Actc1 (NM_019183) Rat Tagged ORF Clone

CAT#: RR202192

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  • TrueORF®

Actc1 (Myc-DDK-tagged ORF) - Rat actin, alpha, cardiac muscle 1 (Actc1), (10 ug)



  "NM_019183" in other vectors (3)

USD 430.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name Actc1 (NM_019183) Rat Tagged ORF Clone
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Actc1
Synonyms MGC156490
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RR202192 representing NM_019183
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGTGACGACGAGGAGACCACAGCTTTGGTGTGCGACAATGGCTCCGGACTGGTGAAGGCTGGCTTTG
CGGGTGATGATGCTCCCAGAGCTGTCTTCCCATCCATCGTGGGTCGCCCACGCCACCAGGGTGTCATGGT
AGGTATGGGGCAGAAAGACTCCTATGTAGGTGACGAGGCTCAGAGCAAGCGAGGCATCCTGACTCTGAAG
TACCCCATAGAGCACGGCATTATCACCAACTGGGACGACATGGAGAAGATCTGGCACCACACCTTCTACA
ATGAGCTCCGTGTGGCCCCTGAGGAGCACCCGACCCTGCTCACTGAGGCCCCACTGAACCCCAAGGCCAA
CCGTGAGAAGATGACACAGATCATGTTTGAGACCTTCAATGTGCCTGCCATGTATGTGGCCATCCAGGCG
GTGCTGTCCCTGTACGCTTCTGGGAGAACCACAGGCATTGTTCTGGATTCTGGCGACGGTGTGACTCATA
ACGTCCCCATCTATGAGGGTTATGCCTTACCCCATGCCATCATGCGTCTGGATCTAGCTGGTCGGGACCT
CACTGACTACCTTATGAAGATCCTCACTGAGCGCGGGTACTCCTTTGTCACCACCGCTGAACGTGAAATT
GTCCGAGACATCAAAGAAAAACTGTGTTATGTCGCCCTGGATTTTGAGAATGAGATGGCCACCGCTGCAT
CTTCCTCCTCTCTGGAGAAGAGCTACGAACTTCCCGATGGCCAGGTCATCACCATTGGCAACGAGCGTTT
CCGCTGTCCTGAGACTCTCTTCCAGCCCTCTTTCATTGGTATGGAGTCTGCCGGCATTCACGAAACTACT
TATAACAGCATCATGAAGTGTGACATCGATATCCGCAAAGACCTGTACGCCAACAATGTCCTGTCTGGAG
GCACCACCATGTACCCTGGGATTGCTGATCGTATGCAAAAGGAAATAACTGCACTGGCTCCCAGCACCAT
GAAGATTAAGATTATTGCTCCCCCTGAGCGTAAATACTCTGTCTGGATTGGGGGCTCCATCCTGGCCTCT
CTGTCTACCTTCCAACAAATGTGGATCAGCAAGCAAGAGTACGATGAGGCCGGGCCCTCCATTGTCCACC
GCAAGTGCTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR202192 representing NM_019183
Red=Cloning site Green=Tags(s)

MCDDEETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLK
YPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQA
VLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREI
VRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETT
YNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILAS
LSTFQQMWISKQEYDEAGPSIVHRKCF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_019183
ORF Size 1131 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_019183.1, NP_062056.1
RefSeq Size 1489 bp
RefSeq ORF 1134 bp
Locus ID 29275
Cytogenetics 3q35
MW 42.5 kDa
Gene Summary may act as a structural component of the cytoskeleton; may play a role in muscle function [RGD, Feb 2006]

Other Versions

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