Nhej1 (NM_001014217) Rat Tagged ORF Clone

CAT#: RR202174

  • TrueORF®

Nhej1 (Myc-DDK-tagged ORF) - Rat nonhomologous end-joining factor 1 (Nhej1), (10 ug)

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


  "NM_001014217" in other vectors (3)

Reconstitution Protocol

USD 330.00

4 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

USD 198.00

Other products for "Nhej1"

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Nhej1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR202174 representing NM_001014217
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAACTAGAGCAAGGCCTGTTGATGCAGCCATGGGCATGGTTACAACTTGCTGAGAACTCACTCT
TGGCCAAGGCTTCTATCACCAAGCATGGTTATGCCTTGCTGATTTCGGATCTTCAACAGGTGTGGCATGA
ACAGGTGGACACTTTGGAGGTCAGCCAGCGAGCCAAGGAGCTGAACAAGCGCCTCACCGCTCCCCCTGCA
GCTTTTCTCCATCACTTGGATGAAGTGCTTCGCCCACTGTTTAAAGATTCTGCCCACCAGGATGCTGCTC
ACCCTAGCAAAGCCACTTTCTCCTGTGATCGTGGAGAGGAGGTATTGATCCTGAGGGTGCGGAGTGAGCT
CTCTGGTCTCCCCTTCAATTGGCATTTCCACTGTCTTCCAGCTAGTTCTTTACTGGTCTCTCAGCATTTG
ATTTGTCCTCTGATGGGTGTGAGCTTGGCATTGCACAGTCATGTGAGGGAGCTAGCAGCATTGCTTCGGA
TGAAGGACCTTGAGATCCAGGCCTACCAGGAGAGTGGGGCTGTGCTGAGCCGAGGTCGATTGAAGACAGA
GCCATTTGAAGAAAATTCTTTCTTGGAACAGTTTATGGTAGAGAAATTGCCAGAGGCATGTGCTGTTGGT
GATGGAAGGCCATTTGCCATGAATCTGCAGAGCCTGTATGTGGCAGTCACAAAGCAGCAGGTCCAAGCCA
GGCAGAAGCATAAAGGCTCTGGAGAGCCTCAGACATCAAGCAGCACCTCCCCTCAAGGAACTGATAGCCA
GCTTCAGAACCAGCCAGAACAGCAGATCTCTCCCACTCCAACCCTCTCAGAGCCTGAATGTGAGCCTATG
GCTGCTTCAGGCCCTGTGCATAGAGCTCAGCTGGTAAAGGCCAAGAGGAAGAAGCCCAGGGGACTCTTCA
GT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR202174 representing NM_001014217
Red=Cloning site Green=Tags(s)

MEELEQGLLMQPWAWLQLAENSLLAKASITKHGYALLISDLQQVWHEQVDTLEVSQRAKELNKRLTAPPA
AFLHHLDEVLRPLFKDSAHQDAAHPSKATFSCDRGEEVLILRVRSELSGLPFNWHFHCLPASSLLVSQHL
ICPLMGVSLALHSHVRELAALLRMKDLEIQAYQESGAVLSRGRLKTEPFEENSFLEQFMVEKLPEACAVG
DGRPFAMNLQSLYVAVTKQQVQARQKHKGSGEPQTSSSTSPQGTDSQLQNQPEQQISPTPTLSEPECEPM
AASGPVHRAQLVKAKRKKPRGLFS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001014217
ORF Size 912 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001014217.1, NP_001014239.1
RefSeq Size 1445 bp
RefSeq ORF 915 bp
Locus ID 363251
UniProt ID Q6AYI4
Cytogenetics 9q33
MW 33.9 kDa
Gene Summary DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary. Binds DNA in a length-dependent manner.[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.