Pptc7 (NM_001107141) Rat Tagged ORF Clone

CAT#: RR201685

Pptc7 (Myc-DDK-tagged ORF) - Rat PTC7 protein phosphatase homolog (S. cerevisiae) (Pptc7), (10 ug)

Product Images

Circular map for RR201685

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pptc7
• Synonyms: RGD1310383
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201685 representing NM_001107141
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTCGGTCTTGTCGTACGGGCGGCTGGTGGCCCGCGCTGTGCTCGGCGGCCTGTCGCAGACCGACC
  CCAGGGCGGGCGGCGGCGGAGGCGGCGGCAGCGGCGACTACGGGCTGGTGACGGCGGGCTGCGGCTTCGG
  CAAGGACTTCCGTAAGGGCCTGCTCAAGAAGGGCGCGTGCTACGGGGACGACGCGTGTTTCGTGGCCCGC
  CACCGCTCGGCCGACGTGCTGGGAGTTGCGGATGGCGTTGGAGGGTGGAGAGACTATGGAGTGGACCCGT
  CTCAGTTCTCAGGGACTTTAATGCGAACATGTGAGCGGTTAGTAAAAGAAGGACGCTTTGTCCCCAGCAA
  TCCTGTTGGGATTCTCACCACGAGCTACTGTGAGTTGCTGCAAAACAAAGTACCCTTGCTGGGTAGCAGC
  ACAGCCTGCATCGTGGTGCTGGACAGAAGTAGCCACCGTCTGCACACAGCGAACCTGGGTGACTCGGGCT
  TCCTGGTGGTCCGGGGCGGCGAGGTCGTGCACCGCTCTGATGAGCAGCAGCATTACTTCAACACTCCATT
  CCAGCTCTCCATTGCTCCCCCGGAAGCTGAGGGAGTCGTCCTGAGCGACAGCCCCGATGCTGCTGACAGC
  ACATCTTTCGATGTTCAGTTAGGAGACATCATCCTGACAGCGACAGATGGGCTCTTTGACAACATGCCTG
  ATTATATGATCCTTCAGGAGCTGAAAAAACTGAAGAATTCAAATTATGAGAGCATACAGCGGACTGCAAG
  GAGCATCGCTGAGCAAGCGCACGAGCTGGCCTATGACCCAAATTACATGTCACCTTTCGCACAGTTCGCA
  TGTGACAACGGATTAAATGTGAGAGGTGGAAAACCAGATGACATCACCGTGCTCCTCTCAATAGTGGCTG
  AGTACACGGAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201685 representing NM_001107141
  Red=Cloning site Green=Tags(s)
  
  MFSVLSYGRLVARAVLGGLSQTDPRAGGGGGGGSGDYGLVTAGCGFGKDFRKGLLKKGACYGDDACFVAR
  HRSADVLGVADGVGGWRDYGVDPSQFSGTLMRTCERLVKEGRFVPSNPVGILTTSYCELLQNKVPLLGSS
  TACIVVLDRSSHRLHTANLGDSGFLVVRGGEVVHRSDEQQHYFNTPFQLSIAPPEAEGVVLSDSPDAADS
  TSFDVQLGDIILTATDGLFDNMPDYMILQELKKLKNSNYESIQRTARSIAEQAHELAYDPNYMSPFAQFA
  CDNGLNVRGGKPDDITVLLSIVAEYTD
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107141
• ORF Size: 921 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107141.1, NP_001100611.1
• RefSeq Size: 4234 bp
• RefSeq ORF: 924 bp
• Locus ID: 304488
• Cytogenetics: 12q16
• MW: 32.8 kDa