Tmem158 (NM_057212) Rat Tagged ORF Clone

CAT#: RR202065

Tmem158 (Myc-DDK-tagged ORF) - Rat transmembrane protein 158 (Tmem158), (10 ug)

Product Images

Circular map for RR202065

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tmem158
• Synonyms: Ris1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202065 representing NM_057212
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTACCCCTTCTCGCCGCGCTACTGGCCGCCGCCTGCCCGCTGCCGCCGGCGCGCGGCGGGGCCACGG
  ATGCGCCCGGCCTTTCTGGGACGCCCCCAAATGCTTCGGCCAATGCTTCCTTCACCGGCGAACACTCCAC
  CCCGCGTCTGCTGGCATCGGCGGCATCCGCACCCCCAGAGCGCGCGGGCCCAGAGGAGGCTCCTGCGGCG
  CCCTGCAACATCAGCGTGCAACGGCAGATGCTGAGCTCCCTGCTCGTGCGCTGGGGCCGCCCGCGGGGCT
  TGCAGTGCGACCTGCTGCTCTTCTCTACCAACGCGCACGGCCGCGCCTTCTTTGCCGCCGCCTTCCACCG
  CGTCGGGCCGCCGCTGCTCATCGAGCATCTGGGGCTGGCGGCCGGCGGCGCGCAGCAAGACCTGCGCCTG
  TGCGTGGGCTGTGGCTGGGTGCGCGGCCGCCTGCGAGCTCCCGCCGGGGCTCCCACCGCTCTGCCTGCCT
  ACCCCGCGGCCGAACCGGGGCCGCTGTGGCTGCAGGGCGAGCCTCGTCACTTCTGCTGCCTGGACTTCAG
  CCTGGAGGAGCTGCAGGGCGAGCCGGGCTGGCGGCTGAACCGCAAGCCCATCGAGTCCACGCTGGTGGCC
  TGCTTCATGACCCTTGTCATCGTGGTGTGGAGCGTGGCCGCCCTCATCTGGCCGGTGCCCATCATTGCCG
  GCTTCTTGCCCAACGGCATGGAGCAGCGCCGGACCACCGCCGGCGCCCCCGCGGCCGCTCCTGCAGCCGT
  ACCCGCGGGGACTACCGCCGCAGCCGCCGCCGCTGCTGCAGCAGCTGCCGCGGCCGCGGCCGTCACCTCG
  GGGGTGGCTCCCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202065 representing NM_057212
  Red=Cloning site Green=Tags(s)
  
  MLPLLAALLAAACPLPPARGGATDAPGLSGTPPNASANASFTGEHSTPRLLASAASAPPERAGPEEAPAA
  PCNISVQRQMLSSLLVRWGRPRGLQCDLLLFSTNAHGRAFFAAAFHRVGPPLLIEHLGLAAGGAQQDLRL
  CVGCGWVRGRLRAPAGAPTALPAYPAAEPGPLWLQGEPRHFCCLDFSLEELQGEPGWRLNRKPIESTLVA
  CFMTLVIVVWSVAALIWPVPIIAGFLPNGMEQRRTTAGAPAAAPAAVPAGTTAAAAAAAAAAAAAAAVTS
  GVAPK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_057212
• ORF Size: 855 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_057212.1, NP_476560.1
• RefSeq Size: 999 bp
• RefSeq ORF: 858 bp
• Locus ID: 117582
• UniProt ID: Q91XV7
• Cytogenetics: 8q32
• MW: 29 kDa
• Gene Summary: receptor that binds brain injury-derived neurotrophic peptide; abolishes the protective effect of brain injury-derived neurotrophic peptide [RGD, Feb 2006]