Xrcc1 (NM_053435) Rat Tagged ORF Clone

CAT#: RR202089

Xrcc1 (Myc-DDK-tagged ORF) - Rat X-ray repair complementing defective repair in Chinese hamster cells 1 (Xrcc1), (10 ug)

Product Images

Circular map for RR202089

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Xrcc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202089 representing NM_053435
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGAGATCAGCCTCCGCCACGTCGTGTCCTGCAGCAGCCAGGACTCGACCCATCGTGCCGAAAATC
  TTCTCAAGGCTGACACTTACCGGAAGTGGCGGTCAGCCAAGGCAGGAGAGAAGACCATCTCTGTGGTCCT
  ACAGTTGGAGAAGGAGGAACAGATACACAGTGTGGACATTGGAAACGATGGCTCGGCCTTCGTGGAGGTG
  CTAGTGGGCAGCTCGGCTGGAGGGGCCACAGCTGGTGAACAGGACTATGAGGTCCTTCTGGTCACCTCCT
  CTTTCATGTCCCCATCTGAGAGCCGAAGTGGCTCGAATCCCAACCGTGTTCGCATTTTTGGACCTGACAA
  GTTAGTCCGGGCAGCAGCAGAGAAGCGCTGGGACCGAGTTAAAATCGTGTGCAGCCAGCCATACAGCAAG
  GACTCACCCTACGGCCTGAGTTTTGTGAAGTTTCACAGCCCTCCAGACAAAGACGAGGCGGAGGCTCCAT
  CTCAGAAGGTGACAGTGACCAAGCTCGGCCAGTTCCGTGTGAAAGAGGAGGATGACAGTGCCAACTCCCT
  GAGGCCAGGGGCGCTCTTCTTCAATCGGATCAACAAGGCGGCTTCAGCCTCTGCAAGTGACCCGGCAGGA
  CCCAGCTATGCAGCGGCTACGCTGCAGGCCTCTAGTGCGGCCTCCTCAGCCTTGCCAGTCCCCAAGGTTG
  GGGGCAGCTCTTCCAAGCTTCAGGAGCCTCCCAAAGGGAAGAGGAAACTGGACTTGGGTCTAGAAGACAG
  CAAGCCTCCGAGCAAACCCTCAGCAGGGCCCGCTGCCCTCAAGAGACCCAAGTTGCCTGTTCCTAGCCGT
  ACCCCAGCTGCAACTCCAGCCTCTACCCCAGCACAGAAAGCCGTCCCAGGGAAGCCCCGGGGAGAAGGCA
  CAGAGCCTAGGGGAGCTCGCGCTGGACCCCAGGAGCTTGGCAAGATCCTGCAGGGGGTGGTGGTGGTGCT
  CAGTGGCTTCCAGAACCCCTTCCGTTCAGAGCTCCGGGACAAGGCCCTGGAGCTGGGGGCCAAGTATCGG
  CCAGACTGGACCCCGGACAGCACACACCTCATCTGTGCCTTTGCTAACACTCCCAAGTACAGCCAGGTCC
  TGGGCCTCGGAGGCCGTATTGTTCGTAAAGAGTGGGTGCTGGACTGTTACCGCATGCGGCGCCGGCTGCC
  CTCCCGGAGGTACCTCATGGCAGGCCTTGGATCCAGCAGTGAGGACGAGGGGGACTCTCACAGTGAGAGC
  GGTGAAGATGAAGCTCCCAAGCTTCCCCGGAAGAGGCCCCAGCCCAAAGCCAAGACCCAGGCAGCAGGAC
  CCAGCTCACCCCCGAGGCCACCAACCCCAGAAGAGACCAAAGCACCATCACCAGGACCCCAGGACAATAG
  TGACACTGATGGGGAACAGTCAGAAGGACGGGACAATGGGGCGGAAGATTCTGGGGACACCGAAGATGAA
  CTGAGGAGGGTGGCCAAGCAGAGGGAGCAAAGGCAGCCCCCAGCCCCAGAGGAGAATGGCGAGGACCCGT
  ATGCAGGCTCCACAGATGAGAACACAGACAGCGAGGCTCCCTCAGAGGCTGACCTGCCAATTCCAGAGCT
  CCCAGACTTCTTCCAGGGCAAGCACTTCTTCCTGTACGGCGAGTTCCCTGGGGATGAGAGGAGGAAGCTC
  ATCCGATATGTGACAGCTTTCAATGGTGAGCTCGAGGACTACATGAGCGACCGGGTCCAGTTTGTCATCA
  CGGCCCAGGAGTGGGATCCCAACTTTGAGGAGGCCCTGATGGAAAATCCTTCCCTGGCCTTCGTCCGGCC
  CCGCTGGATCTACAGTTGTAATGAGAAGCAGAAGTTACTCCCCCACCAGCTCTATGGGGTGGTGCCCCAG
  GCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202089 representing NM_053435
  Red=Cloning site Green=Tags(s)
  
  MPEISLRHVVSCSSQDSTHRAENLLKADTYRKWRSAKAGEKTISVVLQLEKEEQIHSVDIGNDGSAFVEV
  LVGSSAGGATAGEQDYEVLLVTSSFMSPSESRSGSNPNRVRIFGPDKLVRAAAEKRWDRVKIVCSQPYSK
  DSPYGLSFVKFHSPPDKDEAEAPSQKVTVTKLGQFRVKEEDDSANSLRPGALFFNRINKAASASASDPAG
  PSYAAATLQASSAASSALPVPKVGGSSSKLQEPPKGKRKLDLGLEDSKPPSKPSAGPAALKRPKLPVPSR
  TPAATPASTPAQKAVPGKPRGEGTEPRGARAGPQELGKILQGVVVVLSGFQNPFRSELRDKALELGAKYR
  PDWTPDSTHLICAFANTPKYSQVLGLGGRIVRKEWVLDCYRMRRRLPSRRYLMAGLGSSSEDEGDSHSES
  GEDEAPKLPRKRPQPKAKTQAAGPSSPPRPPTPEETKAPSPGPQDNSDTDGEQSEGRDNGAEDSGDTEDE
  LRRVAKQREQRQPPAPEENGEDPYAGSTDENTDSEAPSEADLPIPELPDFFQGKHFFLYGEFPGDERRKL
  IRYVTAFNGELEDYMSDRVQFVITAQEWDPNFEEALMENPSLAFVRPRWIYSCNEKQKLLPHQLYGVVPQ
  A
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053435
• ORF Size: 1893 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053435.1, NP_445887.1
• RefSeq Size: 2117 bp
• RefSeq ORF: 1896 bp
• Locus ID: 84495
• UniProt ID: Q9ESZ0
• Cytogenetics: 1q21
• MW: 68.8 kDa
• Gene Summary: plays a role in base-excision repair; displays decreased mRNA expression in early embryos treated with the anticancer alkylating agent cyclophosphamide [RGD, Feb 2006]