Nhej1 (NM_001014217) Rat Tagged ORF Clone

CAT#: RR202174

Nhej1 (Myc-DDK-tagged ORF) - Rat nonhomologous end-joining factor 1 (Nhej1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nhej1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202174 representing NM_001014217
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAACTAGAGCAAGGCCTGTTGATGCAGCCATGGGCATGGTTACAACTTGCTGAGAACTCACTCT
  TGGCCAAGGCTTCTATCACCAAGCATGGTTATGCCTTGCTGATTTCGGATCTTCAACAGGTGTGGCATGA
  ACAGGTGGACACTTTGGAGGTCAGCCAGCGAGCCAAGGAGCTGAACAAGCGCCTCACCGCTCCCCCTGCA
  GCTTTTCTCCATCACTTGGATGAAGTGCTTCGCCCACTGTTTAAAGATTCTGCCCACCAGGATGCTGCTC
  ACCCTAGCAAAGCCACTTTCTCCTGTGATCGTGGAGAGGAGGTATTGATCCTGAGGGTGCGGAGTGAGCT
  CTCTGGTCTCCCCTTCAATTGGCATTTCCACTGTCTTCCAGCTAGTTCTTTACTGGTCTCTCAGCATTTG
  ATTTGTCCTCTGATGGGTGTGAGCTTGGCATTGCACAGTCATGTGAGGGAGCTAGCAGCATTGCTTCGGA
  TGAAGGACCTTGAGATCCAGGCCTACCAGGAGAGTGGGGCTGTGCTGAGCCGAGGTCGATTGAAGACAGA
  GCCATTTGAAGAAAATTCTTTCTTGGAACAGTTTATGGTAGAGAAATTGCCAGAGGCATGTGCTGTTGGT
  GATGGAAGGCCATTTGCCATGAATCTGCAGAGCCTGTATGTGGCAGTCACAAAGCAGCAGGTCCAAGCCA
  GGCAGAAGCATAAAGGCTCTGGAGAGCCTCAGACATCAAGCAGCACCTCCCCTCAAGGAACTGATAGCCA
  GCTTCAGAACCAGCCAGAACAGCAGATCTCTCCCACTCCAACCCTCTCAGAGCCTGAATGTGAGCCTATG
  GCTGCTTCAGGCCCTGTGCATAGAGCTCAGCTGGTAAAGGCCAAGAGGAAGAAGCCCAGGGGACTCTTCA
  GT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202174 representing NM_001014217
  Red=Cloning site Green=Tags(s)
  
  MEELEQGLLMQPWAWLQLAENSLLAKASITKHGYALLISDLQQVWHEQVDTLEVSQRAKELNKRLTAPPA
  AFLHHLDEVLRPLFKDSAHQDAAHPSKATFSCDRGEEVLILRVRSELSGLPFNWHFHCLPASSLLVSQHL
  ICPLMGVSLALHSHVRELAALLRMKDLEIQAYQESGAVLSRGRLKTEPFEENSFLEQFMVEKLPEACAVG
  DGRPFAMNLQSLYVAVTKQQVQARQKHKGSGEPQTSSSTSPQGTDSQLQNQPEQQISPTPTLSEPECEPM
  AASGPVHRAQLVKAKRKKPRGLFS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014217
• ORF Size: 912 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014217.1, NP_001014239.1
• RefSeq Size: 1445 bp
• RefSeq ORF: 915 bp
• Locus ID: 363251
• UniProt ID: Q6AYI4
• Cytogenetics: 9q33
• MW: 33.9 kDa
• Gene Summary: DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary. Binds DNA in a length-dependent manner.[UniProtKB/Swiss-Prot Function]