Ptgis (NM_031557) Rat Tagged ORF Clone

CAT#: RR201855

Ptgis (Myc-DDK-tagged ORF) - Rat prostaglandin I2 (prostacyclin) synthase (Ptgis), (10 ug)

Product Images

Circular map for RR201855

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ptgis
• Synonyms: PGIS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201855 representing NM_031557
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTGGGCCGCTCTGCTCGGCCTTCTGGCCGTGTTATTACTGTTGCTGCTGCTGCTGAGCCGCCGGC
  GCGCGCGGCGACCCGGTGAGCCTCCGTTGGACCTGGGCAGCATCCCCTGGCTGGGCCATGCCTTGGAGTT
  TGGGAAGGATGCCGCCAGCTTCCTTACCAGGATGAAGGAGAAGCACGGTGACATATTTACTGTGCTGGTG
  GGGGGCAGGTATGTCACTGTCCTCCTGGACCCACACTCTTACGACACAGTGGTGTGGGATCTGCGTACAC
  GGCTGGACTTCCACCCCTACGCCATCTTCCTCATGGAGAGGATTTTTGATCTGCAGCTTCCAAATTTCAA
  CCCCAGCGAGGAGAAGGCCAGGATGAAGCCGACGCTCATGCACAAAGACCTGCAGGCGCTCACGGAAGCC
  ATGTATACCAACCTGCGCACCGTCCTGCTGGGTGACTCCACAGAAGGAGGCAGTGGCTGGCAGGAGAAAG
  GTCTGCTTGAGTTCTCCTACAGCTCCCTGCTCAGCGCTGGCTACCTGACCCTGTATGGAGTGGAGGCCTC
  ACCACGCACCCACGAGAGTCAGGCCCTCGACCGTGACCACTCAGCCGACGTTTTCCGCACCTTCCGCCAG
  CTGGATCTGATGCTCCCCAAACTGGCTCGAGGCTCCTTGTCAGTGGGGGATAAAGACCATGCATGCAGTG
  TCAAAAGCCGCCTGTGGAAGCTGCTGTCTCCAGCCGGACTGGCCTCCAGGGCTGACCGGAGCAGCTGGTT
  GGAGAGTTACCTGAGACATCTTGAGGAGATGGGGGTATCAGAGGACATGCAGGCCCGGGCACTGGTGCTG
  CAGCTCTGGGCCACACAAGGGAATATGGGACCCACGGCTTTCTGGCTCCTCCTCTTCCTCCTCAAGAACC
  CGGAAGCCCTCGATGCTGTCCATGCAGAGCTGAAACGCATCGTCTGGCAAGCAGAGAAGCCTGTTTTACA
  GATGACCGCACTCCCGCAGAAGATTCTAGACAGCATGCCTGTGCTAGACAGTGTGCTCAATGAGACCCTC
  CGGCTCACGGCCGCCCCCTTCATCACCCGTGAGGTCATGGCAGACCTGGCCTTGCCTATGGCAGACAGGA
  GGGAATTCTCTCTTCGACGCGGTGACCGCCTTCTCCTCTTTCCTTTCCTGAGTCCCCAGAAGGACCCAGA
  AATCTACACAGAACCTGAAGTCTTTAAATACAACCGGTTCTTGAACCCAGATGGGTCAGAAAAGAAAGAT
  TTTTACAAAGATGGGAAACGGCTAAAGAATTACAACATGCCTTGGGGCGCAGGGCACAACCAGTGCCTGG
  GGAAGAGCTATGCCATCAACAGCATCAAACAGTTTGTGGTCCTGCTGCTGACTCATTTCGACCTGGAGCT
  GGTCAGTGAGGACACAGAGGTCCCGGAGTTTGACCTTAGCAGATATGGCTTTGGCCTGATGCAGCCGGAG
  GAAGATGTGCCCATCCGGTACCGTACCAGGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201855 representing NM_031557
  Red=Cloning site Green=Tags(s)
  
  MSWAALLGLLAVLLLLLLLLSRRRARRPGEPPLDLGSIPWLGHALEFGKDAASFLTRMKEKHGDIFTVLV
  GGRYVTVLLDPHSYDTVVWDLRTRLDFHPYAIFLMERIFDLQLPNFNPSEEKARMKPTLMHKDLQALTEA
  MYTNLRTVLLGDSTEGGSGWQEKGLLEFSYSSLLSAGYLTLYGVEASPRTHESQALDRDHSADVFRTFRQ
  LDLMLPKLARGSLSVGDKDHACSVKSRLWKLLSPAGLASRADRSSWLESYLRHLEEMGVSEDMQARALVL
  QLWATQGNMGPTAFWLLLFLLKNPEALDAVHAELKRIVWQAEKPVLQMTALPQKILDSMPVLDSVLNETL
  RLTAAPFITREVMADLALPMADRREFSLRRGDRLLLFPFLSPQKDPEIYTEPEVFKYNRFLNPDGSEKKD
  FYKDGKRLKNYNMPWGAGHNQCLGKSYAINSIKQFVVLLLTHFDLELVSEDTEVPEFDLSRYGFGLMQPE
  EDVPIRYRTRL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031557
• ORF Size: 1503 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031557.2, NP_113745.1
• RefSeq Size: 1777 bp
• RefSeq ORF: 1506 bp
• Locus ID: 25527
• UniProt ID: Q62969
• Cytogenetics: 3q42
• MW: 57.1 kDa
• Gene Summary: catalyzes the conversion of prostaglandin H2 to prostacyclin in prostaglandin biosynthesis [RGD, Feb 2006]