Pcgf6 (NM_001013154) Rat Tagged ORF Clone

CAT#: RR202077

Pcgf6 (Myc-DDK-tagged ORF) - Rat polycomb group ring finger 6 (Pcgf6), (10 ug)

Product Images

Circular map for RR202077

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pcgf6
• Synonyms: Rnf134
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202077 representing NM_001013154
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGGCTGAGACGGACGCCACGGAAAACAAGCGCGCCTCTGAGGCCAAGCGAGCCTCGGCCATGC
  TGCCACCGCCTCCGCCTCCCATCTCTCCGCCCGCCCTCATCCCGGCGCCAGCGGCCGGTGAGGAGGGCCC
  GGCGTCCCTGGCCCAGGCGGGCGCACCCGGCTGTTCTCGCTCTCGGCCCCCGGAGTTGGAGCCCGAGCGC
  AGCCTGGGCCGCTTGAGAGGCCGCTTCGAGGACTACGATGAAGAGTTGGAAGAGGATGAGGAGATGGAGG
  AGGAGGAAGAGGAGGAGGAAGAGATGAGCCACTTCTCTCTGCGTCTGGAGTCCGGACGGGCGGACTCCGA
  GGACGAGGAAGAGCGCCTGATCAACCTGGTTGAGCTGACCCCCTACATCTTGTGCTCCATTTGCAAAGGT
  TACTTGATAGATGCCACCACTATTACAGAATGTCTCCACACCTTCTGTAAAAGCTGCATTGTGAGACATT
  TTTACTATAGCAACAGATGCCCCAAATGCAACATAGTCGTCCACCAGACTCAGCCTCTTTATAATATAAG
  GTTGGACCGGCAGTTACAAGACATAGTATACAAATTAGTAGTCAATCTAGAGGAAAGAGAAAAAAAGCAA
  ATGCATGACTTCTATAAAGAAAGAGGTCTAGAAGTCCCTAAGCCTGCTGTTCCACAGCCCGTCCCTGCAA
  GCAAAGGAAGAACCAAGAAAGCCCTAGAGTCAGTGTTTCGTATTCCGCCTGAACTTGATGTGTCTTTATT
  ACTTGAGTTCATTGGTGCTAATGAAGACACAGGACATTTTAAGCCATTGGAAAAGAAGTTTGTGCGTGTT
  TCAGGAGAAGCAACTATTGGGCATGTAGAAAAATTTCTTAGAAGAAAAATGGGTCTTGATCCAGCTTGTC
  AGGTAGACATCATCTGTGGGGATCACTTACTGGAGCGGTACCAAACTCTGCGGGAAATCCGACGTGCAAT
  AGGTGACACAGCAATGCAGGATGGTCTACTTGTTCTTCATTATGGTCTTGTGGTTTCTCCTTTAAAAATA
  ACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202077 representing NM_001013154
  Red=Cloning site Green=Tags(s)
  
  MEEAETDATENKRASEAKRASAMLPPPPPPISPPALIPAPAAGEEGPASLAQAGAPGCSRSRPPELEPER
  SLGRLRGRFEDYDEELEEDEEMEEEEEEEEEMSHFSLRLESGRADSEDEEERLINLVELTPYILCSICKG
  YLIDATTITECLHTFCKSCIVRHFYYSNRCPKCNIVVHQTQPLYNIRLDRQLQDIVYKLVVNLEEREKKQ
  MHDFYKERGLEVPKPAVPQPVPASKGRTKKALESVFRIPPELDVSLLLEFIGANEDTGHFKPLEKKFVRV
  SGEATIGHVEKFLRRKMGLDPACQVDIICGDHLLERYQTLREIRRAIGDTAMQDGLLVLHYGLVVSPLKI
  T
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013154
• ORF Size: 1053 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013154.1, NP_001013172.1
• RefSeq Size: 2110 bp
• RefSeq ORF: 1056 bp
• Locus ID: 309457
• UniProt ID: Q5XI70
• Cytogenetics: 1q54
• MW: 39.7 kDa
• Gene Summary: Transcriptional repressor. May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity.[UniProtKB/Swiss-Prot Function]