Dld (NM_199385) Rat Tagged ORF Clone

CAT#: RR201558

Dld (Myc-DDK-tagged ORF) - Rat dihydrolipoamide dehydrogenase (Dld), (10 ug)

Product Images

Circular map for RR201558

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dld
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201558 representing NM_199385
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAGCTGGAGTCGTGTGTACTGCTCCTTGGCCAAGAAAGGCCATTTCAATCGGCTGTCTCATGGCC
  TACAGGGGGCTTCTTCAGTTCCATTAAGAACTTATTCAGATCAACCAATTGATGCTGATGTGACAGTGAT
  AGGTTCTGGTCCTGGAGGATATGTTGCTGCCATCAAAGCTGCCCAGTTAGGCTTTAAGACAGTCTGCATT
  GAGAAGAATGAAACACTAGGAGGAACATGCTTGAATGTTGGTTGTATTCCTTCAAAGGCTTTATTAAATA
  ATTCTCATTATTACCATTTGGCCCATGGAAAAGATTTTGCATCTAGGGGAATTGAAATACCAGAAGTTCG
  CTTGAATTTAGAGAAGATGATGGAGCAGAAGCGTTCTGCAGTAAAAGCATTAACAGGGGGAATTGCCCAC
  TTATTCAAACAAAATAAGGTTGTTCATGTCAATGGATTTGGAAAGATAACTGGCAAGAATCAGGTTACAG
  CTACAACGGCCGATGGCAGCACTCAGGTTATTGGTACCAAAAACATCCTTATAGCTACGGGCTCAGAAGT
  TACTCCTTTTCCCGGAATCACGATTGATGAAGACACTATAGTGTCGTCTACGGGAGCTTTATCCTTAAAA
  AAAGTTCCAGAGAAGTTGGTTGTTATTGGTGCAGGAGTAATCGGTGTGGAATTGGGTTCAGTTTGGCAAA
  GACTTGGTGCAGATGTGACAGCGGTTGAATTTTTGGGTCATGTGGGTGGAATTGGAATCGACATGGAGAT
  ATCTAAAAATTTTCAACGTATTCTTCAAAAGCAAGGATTTAAGTTTAAGCTGAATACAAAAGTTACTGGT
  GCCACCAAGAAGTCAGATGGAAAAATTGATGTGTCTGTCGAAGCTGCATCTGGTGGGAAAGCTGAAGTCA
  TCACGTGTGATGTACTCTTGGTTTGCATCGGTCGACGGCCCTTTACGCAGAATTTGGGACTAGAAGAGCT
  TGGAATTGAACTAGATCCCAAAGGTAGAATTCCAGTCAATACCAGATTTCAAACTAAAATTCCAAATATC
  TTTGCTATTGGAGATGTGGTTGCTGGTCCAATGTTGGCTCACAAAGCAGAAGATGAAGGCATCATCTGTG
  TGGAAGGAATGGCGGGTGGTGCTGTGCACATTGATTATAATTGTGTGCCTTCAGTGATTTACACACACCC
  CGAGGTTGCTTGGGTTGGCAAATCAGAAGAACAGTTGAAAGAAGAGGGTGTCGAGTTCAAAGTTGGAAAA
  TTCCCATTTGCTGCAAACAGCAGAGCTAAAACAAATGCTGACACAGATGGCATGGTCAAGATTCTTGGAC
  ATAAATCCACAGATAGAATACTGGGAGCACATATTCTAGGACCAGGTGCTGGAGAAATGGTGAATGAAGC
  CGCCCTGGCATTGGAATATGGAGCGTCCTGTGAAGACGTAGCTAGAGTCTGCCATGCACATCCGACCTTA
  TCAGAGGCTTTTAGAGAAGCAAACTTGGCTGCGTCTTTTGGCAAACCAATCAACTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201558 representing NM_199385
  Red=Cloning site Green=Tags(s)
  
  MQSWSRVYCSLAKKGHFNRLSHGLQGASSVPLRTYSDQPIDADVTVIGSGPGGYVAAIKAAQLGFKTVCI
  EKNETLGGTCLNVGCIPSKALLNNSHYYHLAHGKDFASRGIEIPEVRLNLEKMMEQKRSAVKALTGGIAH
  LFKQNKVVHVNGFGKITGKNQVTATTADGSTQVIGTKNILIATGSEVTPFPGITIDEDTIVSSTGALSLK
  KVPEKLVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGIGIDMEISKNFQRILQKQGFKFKLNTKVTG
  ATKKSDGKIDVSVEAASGGKAEVITCDVLLVCIGRRPFTQNLGLEELGIELDPKGRIPVNTRFQTKIPNI
  FAIGDVVAGPMLAHKAEDEGIICVEGMAGGAVHIDYNCVPSVIYTHPEVAWVGKSEEQLKEEGVEFKVGK
  FPFAANSRAKTNADTDGMVKILGHKSTDRILGAHILGPGAGEMVNEAALALEYGASCEDVARVCHAHPTL
  SEAFREANLAASFGKPINF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199385
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199385.2, NP_955417.1
• RefSeq Size: 2610 bp
• RefSeq ORF: 1530 bp
• Locus ID: 298942
• UniProt ID: Q6P6R2
• Cytogenetics: 6q16
• MW: 54 kDa
• Gene Summary: This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. A similar protein in human, mouse, and pig has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, the protein can function as a protease. [provided by RefSeq, Jan 2014]