Kcnt1 (NM_021853) Rat Tagged ORF Clone

CAT#: RR201402

Kcnt1 (Myc-DDK-tagged ORF) - Rat potassium channel, subfamily T, member 1 (Kcnt1), (10 ug)

Product Images

Circular map for RR201402

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnt1
• Synonyms: rSlo2; Slack
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201402 representing NM_021853
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCGGGCCAAGCTGCCGCGCTCACCGTCCGAGGGCAAGGCGGGTCCGGGGGACACCCCAGCCGGCT
  CTGCAGCCCCTGAGGAGCCGCACGGACTCAGCCCGCTACTGCCCACCCGCGGCGGGGGCTCCGTGGGCAG
  CGACGTGGGCCAGAGGCTTCATGTGGAAGATTTCAGCCTGGACTCTTCCCTTTCTCAGGTCCAGGTGGAA
  TTCTATGTCAATGAGAACACCTTCAAAGAACGCCTCAAGCTGTTCTTCATCAAAAACCAGAGATCCAGCC
  TGAGGATCCGGCTGTTCAACTTCTCCCTCAAGCTCCTCACCTGCCTGCTGTACATTGTCCGTGTCCTGCT
  CGACAACCCAGACCAGGGCATCGGATGCTGGGGCTGCACGAAGTATAACTACACGTTCAATGGTTCGTCC
  TCGGAGTTCCACTGGGCTCCCATCCTGTGGGTGGAGAGGAAAATGGCTCTGTGGGTGATCCAGGTCATTG
  TGGCCACAATAAGCTTCTTAGAGACCATGCTCCTCATTTACCTCAGCTACAAAGGCAACATCTGGGAGCA
  GATATTCCATGTGTCTTTCGTCTTGGAGATGATCAACACACTGCCCTTCATCATCACGGTCTTCTGGCCA
  CCTCTCCGGAACCTGTTCATCCCTGTGTTTCTCAACTGCTGGCTGGCCAAGCACGCCCTGGAGAACATGA
  TCAATGACTTCCACCGTGCCATCCTGCGCACACAGTCAGCCATGTTCAACCAGGTGCTCATCCTGTTCTG
  CACCCTGCTGTGCCTGGTCTTCACAGGGACCTGTGGGATTCAGCACTTAGAGCGGGCAGGTGGCAACTTG
  AACTTGCTGACCTCCTTCTACTTCTGCATTGTGACTTTCTCAACCGTGGGCTTCGGTGATGTGACACCCA
  AGATCTGGCCATCCCAGCTCCTGGTGGTCATCCTGATCTGTGTCACCCTTGTGGTGCTCCCACTGCAGTT
  TGAAGAGCTTGTCTACCTCTGGATGGAGCGGCAGAAGTCAGGGGGCAACTATAGCCGCCACCGAGCACGG
  ACAGAGAAGCATGTGGTCCTGTGTGTGAGCTCCCTCAAGATCGATCTCCTCATGGATTTCCTGAATGAGT
  TCTATGCCCACCCCCGTCTCCAGGACTACTACGTGGTCATCCTGTGTCCCTCGGAAATGGATGTCCAGGT
  GCGCAGGGTCCTACAGATTCCCCTGTGGTCCCAGCGTGTCATCTACCTCCAGGGCTCTGCCCTCAAGGAC
  CAGGACCTCATGCGAGCTAAGATGGACAACGGAGAGGCCTGCTTTATCCTCAGCAGCAGGAATGAGGTGG
  ACCGCACAGCTGCGGATCACCAGACCATCCTTCGAGCCTGGGCTGTGAAAGACTTTGCCCCCAACTGCCC
  CCTCTATGTCCAGATTCTCAAGCCCGAAAACAAGTTTCACGTCAAATTTGCTGACCATGTGGTGTGCGAG
  GAAGAGTGCAAGTACGCCATGCTGGCCCTGAACTGCATCTGCCCGGCCACCTCCACCCTCATCACCCTGC
  TGGTGCACACATCCCGTGGCCAGGAAGGACAGGAGTCACCAGAGCAGTGGCAGCGCATGTACGGGCGCTG
  CTCGGGCAACGAGGTGTACCACATTCGCATGGGTGACAGTAAATTCTTCCGGGAGTATGAGGGCAAGAGC
  TTCACCTACGCAGCCTTCCACGCGCACAAGAAGTATGGGGTGTGCCTCATTGGGCTGAAACGTGAAGAGA
  ACAAGAGTATCCTGCTGAACCCGGGGCCACGGCACATCCTGGCCGCCTCTGACACCTGCTTCTATATCAA
  TATTACCAAGGAGGAGAACTCAGCTTTCATCTTCAAGCAGGAGGAAAAGCAGAATAGGCGGGGCCTGGCA
  GGGCAGGCACTTTACGAAGGGCCCTCCCGGCTCCCAGTGCACAGCATCATCGCCTCTATGGTGGCCATGG
  ACCTGCAGAACACAGATTGCCGGCCCTCCCAGGGTGGCAGTGGTGGGGGCGGCGGCAAGCTGACTCTGCC
  CACTGAGAACGGCTCTGGCAGTCGGCGTCCCAGCATCGCACCCGTTCTGGAGTTGGCAGACAGCTCAGCC
  CTGTTGCCCTGCGACCTGCTGAGTGACCAATCAGAGGATGAGGTGACACCCTCAGACGACGAGGGGCTCT
  CTGTGGTTGAGTATGTGAAGGGCTACCCTCCCAACTCACCCTACATTGGCAGCTCCCCGACTTTATGCCA
  CCTCTTGCCTGTAAAGGCCCCCTTCTGCTGCCTGCGACTGGATAAGGGCTGCAAACACAACAGCTATGAG
  GATGCCAAGGCCTACGGGTTCAAGAACAAGTTGATCATCGTCTCTGCTGAGACGGCAGGCAACGGGCTCT
  ACAACTTCATCGTGCCTCTGCGTGCCTACTACCGGTCCCGCCGGGAGCTCAACCCCATTGTGCTGCTGCT
  TGACAACAAGCCTGACCACCACTTCCTGGAGGCCATCTGCTGCTTCCCCATGGTCTACTACATGGAGGGA
  TCCGTGGACAACCTGGACAGCTTGCTGCAGTGTGGCATCATCTATGCTGACAATCTGGTGGTGGTGGACA
  AGGAGAGTACCATGAGCGCTGAAGAGGACTACATGGCAGATGCCAAGACCATTGTCAACGTGCAGACCAT
  GTTCAGGCTTTTCCCCAGTCTCAGCATCACCACGGAGCTCACGCACCCTTCCAACATGCGGTTCATGCAG
  TTCCGGGCCAAGGACAGCTACTCTCTGGCTCTTTCCAAACTTGAAAAGCAAGAACGGGAGAATGGCTCCA
  ACCTGGCCTTCATGTTCCGCCTGCCATTTGCTGCTGGTCGGGTGTTTAGTATCAGCATGTTGGATACGCT
  GCTCTACCAGTCCTTCGTGAAGGACTACATGATCACCATCACCAGGCTGCTGCTGGGCCTTGACACGACG
  CCAGGCTCTGGCTACCTCTGTGCTATGAAGGTAACTGAGGATGACCTGTGGATCCGTACTTATGGCCGCC
  TCTTCCAGAAACTCTGCTCCTCCAGCGCTGAGATCCCCATCGGCATCTACAGGACCGAGTGCCATGTCTT
  CTCCTCTGAGCCTCATGACCTCAGAGCCCAGTCTCAGATCTCGGTGAACATGGAGGATTGCGAGGACACT
  CGGGAGGCCAAGGGGCCCTGGGGCACACGGGCTGCGTCTGGCGGTGGCAGCACCCATGGCCGTCACGGGG
  GCAGTGCTGACCCGGTGGAGCACCCACTACTGCGCCGCAAGAGCCTGCAGTGGGCCCGCAAGCTGAGCCG
  CAAGAGCAGCAAGCAGGCGGGGAAGGCACCCATGACCACAGACTGGATCACCCAGCAGCGACTCAGCCTG
  TACCGGCGCTCAGAGCGCCAGGAGCTCTCGGAGCTGGTCAAGAACCGAATGAAGCACCTGGGGCTGCCCA
  CCACTGGCTACGAGGACGTAGCAAATTTAACAGCCAGTGATGTGATGAATCGGGTAAACCTGGGATATTT
  GCAAGATGAGATGAATGATCATCACCAGAACACCCTTTCCTATGTACTCATCAACCCCCCGCCAGACACA
  AGACTGGAGCCCAACGATATTGTGTACCTCATCCGTTCCGACCCCCTGGCCCACGTGACCAGCAGCTCCC
  AGAGTCGGAAAAGCAGCTGCAGCAACAAACTGTCATCCTGTAATCCTGAGACCCGGGATGAGACCCAGCT
  CC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201402 representing NM_021853
  Red=Cloning site Green=Tags(s)
  
