Lgr5 (NM_001106784) Rat Tagged ORF Clone

CAT#: RR202738

Lgr5 (Myc-DDK-tagged ORF) - Rat leucine rich repeat containing G protein coupled receptor 5 (Lgr5), (10 ug)

Product Images

Circular map for RR202738

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lgr5
• Synonyms: Gpr49
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202738 representing NM_001106784
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACCTCCCGCGTCCGCATGCTCCTGTCCTTGCTGGCGCTGCTGCAGCTGGTGGCTGCCGGCAGCC
  CACCGAGACCGGACACGATGCCGCGGGGCTGCCCATCATACTGTCACTGTGAGCTGGATGGCAGGATGCT
  GCTCAGGGTGGACTGCTCCGACCTGGGGCTCTCGGAGCTGCCCTCCAACCTCAGCGTCTTCACCTCCTAC
  CTGGACCTCAGTATGAACAACATCAGTCAGCTACCTGCCAGTCTCCTGCATCGCCTCCGCTTCCTAGAAG
  AGTTGCGTCTTGCTGGAAATGCTTTGACACACATTCCCAAGGGAGCATTCGCAGGCCTTCACAGTCTCAA
  AGTTCTCATGCTGCAGAATAACCAGCTGAGACAGGTTCCGGAGGAAGCGCTACAGAACCTGAGAAGCCTT
  CAGTCCCTGCGCTTGGATGCCAACCACATCAGCTACGTGCCACCCAGCTGTTTCAGTGGTCTGCACTCCC
  TGAGGCACCTGTGGCTAGATGACAACGCTCTCACAGATGTCCCTGTCCAGGCTTTCAGAAGTTTATCAGC
  CCTGCAAGCCATGACCTTGGCCCTGAACAAAATACACCACATAGCAGACCATGCCTTTGGAAACCTCTCC
  AGCCTCGTGGTTCTGCATCTCCATAATAATAGAATCCACTCCCTGGGAAAGAAATGCTTTGATGGACTCC
  ACAGCCTGGAGACTTTAGATTTAAATTATAATAACCTTGACGAATTCCCCACTGCAATCAAGACACTCTC
  CAACCTCAAGGAACTAGGATTCCACAGCAACAATATCAGATCAATACCAGAGCGAGCATTCGTAGGCAAC
  CCTTCTCTTATCACAATACATTTCTATGACAATCCCATCCAGTTTGTTGGAATCTCTGCTTTTCAACACT
  TGCCTGAACTAAGAACGCTGACTTTGAATGGTGCCTCGCAAATTACTGAATTCCCTGACTTAACGGGGAC
  CGCAACCCTGGAGAGCCTGACTTTAACTGGAGCAAAGATCTCGTCTCTTCCCCAAACCGTCTGTGATCAG
  TTACCTAATCTCCAAGTGCTAGATTTGTCTTACAACCTGCTCGAAGACTTACCCAGTTTGTCGGGCTGCC
  AAAAGCTTCAGAAAATTGACCTGCGGCATAATGAAATCTATGAAATTAAAGGTGGCACTTTTCAGCAGTT
  GTTCAACCTCCGATCTCTGAACTTAGCCCGGAATAAAATTGCCATTATTCACCCCAACGCTTTCTCTACG
  TTGCCGTCTCTAATAAAGCTGGACCTATCATCCAACCTCCTTTCGTCTTTCCCTGTGACTGGGTTACACG
  GTTTAACTCACTTAAAATTAACGGGGAACCGCGCCTTGCAGAGCCTGATACCATCTGCAAACTTCCCGGA
  GCTCAAGATTATAGAAATGCCATACGCTTACCAGTGCTGTGCATTTGGGGGGTGTGAGAATGTCTATAAA
  ATTCCTAATCAATGGAACAAAGATGACAGCAGCAGTGTGGACGACCTTCGTAAGAAAGATGCTGGCTTAT
  TTCAAGTTCAAGATGAGCGGGACCTTGAAGACTTCCTACTTGACTTTGAGGAAGACCTGAAGGTTCTTCA
  CTCCGTGCAGTGCTCACCTCCCCCAGGTCCCTTCAAGCCCTGTGAGCACCTGTTTGGTAGCTGGCTGATC
  CGAATCGGGGTGTGGACCACAGCAGTCCTGGCGCTTTCCTGCAATGCCTTGGTGGCTTTCACAGTGTTCA
  GAACCCCTCTGTACATCTCTTCCATAAAACTGCTAATCGGGGTAATTGCGGTAGTGGACATACTCATGGG
  GGTCTCCAGTGCAATTCTGGCTGTCGTGGACACGTTCACTTTTGGCAGTTTTGCTCAACACGGTGCATGG
  TGGGAAGGTGGAATTGGCTGCCAAATTGTTGGTTTTTTGTCCATTTTTGCTTCCGAATCATCGGTCTTCC
  TGCTCACTCTGGCGGCACTGGAACGTGGTTTTTCTGTCAAGTGCTCTTCAAAGTTTGAAATGAAAGCTCC
  CCTTTCTAGCCTGAAAGCGATCATTTTGCTGTGTGTCCTCTTGGCCCTGACCATTGCCACCGTCCCCTTG
  CTAGGAGGCAGTGAGTACAATGCCTCTCCCCTCTGCCTGCCCTTACCCTTTGGGGAGCCCAGCACCACAG
  GCTACATGGTGGCTCTCGTATTGCTCAACTCCCTCTGTTTCCTCATAATGACCATTGCCTATACAAGGCT
  CTACTGCAGTTTGGAGAAGGGAGAGCTGGAGAACCTTTGGGACTGTTCTATGGTGAAGCACACTGCTCTG
  TTGCTCTTCACCAACTGTATCCTTTACTGCCCCGTGGCTTTCTTATCCTTCTCCTCTTTGCTAAACCTCA
  CGTTTATCAGTCCCGAAGTAATTAAATTTATACTTCTAGTGATTGTCCCACTTCCGGCATGCCTCAACCC
  CCTTCTCTACATTGTCTTCAACCCCCATTTTAAGGAGGATATGGGCAGCCTGGGAAAGCAAACCCGTTTC
  TGGACAAGAGCAAAACACCCAAGTCTGCTGTCCATTAACTCGGACGATGTTGAGAAACGGTCCTGTGACT
  CAACCCAAGCCTTAGTATCCTTTACCCACGCCAGCATAGCCTATGACTTGCCTTCCGATTCCGGGTCATC
  ACCAGCTTACCCAATGACTGAAAGCTGTCACCTCTCTTCAGTTGCATTTGTCCCATGTCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202738 representing NM_001106784
  Red=Cloning site Green=Tags(s)
  
