Fcnb (NM_053634) Rat Tagged ORF Clone

CAT#: RR201483

Fcnb (Myc-DDK-tagged ORF) - Rat ficolin B (Fcnb), (10 ug)

Product Images

Circular map for RR201483

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fcnb
• Synonyms: Fcn2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201483 representing NM_053634
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCTGGGATCTGCTGCACTATTCGTCTTGAGCCTGTGTGTCACTGAGCTGACCCTCCATGCTGCTG
  ACACCTGCCCAGAGGTGAAGGTCCTGGATCTGGAGGGCTCCAACAAACTCACCATCCTTCAAGGCTGCCC
  TGGCTTGCCTGGAGCCCTGGGGCCCAAGGGAGAGGCTGGTGCCAAAGGAGATAGAGGAGAGAGTGGCCTT
  CCTGGACACCCTGGAAAGGCGGGGCCAACTGGACCCAAAGGAGACCGAGGAGAGAAGGGAGTGCGTGGAG
  AAAAAGGAGACACCGGGCCCTCCCAGTCATGTGCTACAGGACCTCGGACCTGCAAGGAGTTGCTCACCCG
  GGGCTACTTTCTCACTGGCTGGTATACCATCTACCTGCCAGACTGCAGGCCCCTGACCGTGCTGTGTGAT
  ATGGACACAGATGGTGGAGGCTGGACCGTCTTTCAGAGAAGGATCGACGGCACGGTGGACTTCTTTCGGG
  ACTGGACCTCATACAAGCAAGGCTTCGGCAGCCAGCTGGGGGAGTTCTGGCTGGGGAACGATAACATCCA
  TGCTTTAACCACCCAGGGAACCAATGAGCTGCGGGTGGATCTTGCAGACTTCGACGGCAACCATGACTTT
  GCCAAGTACAGCTCCTTCCAGATCCAGGGAGAGGCCGAGAAATACAAGCTCATTCTGGGGAACTTCCTCG
  GTGGTGGTGCTGGTGACTCCCTGACTTCCCAAAACAACATGCTTTTCTCCACCAAAGACCAAGACAACGA
  CCAGGGATCCTCCAACTGTGCCGTGCGTTACCATGGAGCCTGGTGGTACTCTGATTGTCACACTTCCAAC
  CTGAATGGCCTCTACCTTAGGGGTCTCCATAAGAGCTATGCAAACGGTGTCAATTGGAAGTCATGGAAAG
  GGTACAACTACAGCTACAAGGTTTCTGAAATGAAGGTGCGCCTCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201483 representing NM_053634
  Red=Cloning site Green=Tags(s)
  
  MVLGSAALFVLSLCVTELTLHAADTCPEVKVLDLEGSNKLTILQGCPGLPGALGPKGEAGAKGDRGESGL
  PGHPGKAGPTGPKGDRGEKGVRGEKGDTGPSQSCATGPRTCKELLTRGYFLTGWYTIYLPDCRPLTVLCD
  MDTDGGGWTVFQRRIDGTVDFFRDWTSYKQGFGSQLGEFWLGNDNIHALTTQGTNELRVDLADFDGNHDF
  AKYSSFQIQGEAEKYKLILGNFLGGGAGDSLTSQNNMLFSTKDQDNDQGSSNCAVRYHGAWWYSDCHTSN
  LNGLYLRGLHKSYANGVNWKSWKGYNYSYKVSEMKVRLI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053634
• ORF Size: 957 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053634.1, NP_446086.1
• RefSeq Size: 1127 bp
• RefSeq ORF: 960 bp
• Locus ID: 114091
• UniProt ID: P57756
• Cytogenetics: 3p12
• MW: 34.7 kDa
• Gene Summary: mouse homolog is similar to mouse plasma ficolin Fcna [RGD, Feb 2006]