Glb1 (NM_001108192) Rat Tagged ORF Clone

CAT#: RR202546

Glb1 (Myc-DDK-tagged ORF) - Rat galactosidase, beta 1 (Glb1), (10 ug)

Product Images

Circular map for RR202546

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Glb1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202546 representing NM_001108192
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCGGGTCCCCCTGTGTATGCTGCTCCCGCTCCTGGCGCTCCTGCAGTTGCTGGGCGCTGCGCACA
  GTTTCTATAATGTCTCCCAGAGGACATTTGAGCTGGACTACAAACGGGACCGCTTCCTCAAGGATGGGCA
  GCCATTCCGCTACATCTCGGGAAGCATTCACTACTTCCGGATACCCCGATTCTACTGGGAGGACCGGCTG
  CTAAAGATGAAGATGGCTGGGCTGGATGCAATCCAGACGTACGTGCCCTGGAACTTCCATGAACCCCAGC
  CAGGACAATATGACTTTTCTGGGGACCGTGATGTGGAACATTTCATTCAGCTGGCTCACCAGCTGGGACT
  CCTGGTGATCCTGAGGCCTGGGCCCTACATCTGTGCAGAGTGGGACATGGGGGGCTTACCCGCTTGGTTA
  CTAGAGAAAGAATCGATCGTTCTCCGGTCTTCCGACCCAGATTACCTTGCAGCTGTGGATAAATGGCTGG
  CAGTCCTTCTGCCCAAGATGAAGCGTCTGCTCTACCAGAACGGAGGGCCCATCATAACCGTGCAGGTTGA
  GAATGAGTACGGGTCCTACTTCGCCTGCGATTATAACTACCTGCGTTTCCTGGAGCACCGCTTCCGCTAC
  CATTTGGGTAACGACATCATTCTCTTCACCACTGACGGAGCAGCTGAAAAATTACTCAAGTGTGGGACCC
  TGCAGGACCTCTACGCCACAGTGGATTTTGGAACAACCGGCAATATCACACGAGCTTTCCTGATCCAGAG
  GAATTTTGAACCCAAAGGACCTTTGATCAATTCTGAGTTCTATACTGGCTGGTTAGACCACTGGGGTCAA
  CCCCATTCCAAAGTGAATACTAAGAAGTTGGTGGCCTCCCTCTATAACCTGCTTGCCTACGGGGCCAGCG
  TGAACTTGTACATGTTTATAGGTGGGACCAATTTTGCCTATTGGAATGGTGCCAACATGCCCTATGCCCC
  ACAGCCAACCAGCTATGACTATGACGCCCCACTGAGCGAGGCAGGAGACCTCACTGAGAAGTACTTCGCA
  GTACGAGATGTCATTCGGAAGTTTAAAGAAGTCCCAGAAGGCCCTATCCCTCCATCTACACCCAAGTTCG
  CATATGGAAAAGTTGCTCTGAGAAAGTTCAAGACGGTGACGGAAGCTCTGGGTATACTGTGTCCCAACGG
  GCCCGTGAAAAGCCTCTACCCCTTGACGTTCACTCAGGTAAAACAGTATTTTGGGTATGTGCTATACCGA
  ACGACACTTCCTCAAGATTGCAGTAACCCGAAACCCATTTTCTCTTCACCCATAAATGGTGTCCGTGATC
  GGGCCTACGTCTCTGTGGACGGGGTCCCCCAAGGAATCCTTGATCGAAACCGTATGAACGTTCTGAACAT
  TCGGGGGAAGGCCGGAGCCACGCTAGACATTCTGGTGGAGAACATGGGGCGTGTGAACTATGGCAACTCC
  ATCAAAGACTTTAAGGGTTTGATTTCCAACATGACCCTCAACTTCACTGTCCTCACCAACTGGACAATGT
  TCCCACTGGACACTGAGGCCCTGGCACGCAGCCATCTTGGGAACTGGGAGGCCGCTGATGAGGGTCACCT
  CGATGGACACTCGACCTTACGTTCTTCAAATTTCACACTCCCCACCTTTTACGTGGGCAACTTCTCCATC
  CCCTCGGGCATCCCAGACCTGCCGCAGGACACCTTCATCCAGTTTCCCGGATGGGCCAAGGGCCAAGTAT
  GGATCAATGGCTTTAACCTCGGGCGATACTGGCCCACAAAGGGCCCACAAATGACCTTGTTTGTGCCAAG
  GAACATCCTGACCACTTCAGCCCCAAACAACATCACAGTGCTAGAACTGGAGTCGTCACCCTGCAGTAAT
  GGGACCCTAGAGCTATGTACAGTAGAGTTTGTTGACACTCCGGTTATTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202546 representing NM_001108192
  Red=Cloning site Green=Tags(s)
  
  MFRVPLCMLLPLLALLQLLGAAHSFYNVSQRTFELDYKRDRFLKDGQPFRYISGSIHYFRIPRFYWEDRL
  LKMKMAGLDAIQTYVPWNFHEPQPGQYDFSGDRDVEHFIQLAHQLGLLVILRPGPYICAEWDMGGLPAWL
  LEKESIVLRSSDPDYLAAVDKWLAVLLPKMKRLLYQNGGPIITVQVENEYGSYFACDYNYLRFLEHRFRY
  HLGNDIILFTTDGAAEKLLKCGTLQDLYATVDFGTTGNITRAFLIQRNFEPKGPLINSEFYTGWLDHWGQ
  PHSKVNTKKLVASLYNLLAYGASVNLYMFIGGTNFAYWNGANMPYAPQPTSYDYDAPLSEAGDLTEKYFA
  VRDVIRKFKEVPEGPIPPSTPKFAYGKVALRKFKTVTEALGILCPNGPVKSLYPLTFTQVKQYFGYVLYR
  TTLPQDCSNPKPIFSSPINGVRDRAYVSVDGVPQGILDRNRMNVLNIRGKAGATLDILVENMGRVNYGNS
  IKDFKGLISNMTLNFTVLTNWTMFPLDTEALARSHLGNWEAADEGHLDGHSTLRSSNFTLPTFYVGNFSI
  PSGIPDLPQDTFIQFPGWAKGQVWINGFNLGRYWPTKGPQMTLFVPRNILTTSAPNNITVLELESSPCSN
  GTLELCTVEFVDTPVIG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001108192
• ORF Size: 1941 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108192.1, NP_001101662.1
• RefSeq Size: 2833 bp
• RefSeq ORF: 1944 bp
• Locus ID: 316033
• Cytogenetics: 8q32
• MW: 73.2 kDa
• Gene Summary: mutation of the human homolog causes GM1 gangliosidosis and Morquio B disease [RGD, Feb 2006]