  MDTSRVRMLLSLLALLQLVAAGSPPRPDTMPRGCPSYCHCELDGRMLLRVDCSDLGLSELPSNLSVFTSY
  LDLSMNNISQLPASLLHRLRFLEELRLAGNALTHIPKGAFAGLHSLKVLMLQNNQLRQVPEEALQNLRSL
  QSLRLDANHISYVPPSCFSGLHSLRHLWLDDNALTDVPVQAFRSLSALQAMTLALNKIHHIADHAFGNLS
  SLVVLHLHNNRIHSLGKKCFDGLHSLETLDLNYNNLDEFPTAIKTLSNLKELGFHSNNIRSIPERAFVGN
  PSLITIHFYDNPIQFVGISAFQHLPELRTLTLNGASQITEFPDLTGTATLESLTLTGAKISSLPQTVCDQ
  LPNLQVLDLSYNLLEDLPSLSGCQKLQKIDLRHNEIYEIKGGTFQQLFNLRSLNLARNKIAIIHPNAFST
  LPSLIKLDLSSNLLSSFPVTGLHGLTHLKLTGNRALQSLIPSANFPELKIIEMPYAYQCCAFGGCENVYK
  IPNQWNKDDSSSVDDLRKKDAGLFQVQDERDLEDFLLDFEEDLKVLHSVQCSPPPGPFKPCEHLFGSWLI
  RIGVWTTAVLALSCNALVAFTVFRTPLYISSIKLLIGVIAVVDILMGVSSAILAVVDTFTFGSFAQHGAW
  WEGGIGCQIVGFLSIFASESSVFLLTLAALERGFSVKCSSKFEMKAPLSSLKAIILLCVLLALTIATVPL
  LGGSEYNASPLCLPLPFGEPSTTGYMVALVLLNSLCFLIMTIAYTRLYCSLEKGELENLWDCSMVKHTAL
  LLFTNCILYCPVAFLSFSSLLNLTFISPEVIKFILLVIVPLPACLNPLLYIVFNPHFKEDMGSLGKQTRF
  WTRAKHPSLLSINSDDVEKRSCDSTQALVSFTHASIAYDLPSDSGSSPAYPMTESCHLSSVAFVPCL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001106784
• ORF Size: 2721 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001106784.1, NP_001100254.1
• RefSeq Size: 3130 bp
• RefSeq ORF: 2724 bp
• Locus ID: 299802
• UniProt ID: D4AC13
• Cytogenetics: 7q22
• MW: 100 kDa
• Gene Summary: Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development (By similarity).[UniProtKB/Swiss-Prot Function]