Cacng8 (NM_080696) Rat Tagged ORF Clone

CAT#: RR202459

Cacng8 (Myc-DDK-tagged ORF) - Rat calcium channel, voltage-dependent, gamma subunit 8 (Cacng8), (10 ug)

Product Images

Circular map for RR202459

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cacng8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202459 representing NM_080696
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  CTGGAATCATTGAAACGCTGGAATGAAGAGAGGGGTTTGTGGTGCGAAAAGGGCGTTCAGGTACTACTGA
  CCACCATAGGCGCCTTTGCAGCTTTTGGCCTCATGACCATCGCCATCAGCACTGACTACTGGCTCTACAC
  AAGAGCTCTCATCTGCAACACCACCAACCTCACAGCAGGTGATGATGGACCACCCCATCGTGGGGGCAGT
  GGCTCCTCAGAGAAGAAGGACCCTGGGGGCCTCACACATTCAGGCCTCTGGCGGATATGCTGCCTGGAAG
  GGTTGAAAAGAGGTGTCTGCGTGAAGATCAACCACTTCCCGGAGGACACGGACTACGACCACGACAGCGC
  GGAGTACCTGCTCCGAGTAGTCCGGGCCTCCAGCATCTTTCCTATCCTGAGCGCCATCCTGCTGCTGCTC
  GGGGGCGTGTGCGTAGCTGCCTCTCGCGTCTACAAATCCAAAAGGAACATCATCCTGGGCGCAGGGATCC
  TGTTCGTGGCAGCAGGCCTGAGCAACATCATCGGGGTGATCGTGTACATATCGGCAAACGCGGGCGAGCC
  AGGCCCCAAGAGGGACGAGGAGAAGAAAAACCACTATTCGTATGGCTGGTCCTTCTACTTCGGCGGGCTG
  TCATTCATCCTGGCCGAGGTGATCGGCGTGCTAGCCGTCAACATCTACATCGAGCGCAGCCGCGAGGCAC
  ACTGCCAATCACGCTCGGACCTACTCAAGGCCGGCGGCGGCGCGGGCGGCAGTGGCGGGAGCGGCCCCTC
  GGCCATCCTCCGTCTGCCCAGTTACCGCTTCCGCTACCGCCGCCGCTCCCGCTCCAGCTCCCGAGGCTCC
  AGCGAGGCCTCGCCATCGCGGGATGCGTCTCCCGGCGGCCCCGGGGGCCCGGGCTTCGCCTCCACGGACA
  TCTCCATGTACACGCTCAGTCGCGACCCGTCCAAGGGCAGCGTGGCTGCGGGGCTGGCGAGCGCCGGGGG
  TGGAGGCGGCGGTGCCGGCGTGGGTGCCTACGGCGGGGCGGCCGGGGCAGCGGGGGGCGGCGGGACGGGC
  TCGGAGCGGGACCGAGGGAGCTCAGCGGGCTTCCTCACGCTGCACAACGCCTTCCCCAAGGAGGCGGCGT
  CCGGCGTCACGGTCACGGTCACCGGACCGCCCGCTGCGCCGGCGCCCGCGCCGCCCGCTCCTGCAGCGCC
  CGCGCCCGGGACGCTGTCCAAAGAGGCCGCCGCGTCCAACACCAACACGCTCAACAGGAAAACCACGCCC
  GTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202459 representing NM_080696
  Red=Cloning site Green=Tags(s)
  
  LESLKRWNEERGLWCEKGVQVLLTTIGAFAAFGLMTIAISTDYWLYTRALICNTTNLTAGDDGPPHRGGS
  GSSEKKDPGGLTHSGLWRICCLEGLKRGVCVKINHFPEDTDYDHDSAEYLLRVVRASSIFPILSAILLLL
  GGVCVAASRVYKSKRNIILGAGILFVAAGLSNIIGVIVYISANAGEPGPKRDEEKKNHYSYGWSFYFGGL
  SFILAEVIGVLAVNIYIERSREAHCQSRSDLLKAGGGAGGSGGSGPSAILRLPSYRFRYRRRSRSSSRGS
  SEASPSRDASPGGPGGPGFASTDISMYTLSRDPSKGSVAAGLASAGGGGGGAGVGAYGGAAGAAGGGGTG
  SERDRGSSAGFLTLHNAFPKEAASGVTVTVTGPPAAPAPAPPAPAAPAPGTLSKEAAASNTNTLNRKTTP
  V
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080696
• ORF Size: 1263 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080696.2, NP_542427.1
• RefSeq Size: 1266 bp
• RefSeq ORF: 1266 bp
• Locus ID: 140729
• UniProt ID: Q8VHW5
• Cytogenetics: 1q12
• MW: 43.3 kDa
• Gene Summary: Voltage-dependent calcium channels couple membrane depolarization in a number of cellular processes. These activities are regulated by distinct channels composed of the pore-forming alpha-1 subunit and the modulatory beta, alpha-2/delta and gamma subunits. The protein encoded by this gene represents one of the gamma subunits. It is an integral membrane protein that is thought to stabilize the calcium channel in an inactive (closed) state. This gene is located in a cluster with two similar gamma subunit-encoding genes on chromosome 1. The mRNA for this gene is believed to initiate translation from a non-AUG (CUG) start codon. [provided by RefSeq, Jul 2008]