
  RHPANECAPYQFSSDGLPIAPCGAIANSLFNDSFSLWHQRQPADPFVEVPLDRTAIAWWTDYHVKFRNPP
  LVNGSLALAFKGTAPPPNWHRPVYELSPDPNNTGFINQDFVVWMRTAALPTFRKLYARIRQGNYSAGLPR
  GTYRVNITYNYPVRAFGGHKLIILSNISWMGGKNPFLGIAYLVVGSLCILMGFVMLVVYIRYQDQDDDDN
  DDE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080380
• ORF Size: 1059 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080380.1, NP_001073849.1
• RefSeq Size: 3076 bp
• RefSeq ORF: 1062 bp
• Locus ID: 314224
• Cytogenetics: 6q24
• MW: 39.3 kDa