Hspa9 (NM_001100658) Rat Tagged ORF Clone

CAT#: RR201951

Hspa9 (Myc-DDK-tagged ORF) - Rat heat shock protein 9 (Hspa9), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR201951

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hspa9
• Synonyms: Crp40; GRP-75; Hspa9a; PBP74
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201951 representing NM_001100658
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATAAGCGCCAGCAGAGCCGCGGCCGCGCGTCTCGTGGGCACCACAGCGTCCCGGAGTCCCGCCGCCG
  CCCGTCACCAGGATGGCTGGAATGGCCTTAGTCATGAGGCTTTTAGATTTGTTTCAAGAAGGGATTATGC
  GTCAGAAGCAATCAAGGGTGCAGTTGTTGGAATTGATTTGGGTACTACTAACTCCTGTGTGGCTGTTATG
  GAGGGCAAACAAGCCAAGGTCCTGGAGAACGCCGAAGGTGCCAGAACTACCCCTTCTGTGGTTGCCTTTA
  CACCAGATGGGGAGCGACTTGTTGGTATGCCAGCAAAACGACAAGCTGTCACCAACCCAAACAATACTTT
  CTATGCTACAAAGCGTCTTATTGGACGACGATATGATGACCCTGAAGTACAGAAAGACACTAAGAATGTT
  CCCTTTAAAATTGTCCGTGCCTCCAATGGTGATGCCTGGGTGGAGGCTCATGGAAAACTCTATTCTCCGA
  GTCAGATTGGAGCGTTTGTGTTGATGAAGATGAAAGAGACTGCAGAAAATTACTTGGGCCATACAGCAAA
  AAATGCTGTGATCACAGTCCCTGCTTATTTCAATGACTCACAGCGACAGGCCACTAAGGATGCTGGCCAG
  ATATCTGGGCTAAATGTGCTCCGAGTGATCAACGAGCCTACAGCTGCAGCTCTGGCTTACGGTCTGGACA
  AATCCGAAGACAAAGTCATTGCTGTATATGATTTAGGTGGAGGAACCTTTGACATTTCTATCCTGGAAAT
  TCAGAAAGGAGTGTTTGAGGTGAAATCCACCAATGGGGACACTTTCTTAGGAGGTGAAGACTTTGACCAA
  GCTTTGTTACGGCACATTGTCAAGGAGTTCAAGAGGGAGACAGGGGTTGATTTGACCAAAGACAACATGG
  CACTTCAGAGGGTTCGGGAAGCTGCTGAGAAGGCTAAATGTGAGCTCTCCTCATCTGTACAGACTGACAT
  CAACTTGCCATACCTTACAATGGATGCTTCTGGACCAAAGCATTTGAATATGAAGTTGACTCGAGCTCAG
  TTTGAAGGCATTGTCACAGATCTAATCAAGAGAACTATTGCTCCGTGTCAGAAAGCTATGCAGGATGCAG
  AAGTCAGCAAGAGTGACATAGGAGAAGTGATCCTGGTTGGTGGCATGACGAGGATGCCCAAGGTTCAGCA
  GACTGTGCAAGATCTTTTTGGCAGAGCCCCAAGTAAAGCTGTTAATCCTGATGAGGCTGTAGCCATTGGA
  GCTGCCATTCAAGGAGGTGTGTTGGCTGGTGATGTTACAGATGTGCTACTCTTGGATGTCACTCCCCTCT
  CTCTAGGTATTGAGACACTTGGAGGTGTCTTTACCAAGCTTATTAATAGGAACACCACTATTCCAACCAA
  AAAGAGCCAGGTATTTTCTACTGCTGCTGATGGACAAACTCAAGTAGAGATTAAAGTGTGTCAGGGGGAA
  AGAGAGATGGCCGGAGACAACAAACTTCTAGGACAGTTCACTTTGATTGGAATTCCCCCAGCCCCTCGTG
  GAGTGCCTCAGATTGAAGTTACATTTGACATTGATGCCAATGGGATTGTGCACGTTTCTGCCAAAGATAA
  AGGGACAGGACGTGAGCAACAGATTGTAATCCAGTCTTCTGGTGGATTAAGCAAAGATGATATTGAAAAC
  ATGGTTAAAAATGCAGAGAAGTACGCTGAAGAAGACCGGAGGAAGAAGGAACGTGTTGAAGCAGTTAATA
  TGGCTGAAGGAATTATTCATGACACAGAAACCAAGATGGAAGAATTCAAGGACCAGTTGCCTGCTGACGA
  GTGCAACAAGCTAAAGGAAGAGATTTCCAAAATGAGAGAACTCCTTGCTCGAAAGGACAGTGAAACAGGA
  GAAAACATTAGGCAGGCGGCATCTTCCCTTCAGCAGGCATCATTGAAACTCTTTGAAATGGCATATAAAA
  AGATGGCATCTGAACGGGAAGGTTCTGGAAGTTCTAGCACTGGGGAACAGAAGGAAGATCAGAAGGAAGA
  GAAACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201951 representing NM_001100658
  Red=Cloning site Green=Tags(s)
  
  MISASRAAAARLVGTTASRSPAAARHQDGWNGLSHEAFRFVSRRDYASEAIKGAVVGIDLGTTNSCVAVM
  EGKQAKVLENAEGARTTPSVVAFTPDGERLVGMPAKRQAVTNPNNTFYATKRLIGRRYDDPEVQKDTKNV
  PFKIVRASNGDAWVEAHGKLYSPSQIGAFVLMKMKETAENYLGHTAKNAVITVPAYFNDSQRQATKDAGQ
  ISGLNVLRVINEPTAAALAYGLDKSEDKVIAVYDLGGGTFDISILEIQKGVFEVKSTNGDTFLGGEDFDQ
  ALLRHIVKEFKRETGVDLTKDNMALQRVREAAEKAKCELSSSVQTDINLPYLTMDASGPKHLNMKLTRAQ
  FEGIVTDLIKRTIAPCQKAMQDAEVSKSDIGEVILVGGMTRMPKVQQTVQDLFGRAPSKAVNPDEAVAIG
  AAIQGGVLAGDVTDVLLLDVTPLSLGIETLGGVFTKLINRNTTIPTKKSQVFSTAADGQTQVEIKVCQGE
  REMAGDNKLLGQFTLIGIPPAPRGVPQIEVTFDIDANGIVHVSAKDKGTGREQQIVIQSSGGLSKDDIEN
  MVKNAEKYAEEDRRKKERVEAVNMAEGIIHDTETKMEEFKDQLPADECNKLKEEISKMRELLARKDSETG
  ENIRQAASSLQQASLKLFEMAYKKMASEREGSGSSSTGEQKEDQKEEKQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001100658
• ORF Size: 2037 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001100658.2, NP_001094128.2
• RefSeq Size: 2834 bp
• RefSeq ORF: 2040 bp
• Locus ID: 291671
• Cytogenetics: 18p12
• MW: 74.2 kDa