Vax2 (NM_022637) Rat Tagged ORF Clone

CAT#: RR202662

Vax2 (Myc-DDK-tagged ORF) - Rat ventral anterior homeobox 2 (Vax2), (10 ug)

Product Images

Circular map for RR202662

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vax2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202662 representing NM_022637
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGATGGGGGCGCGGAGCGGGACCGCGGCCCCAAGCGCCGGGAGGAGCCTGGTGGCCGCAGCGGGT
  GTCGTGGGGAGCACCGCGGAGCGGAAGATTTGCGTGCTGACACGGGTAGCACGAGTCCGAGGGAGATTGC
  TGGGACCTCCGCCTCCAGCCCTGCAGGCTCCAGGGAGAGCGGCGGGGACAGCGACGGACAACAGGCGCTC
  GGTGAGACGGACCACTGCCGCCGCATCCTGGTACGAGATGCCAAGGGGACCATTCGGGAAATTGTCCTGC
  CCAAGGGCCTGGACCTCGACCGACCCAAGAGGACCCGTACCTCCTTCACAGCAGAACAGCTGTATCGTTT
  GGAGATGGAGTTCCAGCGCTGCCAGTATGTGGTGGGCCGAGAGCGCACAGAGCTGGCCCGCCAGCTGAAC
  CTCTCTGAGACTCAGGTGAAGGTCTGGTTCCAGAACCGCCGCACCAAGCAGAAGAAGGACCAGAGCAGAG
  ACCTGGAAAAGCGGGCATCCTCCTCCGCCTTCGAGGCCTTTGCCACCTCCAACGTTCTGCGACTTCTGGA
  ACAGGGCCGGCTGCTCTCCGTGCCCAGAGCTCCCAGCCTCCTAGCACTGAGCCCTGGCCTGCCAGGTCTA
  ACCGCTGGCCACAGGGGCACCTCCTTGGGTGACCCCAGGAACTCCTCCCAACGCCTCAATCCAATGCCCT
  CAGCCTCAGCGTCGTCCCCTCTGCCACCCCCTCTGCCAGCCGTCTGCTTTTCCGCAGCTCCACTCCTGGA
  CTTGCCCGCCAGCTACAAACTGGGGTCCTCGGCTTTTGAGCCCTACAGCAGACTAGAACAACAGAAAGTA
  GGCAGCCCTGGCCAAAGTGACAAGAAAGCTGACATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202662 representing NM_022637
  Red=Cloning site Green=Tags(s)
  
  MGDGGAERDRGPKRREEPGGRSGCRGEHRGAEDLRADTGSTSPREIAGTSASSPAGSRESGGDSDGQQAL
  GETDHCRRILVRDAKGTIREIVLPKGLDLDRPKRTRTSFTAEQLYRLEMEFQRCQYVVGRERTELARQLN
  LSETQVKVWFQNRRTKQKKDQSRDLEKRASSSAFEAFATSNVLRLLEQGRLLSVPRAPSLLALSPGLPGL
  TAGHRGTSLGDPRNSSQRLNPMPSASASSPLPPPLPAVCFSAAPLLDLPASYKLGSSAFEPYSRLEQQKV
  GSPGQSDKKADI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022637
• ORF Size: 876 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022637.1, NP_072159.1
• RefSeq Size: 879 bp
• RefSeq ORF: 879 bp
• Locus ID: 64572
• UniProt ID: Q9JLZ9
• Cytogenetics: 4q34
• MW: 31.7 kDa
• Gene Summary: mouse homolog is a transcription factor involved in development of the ventral optic vesicle [RGD, Feb 2006]