Rsrc2 (NM_001014128) Rat Tagged ORF Clone

CAT#: RR202999

Rsrc2 (Myc-DDK-tagged ORF) - Rat LOC360807 (LOC360807), (10 ug)

Product Images

Circular map for RR202999

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rsrc2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202999 representing NM_001014128
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATAAGAACCAACTTCTTGTTAAAACAGGCAAGAAGACATGAATCCAAAGATAAGTCCTCTAAGAGAC
  ACAAGTCTGAGGAGCACAATGACAAAGAACATTCTTCTGACAAAGGAAGAGAGCGATTAAATTCATCTGA
  GAATGGTGAGGACAGACATAAACGCAAAGAAAGGAAGTCGTCACGGGGCAGAAGTCACTCGAGGTCTAGA
  TCTCGTGAGAGGCGCCATCGCAGTAGAAGCAGGGAAAGGAAGAAGTCTCGGTCAAGGAGTAGGGATCGGA
  AAAAATCTCGATCCAGAAGCAGAGACAGGAAGAAGTCAAGATCCAGAAGCAGGGATAGAAAACGGAGGAT
  CAGAACACGTTCCCGGTCAAGATCCAGACACAGGCATAGGACTAGAAGCAGGAGCAGATCACGGAGTCGA
  AGTCGAGATAGAAAGAAGAGAATCGAAAAACCAAGAAGATTTAGCAGAAGCCTAAGCCGGACACCTAGTC
  CGCCTCCCTTCAGAGGCAGAAACACAGCAATGGATGCCCAAGAAGCACTAGCTAGGAGATTAGAAAGGGC
  AAAGAAATTACAAGAACAACGAGAAAAGGAAATGGTTGAAAAACAAAAACAACAAGAAATGGCTGCAGCA
  GCTGCAGCCACTGGAGGTTCTGTTCTTAATGTTGCTGCACTCTTGGCATCGGGAACGCAAGTTACTCCTC
  AGATAGCTATGGCAGCTCAAATGGCAGCCCTGCAAGCTAAGGCCTTGGCAGAGACTGGCATAGCTGTGCC
  CAGCTACTATAACCCAGCAGCTGTGAATCCCATGAAATTTGCTGAGCAAGAGAAAAAGAGGAAAATGCTC
  TGGCAAGGCAAGAAAGAAGGCGACAAATCTCAGTCTGCTGAAATATGGGAAAAATTGAATTTTGGAAACA
  AGGACCAAAATGTCAAATTTAGGAAGTTAATGGGCATTAAGAGTGAAGATGAAGCCGGCTGTAGCTCTGT
  TGATGAAGAAAGTTACAAGACTTTGAAACAACAAGAAGAAGTTTTTCGAAATCTTGATGCTCAGTATGAA
  ATGGCAAGATCACAGACCCACACACAGCGAGGGATGGGTTTGGGTTTCACATCTTCAATGCGTGGAATGG
  ATACAGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202999 representing NM_001014128
  Red=Cloning site Green=Tags(s)
  
  MIRTNFLLKQARRHESKDKSSKRHKSEEHNDKEHSSDKGRERLNSSENGEDRHKRKERKSSRGRSHSRSR
  SRERRHRSRSRERKKSRSRSRDRKKSRSRSRDRKKSRSRSRDRKRRIRTRSRSRSRHRHRTRSRSRSRSR
  SRDRKKRIEKPRRFSRSLSRTPSPPPFRGRNTAMDAQEALARRLERAKKLQEQREKEMVEKQKQQEMAAA
  AAATGGSVLNVAALLASGTQVTPQIAMAAQMAALQAKALAETGIAVPSYYNPAAVNPMKFAEQEKKRKML
  WQGKKEGDKSQSAEIWEKLNFGNKDQNVKFRKLMGIKSEDEAGCSSVDEESYKTLKQQEEVFRNLDAQYE
  MARSQTHTQRGMGLGFTSSMRGMDTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014128
• ORF Size: 1128 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014128.1, NP_001014150.1
• RefSeq Size: 2095 bp
• RefSeq ORF: 1131 bp
• Locus ID: 360807
• UniProt ID: Q5PQR4
• Cytogenetics: 12q16
• MW: 43.9 kDa