  MARAKLPRSPSEGKAGPGDTPAGSAAPEEPHGLSPLLPTRGGGSVGSDVGQRLHVEDFSLDSSLSQVQVE
  FYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSS
  SEFHWAPILWVERKMALWVIQVIVATISFLETMLLIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWP
  PLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNL
  NLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRAR
  TEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKD
  QDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCE
  EECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRMYGRCSGNEVYHIRMGDSKFFREYEGKS
  FTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQNRRGLA
  GQALYEGPSRLPVHSIIASMVAMDLQNTDCRPSQGGSGGGGGKLTLPTENGSGSRRPSIAPVLELADSSA
  LLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYE
  DAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEG
  SVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQ
  FRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTT
  PGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSSEPHDLRAQSQISVNMEDCEDT
  REAKGPWGTRAASGGGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSSKQAGKAPMTTDWITQQRLSL
  YRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDT
  RLEPNDIVYLIRSDPLAHVTSSSQSRKSSCSNKLSSCNPETRDETQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021853
• ORF Size: 3711 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021853.1, NP_068625.1
• RefSeq Size: 3714 bp
• RefSeq ORF: 3714 bp
• Locus ID: 60444
• UniProt ID: Q9Z258
• Cytogenetics: 3p13
• MW: 139.6 kDa
• Gene Summary: Na(+)-activated potassium channel; may be involved in regulating the firing properties of neurons [RGD, Feb 2006]