Rbm17 (NM_001013058) Rat Tagged ORF Clone

CAT#: RR202892

Rbm17 (Myc-DDK-tagged ORF) - Rat RNA binding motif protein 17 (Rbm17), (10 ug)

Product Images

Circular map for RR202892

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rbm17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202892 representing NM_001013058
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCTATATGATGACCTGGGAGTGGAGACCAGTGACTCAAAAACTGAAGGCTGGTCCAAAAACTTCA
  AGCTTCTGCAGTCCCAGCTCCAGGTGAAGAAGGCAGCTCTCACTCAAGCCAAGAGCCAAAGGACAAAGCA
  AAGTACAGTCCTCGCTCCAGTCATCGACCTGAAGCGAGGAGGCTCCTCAGATGACCGACAGATCGTAGAC
  ACGCCACCTCATGTGGCAGCTGGGCTGAAGGACCCTGTGCCCAGTGGGTTCTCTGCAGGGGAAGTCCTGA
  TCCCCTTGGCTGATGAATATGACCCAATGTTTCCTAACGACTATGAGAAAGTGGTGAAGCGCCAGAGAGA
  AGAGCGGCAGAGGCAGCGGGAGCTGGAAAGACAGAAGGAAATAGAGGAAAGAGAAAAGAGGCGTAAAGAC
  AGACATGAAGCCAGTGGGTTTTCAAGACGCCCAGACCCGGACTCGGATGAGGATGAAGATTATGAGCGAG
  AGCGGAGGAAAAGAAGTATGGGGGGAGCTGCCATCGCCCCACCGACCTCTCTGGTAGAGAAAGACAAAGA
  GTTACCCCGAGATTTTCCCTATGAAGAGGACTCAAGACCTCGATCACAGTCTTCCAAAGCTGCTATTCCT
  CCCCCAGTGTATGAGGAGCCGGACAGACCAAGGTCTCCAACCGGCCCCAGCAACTCCTTCCTCGCTAACA
  TGGGTGGCACCGTGGCTCATAAGATCATGCAGAAGTATGGCTTCCGGGAAGGTCAGGGGCTGGGGAAACA
  TGAGCAAGGGCTAAGCACTGCATTGTCCGTGGAGAAGACCAGCAAGCGTGGCGGCAAGATCATTGTTGGT
  GACGCCACAGAGAAGGGCGAGTCTCAGGATGCATCCAAGAAGTCGGATTCAAATCCATTAACTGAAATTC
  TTAAGTGCCCTACTAAAGTGGTCTTGCTGAGGAACATGGTCGGTGCAGGAGAGGTCGATGAAGACTTGGA
  AGTTGAAACCAAGGAAGAATGTGAAAAATATGGCAAAGTTGGGAAATGTGTGATATTTGAGATTCCTGGT
  GCCCCTGATGATGAAGCAGTACGGATATTTCTAGAATTTGAGAGAGTCGAATCGGCAATTAAAGCTGTTG
  TCGATCTGAATGGGAGGTATTTTGGTGGACGGGTGGTTAAAGCCTGTTTCTACAATTTGGATAAATTCAG
  GGTCTTGGATCTAGCAGAGCAAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202892 representing NM_001013058
  Red=Cloning site Green=Tags(s)
  
  MSLYDDLGVETSDSKTEGWSKNFKLLQSQLQVKKAALTQAKSQRTKQSTVLAPVIDLKRGGSSDDRQIVD
  TPPHVAAGLKDPVPSGFSAGEVLIPLADEYDPMFPNDYEKVVKRQREERQRQRELERQKEIEEREKRRKD
  RHEASGFSRRPDPDSDEDEDYERERRKRSMGGAAIAPPTSLVEKDKELPRDFPYEEDSRPRSQSSKAAIP
  PPVYEEPDRPRSPTGPSNSFLANMGGTVAHKIMQKYGFREGQGLGKHEQGLSTALSVEKTSKRGGKIIVG
  DATEKGESQDASKKSDSNPLTEILKCPTKVVLLRNMVGAGEVDEDLEVETKEECEKYGKVGKCVIFEIPG
  APDDEAVRIFLEFERVESAIKAVVDLNGRYFGGRVVKACFYNLDKFRVLDLAEQV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013058
• ORF Size: 1215 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013058.1, NP_001013076.1
• RefSeq Size: 1560 bp
• RefSeq ORF: 1218 bp
• Locus ID: 291295
• Cytogenetics: 17q12.2
• MW: 45.3 kDa